Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,579,384	G→T	R240L (CGA→CTA)	talA →	transaldolase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,579,384	0	G	T	100.0%	293.4 / NA	83	R240L (CGA→CTA)	talA	transaldolase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (54/29);  total (54/29)

TGGAAGAAGATCCGGGCGTTAAATCGGTGCGCAATATCTACGACTACTATAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCA  >  NC_000913/2579252‑2579516                                                                                                                                     |                                                                                                                                     tggAAGAAGATCCGGGCGTTAAATCGGTGCGCAATATCTACGACTACTATAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCtactgact                                                                                                                               >  2:329569/1‑140 (MQ=255)   gaagaaGATCCGGGCGTTAAATCGGTGCGCAATATCTACGACTACTATAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTAt                                                                                                                             >  2:946966/1‑140 (MQ=255)       aagaTCCGGGCGTTAAATCGGTGCGCAATATCTACGACTACTATAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCa                                                                                                                         >  2:703864/1‑140 (MQ=255)           tCCGGGCGTTAAATCGGTGCGCAATATCTACGACTACTATAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGa                                                                                                                     >  1:915275/1‑140 (MQ=255)            ccGGGCGTTAAATCGGTGCGCAATATCTACGACTACTATAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGaa                                                                                                                    <  1:623019/140‑1 (MQ=255)               ggCGTTAAATCGGTGCGCAATATCTACGACTACTATAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAAttt                                                                                                                 >  1:945329/1‑140 (MQ=255)                gCGTTAAATCGGTGCGCAATATCTACGACTACTATAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTa                                                                                                                >  2:665074/1‑140 (MQ=255)                 cGTTAAATCGGTGCGCAATATCTACGACTACTATAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGaa                                                                                                                    >  1:1163217/1‑135 (MQ=255)                 cGTTAAATCGGTGCGCAATATCTACGACTACTATAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGaa                                                                                                                    <  2:1163217/135‑1 (MQ=255)                    tAAATCGGTGCGCAATATCTACGACTACTATAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGa                                                                                                            >  1:465859/1‑140 (MQ=255)                            tGCGCAATATCTACGACTACTATAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTg                                                                                                    <  1:443614/140‑1 (MQ=255)                             gcgcAATATCTACGACTACTATAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGc                                                                                                   >  2:827424/1‑140 (MQ=255)                              cgcAATATCTACGACTACTATAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCa                                                                                                  >  2:911178/1‑140 (MQ=255)                              cgcAATATCTACGACTACTATAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCa                                                                                                  >  1:676653/1‑140 (MQ=255)                                      cTACGACTACTATAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAg                                                                                          >  2:1009785/1‑140 (MQ=255)                                        aCGACTACTATAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGtt                                                                                        >  1:572555/1‑140 (MQ=255)                                           actactATAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCg                                                                                     >  2:1100821/1‑140 (MQ=255)                                            ctactaTAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGc                                                                                    <  2:1157072/140‑1 (MQ=255)                                             tactaTAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGcc                                                                                   >  2:753996/1‑140 (MQ=255)                                             tactaTAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGcc                                                                                   >  1:136283/1‑140 (MQ=255)                                             tactaTAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGcc                                                                                   >  1:557628/1‑140 (MQ=255)                                             tactaTAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGcc                                                                                   >  1:631596/1‑140 (MQ=255)                                               ctaTAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAg                                                                                 >  1:748006/1‑140 (MQ=255)                                               ctaTAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAg                                                                                 >  1:976527/1‑140 (MQ=255)                                                    aGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTa                                                                            >  1:228183/1‑140 (MQ=255)                                                     gCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTAc                                                                           <  2:676653/140‑1 (MQ=255)                                                     gCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTAc                                                                           <  2:572555/140‑1 (MQ=255)                                                      cAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGTACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACg                                                                          >  1:1221939/1‑140 (MQ=255)                                                      cAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACg                                                                          <  2:36297/140‑1 (MQ=255)                                                      cAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACg                                                                          >  2:901395/1‑140 (MQ=255)                                                      cAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACg                                                                          <  1:946966/140‑1 (MQ=255)                                                        acacCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTa                                                                        >  2:833034/1‑140 (MQ=255)                                                         caccacTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTaa                                                                       >  2:608390/1‑140 (MQ=255)                                                         caccacTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTaa                                                                       >  1:16850/1‑140 (MQ=255)                                                         caccacTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTaa                                                                       >  1:1161993/1‑140 (MQ=255)                                                         caccacTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTaa                                                                       >  2:926031/1‑140 (MQ=255)                                                         caccacTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTaa                                                                       >  2:174102/1‑140 (MQ=255)                                                         caccacTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTaa                                                                       >  2:18657/1‑140 (MQ=255)                                                         caccacTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTaa                                                                       >  2:257612/1‑140 (MQ=255)                                                          accacTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGttt                                                                                       <  2:472128/123‑1 (MQ=255)                                                          accacTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGttt                                                                                       >  1:342179/1‑123 (MQ=255)                                                          accacTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGttt                                                                                       >  1:472128/1‑123 (MQ=255)                                                          accacTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGttt                                                                                       <  2:342179/123‑1 (MQ=255)                                                            cacTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAAtt                                                                    >  2:355812/1‑140 (MQ=255)                                                             acTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTa                                                                   <  2:915275/140‑1 (MQ=255)                                                               tATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAAt                                                                 <  2:465859/140‑1 (MQ=255)                                                                aTGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATc                                                                >  2:151947/1‑140 (MQ=255)                                                                 tGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATcc                                                               >  1:767806/1‑140 (MQ=255)                                                                  gAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATccc                                                              <  1:430989/140‑1 (MQ=255)                                                                  gAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATccc                                                              <  1:833034/140‑1 (MQ=255)                                                                    aaCCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAGGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATcc                                                               >  2:1091839/1‑137 (MQ=255)                                                                    aaCCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAGGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATcc                                                               <  1:1091839/137‑1 (MQ=255)                                                                    aaCCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCAc                                                            <  1:329569/140‑1 (MQ=255)                                                                    aaCCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCAc                                                            <  2:325486/140‑1 (MQ=255)                                                                     aCCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCAcc                                                           <  1:753996/140‑1 (MQ=255)                                                                      ccATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCt                                                          >  1:361684/1‑140 (MQ=255)                                                                      ccATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCt                                                          >  1:69606/1‑140 (MQ=255)                                                                         ttGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTtct                                                       >  1:956083/1‑140 (MQ=255)                                                                         ttGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTtct                                                       >  1:365296/1‑140 (MQ=255)                                                                         ttGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTtct                                                       >  1:461320/1‑140 (MQ=255)                                                                         ttGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTtct                                                       >  2:198639/1‑140 (MQ=255)                                                                          tgtgATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTtctc                                                      >  2:838208/1‑140 (MQ=255)                                                                           gtgATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCa                                                     >  2:26334/1‑140 (MQ=255)                                                                                   cgcgAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTcc                                             >  1:36531/1‑140 (MQ=255)                                                                                       aGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACg                                         <  2:945329/140‑1 (MQ=255)                                                                                         cTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGcc                                       <  1:835728/140‑1 (MQ=255)                                                                                            ccGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAg                                    <  1:703864/140‑1 (MQ=255)                                                                                             cGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGc                                   >  1:273774/1‑140 (MQ=255)                                                                                                 gCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTccc                               <  2:273774/140‑1 (MQ=255)                                                                                                   aCCGAACAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCAt                             <  1:355812/140‑1 (MQ=255)                                                                                                        aCAAATCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCg                        <  1:838208/140‑1 (MQ=255)                                                                                                             tCCTCGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCg                   <  2:361684/140‑1 (MQ=255)                                                                                                                 cGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGt               >  1:11282/1‑140 (MQ=255)                                                                                                                 cGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGt               >  2:554325/1‑140 (MQ=255)                                                                                                                 cGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGt               >  1:842740/1‑140 (MQ=255)                                                                                                                 cGCCTTAACCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGt               >  1:604820/1‑140 (MQ=255)                                                                                                                       aaCCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGtt         >  2:290742/1‑140 (MQ=255)                                                                                                                        aCCGGCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTg        >  1:845012/1‑140 (MQ=255)                                                                                                                           ggCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGa     <  2:1161993/140‑1 (MQ=255)                                                                                                                            gCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAg    <  2:767806/140‑1 (MQ=255)                                                                                                                            gCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAg    >  2:384531/1‑140 (MQ=255)                                                                                                                            gCTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAg    <  2:956083/140‑1 (MQ=255)                                                                                                                             cTGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGc   >  2:833099/1‑140 (MQ=255)                                                                                                                              tGCGATCTACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGca  <  1:911178/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                     TGGAAGAAGATCCGGGCGTTAAATCGGTGCGCAATATCTACGACTACTATAAGCAACACCACTATGAAACCATTGTGATGGGCGCGAGCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCA  >  NC_000913/2579252‑2579516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑