Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,494,239	C→A	L77M (CTG→ATG)	nirB →	nitrite reductase catalytic subunit NirB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,494,239	0	C	A	100.0%	282.7 / NA	80	L77M (CTG→ATG)	nirB	nitrite reductase catalytic subunit NirB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (38/42);  total (38/42)

ACCGTTTTCTGTGAAGAACCGCGCATCGCTTATGACCGCGTACACCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTCTGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTCCTACCCGTGGATCCCGCCAATCAAAGGTTCTG  >  NC_000913/3494110‑3494374                                                                                                                                  |                                                                                                                                        aCCGTTTTCTGTGAAGAACCGCGCATCGCTTATGACCGCGTACACCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGa                                                                                                                               >  2:1202525/1‑140 (MQ=255) aCCGTTTTCTGTGAAGAACCGCGCATCGCTTATGACCGCGTACACCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGa                                                                                                                               >  2:455455/1‑140 (MQ=255) aCCGTTTTCTGTGAAGAACCGCGCATCGCTTATGACCGCGTACACCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGa                                                                                                                               >  2:862367/1‑140 (MQ=255)  ccGTTTTCTGTGAAGAACCGCGCATCGCTTATGACCGCGTACACCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGaa                                                                                                                              <  2:328646/140‑1 (MQ=255)  ccGTTTTCTGTGAAGAACCGCGCATCGCTTATGACCGCGTACACCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGaa                                                                                                                              <  1:428246/140‑1 (MQ=255)  ccGTTTTCTGTGAAGAACCGCGCATCGCTTATGACCGCGTACACCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGaa                                                                                                                              >  1:456182/1‑140 (MQ=255)  ccGTTTTCTGTGAAGAACCGCGCATCGCTTATGACCGCGTACACCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCCGCGaa                                                                                                                              <  2:963034/140‑1 (MQ=255)     ttttCTGTGAAGAACCGCGCATCGCTTATGACCGCGTACACCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAAcgc                                                                                                                           >  2:26155/1‑140 (MQ=255)      tttCTGTGAAGAACCGCGCATCGCTTATGACCGCGTACACCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAAcgcg                                                                                                                          <  1:288505/140‑1 (MQ=255)      tttCTGTGAAGAACCGCGCATCGCTTATGACCGCGTACACCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAAcgcg                                                                                                                          >  2:625327/1‑140 (MQ=255)      tttCTGTGAAGAACCGCGCATCGCTTATGACCGCGTACACCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAAcgcg                                                                                                                          <  1:1152095/140‑1 (MQ=255)        tCTGTGAAGAACCGCGCATCGCTTATGACCGCGTACACCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCt                                                                                                                        >  1:463993/1‑140 (MQ=255)          tgtgAAGAACCGCGCATCGCTTATGACCGCGTACACCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCt                                                                                                                        >  1:297563/1‑138 (MQ=255)          tgtgAAGAACCGCGCATCGCTTATGACCGCGTACACCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCt                                                                                                                        <  2:297563/138‑1 (MQ=255)                  aCCGCGCATCGCTTATGACCGCGTACACCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCa                                                                                                              <  1:1236786/140‑1 (MQ=255)                  aCCGCGCATCGCTTATGACCGCGTACACCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCa                                                                                                              <  1:1093939/140‑1 (MQ=255)                      cgcATCGCTTATGACCGCGTACACCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCg                                                                                                          >  1:408106/1‑140 (MQ=255)                      cgcATCGCTTATGACCGCGTACACCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCg                                                                                                          >  1:547688/1‑140 (MQ=255)                        cATCGCTTATGACCGCGTACACCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTc                                                                                                        >  2:655201/1‑140 (MQ=255)                            gCTTATGACCGCGTACACCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGga                                                                                                    <  2:22579/140‑1 (MQ=255)                                   aCCGCGTACACCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTg                                                                                             <  1:26155/140‑1 (MQ=255)                                     cgcgTACACCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGAt                                                                                           >  2:1136633/1‑140 (MQ=255)                                      gcgTACACCTCTCGTCTTACTTCTCTAACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGAtt                                                                                          <  1:331706/140‑1 (MQ=255)                                          acacCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACt                                                                                      <  1:119344/140‑1 (MQ=255)                                           cacCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTc                                                                                     <  2:1038095/140‑1 (MQ=255)                                           cacCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTc                                                                                     <  2:860536/140‑1 (MQ=255)                                              ctctCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAg                                                                                  >  1:887366/1‑140 (MQ=255)                                                ctcGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAgcg                                                                                <  2:131633/140‑1 (MQ=255)                                                ctcGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAgcg                                                                                >  1:639540/1‑140 (MQ=255)                                                 tcGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAgcgc                                                                               >  1:866652/1‑140 (MQ=255)                                                        aCTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAgcgc                                                                               >  1:211945/1‑133 (MQ=255)                                                        aCTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAgcgc                                                                               <  2:211945/133‑1 (MQ=255)                                                        aCTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGt                                                                        >  1:732185/1‑140 (MQ=255)                                                        aCTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGt                                                                        >  1:818297/1‑140 (MQ=255)                                                        aCTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGt                                                                        >  2:989579/1‑140 (MQ=255)                                                         cTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTa                                                                       >  2:1011161/1‑140 (MQ=255)                                                           tctctcACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAGGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTAcc                                                                     >  2:20180/1‑140 (MQ=255)                                                                caccacACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGtttt                                                                <  2:508468/140‑1 (MQ=255)                                                                 accacACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGttttt                                                               >  1:1239403/1‑140 (MQ=255)                                                                   cacaCCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCg                                                                    <  1:1166144/133‑1 (MQ=255)                                                                   cacaCCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCg                                                                    >  2:1166144/1‑133 (MQ=255)                                                                    acacCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTAt                                                            >  1:475610/1‑140 (MQ=255)                                                                     cacCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATg                                                           <  1:1036955/140‑1 (MQ=255)                                                                     cacCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATg                                                           <  2:475610/140‑1 (MQ=255)                                                                      acCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGa                                                          >  2:446243/1‑140 (MQ=255)                                                                      acCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGa                                                          <  2:315437/140‑1 (MQ=255)                                                                      acCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGa                                                          <  1:862367/140‑1 (MQ=255)                                                                      acCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGa                                                          >  1:299973/1‑140 (MQ=255)                                                                      acCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGa                                                          <  2:299973/140‑1 (MQ=255)                                                                      acCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGa                                                          >  2:79509/1‑140 (MQ=255)                                                                      acCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGa                                                          <  1:104637/140‑1 (MQ=255)                                                                       ccgccgAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGAc                                                         <  2:278151/140‑1 (MQ=255)                                                                          ccgAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAg                                                      <  1:655201/140‑1 (MQ=255)                                                                          ccgAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAg                                                      <  2:490213/140‑1 (MQ=255)                                                                             aaGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACAATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTg                                                   >  2:1129419/1‑140 (MQ=255)                                                                              agagCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGa                                                  <  2:1233453/140‑1 (MQ=255)                                                                                agCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATc                                                >  1:181347/1‑140 (MQ=255)                                                                                  cTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGGTTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCAt                                              >  2:1039531/1‑140 (MQ=255)                                                                                   tGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATg                                             <  1:79509/140‑1 (MQ=255)                                                                                    gTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTTGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATgg                                            >  1:1006146/1‑140 (MQ=255)                                                                                    gTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATgg                                            <  2:408106/140‑1 (MQ=255)                                                                                     tCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATGGc                                           >  1:1155310/1‑140 (MQ=255)                                                                                            tGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGt                                    <  1:455455/140‑1 (MQ=255)                                                                                               gcgAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTcc                                 <  2:815186/140‑1 (MQ=255)                                                                                               gcgAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTcc                                 <  1:412432/140‑1 (MQ=255)                                                                                               gcgAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTcc                                 <  2:866652/140‑1 (MQ=255)                                                                                               gcgAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTcc                                 <  1:1202525/140‑1 (MQ=255)                                                                                                cgAAGGCTTCTACGAGAAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTCCt                                <  2:818297/140‑1 (MQ=255)                                                                                                               gaAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTCCTACCCGTGGAt                      >  1:1019111/1‑135 (MQ=255)                                                                                                               gaAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTCCTACCCGTGGAt                      <  2:1019111/135‑1 (MQ=255)                                                                                                               gaAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTCCTACCCGTGGATCCCGc                 >  2:574250/1‑140 (MQ=255)                                                                                                               gaAACACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTCCTACCCGTGGATCCCGc                 >  1:237640/1‑140 (MQ=255)                                                                                                                 aaCACGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTCCTACCCGTGGATCCCGCCa               <  2:1239403/140‑1 (MQ=255)                                                                                                                  acacGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTCCTACCCGTGGATCCCGCCaa              >  1:666437/1‑140 (MQ=255)                                                                                                                     cGGCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTCCTACCCGTGGATCCCGCCAATCa           <  2:237640/140‑1 (MQ=255)                                                                                                                      ggCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTCCTACCCGTGGATCCCGCCAATCaa          >  2:603634/1‑140 (MQ=255)                                                                                                                      ggCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTCCTACCCGTGGATCCCGCCAATCaa          >  2:998674/1‑140 (MQ=255)                                                                                                                       gCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTCCTGCCCGTGGATCCCGCCAATCaaa         <  2:181347/140‑1 (MQ=255)                                                                                                                       gCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTCCTACCCGTGGATCCCGCCa               >  2:1196020/1‑134 (MQ=255)                                                                                                                       gCATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTCCTACCCGTGGATCCCGCCa               <  1:1196020/134‑1 (MQ=255)                                                                                                                        cATCAAAGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCCCTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATGGCACCCGGTTCCTA‑CCGTGGATCCCGCCAATCAAAgg       <  2:463993/140‑1 (MQ=255)                                                                                                                            aaaGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTCCTACCCGTGGATCCCGCCAATCAAAGGTTc    <  2:639540/140‑1 (MQ=255)                                                                                                                              aGTTATGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGGTTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTCCTACCCGTGGATCCCGCCAATCAAAGGTTCTg  <  1:1039531/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                        ACCGTTTTCTGTGAAGAACCGCGCATCGCTTATGACCGCGTACACCTCTCGTCTTACTTCTCTCACCACACCGCCGAAGAGCTGTCGCTGGTGCGCGAAGGCTTCTACGAGAAACACGGCATCAAAGTTCTGGTCGGCGAACGCGCTATCACCATCAACCGTCAGGAGAAGGTGATTCACTCCAGCGCCGGACGTACCGTTTTTTATGACAAGCTGATCATGGCAACCGGTTCCTACCCGTGGATCCCGCCAATCAAAGGTTCTG  >  NC_000913/3494110‑3494374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑