Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	409,980	G→T	R14S (CGC→AGC)	rdgC ←	nucleoid‑associated protein RdgC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	409,980	0	G	T	100.0%	238.0 / NA	67	R14S (CGC→AGC)	rdgC	nucleoid‑associated protein RdgC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (30/37);  total (30/37)

CGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGTCCGTCTCTACGCTATT  >  NC_000913/409845‑410116                                                                                                                                        |                                                                                                                                         cGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTa                                                                                                                                      >  2:488882/1‑140 (MQ=255) cGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTa                                                                                                                                      <  2:359689/140‑1 (MQ=255) cGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTa                                                                                                                                      <  1:974824/140‑1 (MQ=255) cGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCAGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTa                                                                                                                                      <  1:201572/140‑1 (MQ=255)  gTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTaa                                                                                                                                     <  1:1122651/140‑1 (MQ=255)  gTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTaa                                                                                                                                     <  2:332009/140‑1 (MQ=255)    gCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGa                                                                                                                                   >  2:66886/1‑140 (MQ=255)     cGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGAc                                                                                                                                  >  2:672178/1‑140 (MQ=255)     cGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGAc                                                                                                                                  >  1:818280/1‑140 (MQ=255)         aaCGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTa                                                                                                                              <  1:385334/140‑1 (MQ=255)                    gtgCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAAt                                                                                                                   <  1:1011256/140‑1 (MQ=255)                    gtgCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAAt                                                                                                                   <  2:1054468/140‑1 (MQ=255)                     tgCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAAtt                                                                                                                  >  1:1049367/1‑140 (MQ=255)                     tgCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGGAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAAtt                                                                                                                  >  1:836309/1‑140 (MQ=255)                      gCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAAttt                                                                                                                 <  2:811818/140‑1 (MQ=255)                          tCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGa                                                                                                             >  1:283627/1‑140 (MQ=255)                          tCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGa                                                                                                             >  1:407459/1‑140 (MQ=255)                          tCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGa                                                                                                             >  2:430225/1‑140 (MQ=255)                           cccATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGaa                                                                                                            >  2:587268/1‑140 (MQ=255)                             cATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAAcc                                                                                                          >  1:1072250/1‑140 (MQ=255)                              aTCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACca                                                                                                         <  2:994585/140‑1 (MQ=255)                              aTCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACca                                                                                                         <  2:780237/140‑1 (MQ=255)                              aTCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACca                                                                                                         <  2:283627/140‑1 (MQ=255)                                 ggaggaACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAg                                                                                                      <  2:46527/140‑1 (MQ=255)                                       aCCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATAGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGAt                                                                                                >  1:334900/1‑140 (MQ=255)                                         ccAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCAt                                                                                                   <  2:458216/135‑1 (MQ=255)                                         ccAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCAt                                                                                                   >  1:458216/1‑135 (MQ=255)                                            gCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAAttt                                                                                           <  1:1186814/140‑1 (MQ=255)                                             cccATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAACGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTc                                                                                          >  2:31675/1‑140 (MQ=255)                                               cATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAACCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCa                                                                                        <  2:551938/140‑1 (MQ=255)                                                           gTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGt                                                                            >  1:647968/1‑140 (MQ=255)                                                           gTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGt                                                                            <  1:488882/140‑1 (MQ=255)                                                             ccTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTa                                                                          >  2:702807/1‑140 (MQ=255)                                                                      cGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCg                                                                 >  1:27952/1‑140 (MQ=255)                                                                      cGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCg                                                                 >  1:916194/1‑140 (MQ=255)                                                                        cATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCggg                                                               <  1:702807/140‑1 (MQ=255)                                                                         aTGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGc                                                              <  1:587268/140‑1 (MQ=255)                                                                         aTGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGc                                                              <  2:844520/140‑1 (MQ=255)                                                                         aTGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGc                                                              <  2:1072250/140‑1 (MQ=255)                                                                          tGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCa                                                             <  2:63578/140‑1 (MQ=255)                                                                            gggTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATg                                                           <  2:684137/140‑1 (MQ=255)                                                                              gTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGAt                                                         <  2:27952/140‑1 (MQ=255)                                                                                       aTCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTg                                                >  2:1228476/1‑140 (MQ=255)                                                                                       aTCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTg                                                >  2:66541/1‑140 (MQ=255)                                                                                         cGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTc                                              >  1:519892/1‑140 (MQ=255)                                                                                           aGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTcgg                                            <  2:703768/140‑1 (MQ=255)                                                                                             gCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTcggcg                                          >  1:273877/1‑140 (MQ=255)                                                                                               tAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCggcggc                                        <  2:836309/140‑1 (MQ=255)                                                                                               tAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCggcggc                                        <  2:1049367/140‑1 (MQ=255)                                                                                                  cTGTTGTTCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCtt                                     >  1:741307/1‑140 (MQ=255)                                                                                                      ttttCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCAt                                 <  1:430225/140‑1 (MQ=255)                                                                                                       tttCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCAtt                                <  1:1153257/140‑1 (MQ=255)                                                                                                         tCCATCTCTTCTGCACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGc                              <  1:963208/140‑1 (MQ=255)                                                                                                                      cACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGTc                 <  2:827925/140‑1 (MQ=255)                                                                                                                      cACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGTc                 <  1:66541/140‑1 (MQ=255)                                                                                                                      cACGCAGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGTc                 <  2:916194/140‑1 (MQ=255)                                                                                                                           aacGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGTCCGtct            <  1:31675/138‑1 (MQ=255)                                                                                                                           aGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGTc                 >  1:42808/1‑135 (MQ=255)                                                                                                                           aGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGTc                 <  2:42808/135‑1 (MQ=255)                                                                                                                           aGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGTCCGtct            >  1:743189/1‑140 (MQ=255)                                                                                                                           aGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGTCCGtct            >  1:213171/1‑140 (MQ=255)                                                                                                                           aGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGTCCGtct            >  1:1242407/1‑140 (MQ=255)                                                                                                                           aGCGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGTCCGtct            >  1:1172637/1‑140 (MQ=255)                                                                                                                             cGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGTCCGTctct          >  1:1084304/1‑140 (MQ=255)                                                                                                                             cGAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGTCCGTctct          >  2:424308/1‑140 (MQ=255)                                                                                                                              gAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGTCCGTCTCTa         <  2:1029020/140‑1 (MQ=255)                                                                                                                              gAAATCTCGCTGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGTCCGTCTCTa         <  2:1172637/140‑1 (MQ=255)                                                                                                                                     cgctGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGTCCGTCTCTACGCtatt  >  2:797122/1‑140 (MQ=255)                                                                                                                                     cgctGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGTCCGTCTCTACGCtatt  <  1:1228476/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         CGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGTCCGTCTCTACGCTATT  >  NC_000913/409845‑410116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑