Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,342,124	C→A	I68I (ATC→ATA)	pyrF →	orotidine‑5'‑phosphate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,342,124	0	C	A	100.0%	261.1 / NA	74	I68I (ATC→ATA)	pyrF	orotidine‑5'‑phosphate decarboxylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (40/34);  total (40/34)

TCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCA  >  NC_000913/1341989‑1342259                                                                                                                                        |                                                                                                                                        tCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATAtatttc                                                                                                                                     <  2:231417/140‑1 (MQ=255)   aTAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATAtatttctt                                                                                                                                   >  1:399754/1‑140 (MQ=255)      aTCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGAc                                                                                                                                >  1:1032288/1‑140 (MQ=255)          tgatgaCGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGa                                                                                                                            <  1:980296/140‑1 (MQ=255)           gatgaCGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGaa                                                                                                                           <  2:598066/140‑1 (MQ=255)           gatgaCGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGaa                                                                                                                           >  1:549011/1‑140 (MQ=255)            atgaCGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGaaa                                                                                                                          >  2:592798/1‑140 (MQ=255)            atgaCGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGaaa                                                                                                                          >  2:310993/1‑140 (MQ=255)            atgaCGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGaaa                                                                                                                          >  1:237717/1‑140 (MQ=255)             tgaCGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAAt                                                                                                                         >  1:81456/1‑140 (MQ=255)               aCGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATAtatttct                                                                                                                                    <  2:785535/127‑1 (MQ=255)               aCGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATAtatttct                                                                                                                                    >  1:785535/1‑127 (MQ=255)                cgcgCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTcc                                                                                                                      >  1:629885/1‑140 (MQ=255)                   gcTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACg                                                                                                                   <  2:81456/140‑1 (MQ=255)                   gcTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACg                                                                                                                   <  2:907159/140‑1 (MQ=255)                   gcTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACg                                                                                                                   <  1:777032/140‑1 (MQ=255)                   gcTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACg                                                                                                                   <  1:494201/140‑1 (MQ=255)                   gcTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACg                                                                                                                   <  1:229570/140‑1 (MQ=255)                     tGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGat                                                                                                                 <  2:1117877/140‑1 (MQ=255)                     tGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGat                                                                                                                 <  2:1003942/140‑1 (MQ=255)                     tGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGat                                                                                                                 <  1:893021/140‑1 (MQ=255)                     tGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGat                                                                                                                 <  1:104330/140‑1 (MQ=255)                           ttGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATcccc                                                                                                           >  1:112063/1‑140 (MQ=255)                             gTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCaa                                                                                                         >  2:962132/1‑140 (MQ=255)                             gTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCaa                                                                                                         >  2:235063/1‑140 (MQ=255)                                 aCAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACt                                                                                                     >  1:204276/1‑140 (MQ=255)                                    aGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCa                                                                                                  <  2:237717/140‑1 (MQ=255)                                    aGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCa                                                                                                  <  2:400797/140‑1 (MQ=255)                                      aTCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAgc                                                                                                >  1:287840/1‑140 (MQ=255)                                        cGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAgcgc                                                                                              >  1:358968/1‑140 (MQ=255)                                           cccACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACg                                                                                           >  1:608019/1‑140 (MQ=255)                                               cgcgATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGt                                                                                       >  1:1180272/1‑140 (MQ=255)                                                gcgATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGAAATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTc                                                                                      <  2:1042071/140‑1 (MQ=255)                                                 cgATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCg                                                                                     <  1:841335/140‑1 (MQ=255)                                                     tGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCgctgc                                                                                 >  2:504444/1‑140 (MQ=255)                                                         gtcTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCa                                                                             <  1:134955/140‑1 (MQ=255)                                                         gtcTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCa                                                                             <  1:250653/140‑1 (MQ=255)                                                          tcTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAg                                                                            <  1:216760/140‑1 (MQ=255)                                                          tcTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAg                                                                            <  2:1032288/140‑1 (MQ=255)                                                           cTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGc                                                                           <  1:760041/140‑1 (MQ=255)                                                              aaGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGa                                                                        >  2:1264493/1‑140 (MQ=255)                                                                ggTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACt                                                                      <  2:914272/140‑1 (MQ=255)                                                                 gTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACtt                                                                     >  1:721693/1‑140 (MQ=255)                                                                    ggCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAgg                                                                  <  2:358968/140‑1 (MQ=255)                                                                    ggCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAgg                                                                  >  2:823203/1‑140 (MQ=255)                                                                    ggCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATAT‑CCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGc                                                                 <  2:534418/140‑1 (MQ=255)                                                                      cAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCg                                                                >  2:1262428/1‑140 (MQ=255)                                                                          gagaTGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGtgtg                                                            <  1:962132/140‑1 (MQ=255)                                                                             aTGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGAt                                                         <  1:840487/140‑1 (MQ=255)                                                                                     aTTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATg                                                 >  1:399376/1‑140 (MQ=255)                                                                                     aTTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATg                                                 >  1:162551/1‑140 (MQ=255)                                                                                        gTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTc                                              <  2:16136/140‑1 (MQ=255)                                                                                         tttGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCa                                             >  1:949644/1‑140 (MQ=255)                                                                                         tttGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCa                                             >  1:1213437/1‑140 (MQ=255)                                                                                          ttGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCAt                                            <  1:310993/140‑1 (MQ=255)                                                                                          ttGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCAt                                            >  1:25947/1‑140 (MQ=255)                                                                                          ttGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCAt                                            >  1:211857/1‑140 (MQ=255)                                                                                                   aGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGa                                                    >  1:1270051/1‑123 (MQ=255)                                                                                                   aGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGa                                                    <  2:1270051/123‑1 (MQ=255)                                                                                                   aGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGtt                                               >  2:146386/1‑128 (MQ=255)                                                                                                   aGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGtt                                               <  1:146386/128‑1 (MQ=255)                                                                                                   aGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCtggt                                   <  1:250187/140‑1 (MQ=255)                                                                                                   aGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCtggt                                   >  1:1002821/1‑140 (MQ=255)                                                                                                   aGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCtggt                                   >  2:681083/1‑140 (MQ=255)                                                                                                       tgtgCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTgggg                               >  1:1133183/1‑140 (MQ=255)                                                                                                        gtgCGCGAACTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGgc                              >  1:1196469/1‑140 (MQ=255)                                                                                                               aaCTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTatg                       >  1:1060252/1‑140 (MQ=255)                                                                                                               aaCTTCAACAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTatg                       >  2:762409/1‑140 (MQ=255)                                                                                                                       cAGCGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGc               <  2:204276/140‑1 (MQ=255)                                                                                                                          cGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCa              <  1:865766/138‑1 (MQ=255)                                                                                                                          cGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCa              >  2:865766/1‑138 (MQ=255)                                                                                                                          cGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAgc            >  1:1274397/1‑140 (MQ=255)                                                                                                                          cGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAgc            >  2:882631/1‑140 (MQ=255)                                                                                                                          cGTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAgc            >  1:788912/1‑140 (MQ=255)                                                                                                                           gTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAgcg           >  2:1134173/1‑140 (MQ=255)                                                                                                                           gTGGTTTTGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAgcg           >  1:914189/1‑140 (MQ=255)                                                                                                                               ttttGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTg       <  1:504444/140‑1 (MQ=255)                                                                                                                                tttGATATATTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGa      <  1:681083/140‑1 (MQ=255)                                                                                                                                    atatatTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCa  <  1:1264493/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCA  >  NC_000913/1341989‑1342259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑