| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 991,298 | 0 | C | A | 21.1% | 106.6 / 18.2 | 38 | A226E (GCG→GAG) | pepN | aminopeptidase N |
| Reads supporting (aligned to +/- strand): ref base C (16/14); new base A (3/5); total (19/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACATCTATATGATCGTCGCGGTGGATTTCTTCAATATGGGCGCAATGGAGAATAAGGGGCTGAATATCTTTA > NC_000913/991159‑991435 | ttCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGc > 1:110481/1‑140 (MQ=255) tGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCt > 2:116253/1‑140 (MQ=255) gTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACt > 1:52480/1‑140 (MQ=255) cGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGAGATGACCTCGCTGAAAAACTCCATGAAATGGGATgaag > 2:147537/1‑140 (MQ=255) aCTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATgaagaa > 1:185961/1‑140 (MQ=255) gTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGAGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTgg < 1:329925/140‑1 (MQ=255) tACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGc < 1:276019/140‑1 (MQ=255) aCTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGcc < 1:116253/140‑1 (MQ=255) cTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCt > 2:223325/1‑140 (MQ=255) tGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTg > 1:63015/1‑140 (MQ=255) cgcgATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGa > 2:49606/1‑140 (MQ=255) cgcgATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGa > 2:269154/1‑140 (MQ=255) cgcgATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGa > 1:202205/1‑140 (MQ=255) cgcgATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGAGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGa > 1:332150/1‑140 (MQ=255) cgATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGAGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGt > 1:316424/1‑140 (MQ=255) aCCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGa > 1:189655/1‑140 (MQ=255) ccACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGAc < 1:223325/140‑1 (MQ=255) ccACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGAGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGAc < 1:147537/140‑1 (MQ=255) cgTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTc > 2:99330/1‑132 (MQ=255) cgTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTc < 1:99330/132‑1 (MQ=255) cgTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCg < 1:44740/133‑1 (MQ=255) cgTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCg > 2:44740/1‑133 (MQ=255) cgTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACATCta > 1:208424/1‑140 (MQ=255) cgTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACATCta > 2:327487/1‑140 (MQ=255) tCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACATCTatat > 2:208661/1‑140 (MQ=255) aGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACATCTATATGATCGTCGCGGTgg < 1:208661/140‑1 (MQ=255) gCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACATCTATATGATCGTCGCGGTGGa < 2:110481/140‑1 (MQ=255) gCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACATCTATATGATCGTCGCGGTGGa < 2:189655/140‑1 (MQ=255) cACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACATCTATATGATCGTCGCGGTGGAt < 2:52480/140‑1 (MQ=255) cACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGAGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACATCTATATGATCGTCGCGGTGGAt < 1:265212/140‑1 (MQ=255) cACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGAGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACATCTATATGATCGTCGCGGTGGAt < 1:265213/140‑1 (MQ=255) gAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACATCTATATGATCGTCGCGGTGGATttctt > 2:276995/1‑140 (MQ=255) aGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACATCTATATGATCGTCGCGGTGGATttcttc < 1:269154/140‑1 (MQ=255) cgcgGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACATCTATATGATCGTCGCGGTGGATTTCTTCAATATGGGCGCAAt < 2:275319/140‑1 (MQ=255) gcgGCAGCCTTGATCGCGCGCCGTGGGCGATGCCCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACATCTATATGATCGTCGCGGTGGATTTCTTCAATATGGGCGCAATg < 2:227227/140‑1 (MQ=255) gATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACATCTATATGATCGTCGCGGTGGATTTCTTCAATATGGGCGCAATGGAGAATAAggg < 2:208424/140‑1 (MQ=255) aTCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACATCTATATGATCGTCGCGGTGGATTTCTTCAATATGGGCGCAATGGAGAATAAgggg < 1:327487/140‑1 (MQ=255) cgcCGTGGGAGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACATCTATATGATCGTCGCGGTGGATTTCTTCAATATGGGCGCAATGGAGAATAAGGGGCTGAat < 2:316424/140‑1 (MQ=255) ggCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACATCTATATGATCGTCGCGGTGGATTTCTTCAATATGGGCGCAATGGAGAATAAGGGGCTGAATATCTTTa > 1:305243/1‑140 (MQ=255) | TTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACATCTATATGATCGTCGCGGTGGATTTCTTCAATATGGGCGCAATGGAGAATAAGGGGCTGAATATCTTTA > NC_000913/991159‑991435 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |