Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,362,906	G→T	A243D (GCC→GAC)	yfaY ←	IPR008135 CinA family protein YfaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,362,906	0	G	T	100.0%	33.0 / NA	11	A243D (GCC→GAC)	yfaY	IPR008135 CinA family protein YfaY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (3/8);  total (3/8)

GCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAACTGGCGGTTTTGCAATTCGCGACTGATCTGCGCGGGCAGTCCTTCAGTGCCTTCGAAAATCACGCTCTGTCCGGCAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGATAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGA  >  NC_000913/2362771‑2363034                                                                                                                                        |                                                                                                                                 gcgcGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAACTGGCGGTTTTGCAATTCGCGACTGATCTGCGCGGGCAGTCCTTCAGTGCCTTCGAAAATCACGCTCTGTCCGTCAac                                                                                                                              >  1:250234/1‑140 (MQ=255)                 aaGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAACTGGCGGTTTTGCAATTCGCGACTGATCTGCGCGGGCAGTCCTTCAGTGCCTTCGAAAATCACGCTCTGTCCGTCAACACGTTTAACATCCAGc                                                                                                              <  2:250234/140‑1 (MQ=255)                              accGGTGAATTGCTCGCTCAACGTCAGGCTGAACTGGCGGTTTTGCAATTCGCGACTGATCTGCGCGGGCAGTCCTTCAGTGCCTTCGAAAATCACGCTCTGTCCGTCAACACGTTTAACATCCAGCCACAGTTTTTCCa                                                                                                 <  1:23693/140‑1 (MQ=255)                                                 aaCGTCAGGCTGAACTGGCGGTTTTGCAATTCGCGACTGATCTGCGCGGGCAGTCCTTCAGTGCCTTCGAAAATCACGCTCTGTCCGTCAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGc                                                                              <  2:232662/140‑1 (MQ=255)                                                         gCTGAACTGGCGGTTTTGCAATTCGCGGCTGATCTGCGCGGGCAGTCCTTCAGTGCCTTCGAAAATCACGCTCTGTCCGTCAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGccggtccgg                                                                      >  2:216001/1‑140 (MQ=255)                                                                     gTTTTGCAATTCGCGACTGATCTGCGCGGGCAGTCCTTCAGTGCCTTCGAAAATCACGCTCTGTCCGTCAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGtt                                                          <  1:17788/140‑1 (MQ=255)                                                                                        aTCTGCGCGGGCAGTCCTTCAGTGCCTTCGAAAATCACGCTCTGTCCGTCAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGATAGGCATTgagga                                       >  1:135247/1‑140 (MQ=255)                                                                                        aTCTGCGCGGGCAGTCCTTCAGTGCCTTCGAAAATCACGCTCTGTCCGTCAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGATAGGCATTgagga                                       >  1:6588/1‑140 (MQ=255)                                                                                                  gCAGTCCTTCAGTGCCTTCGAAAATCACGCTCTGTCCGTCAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGATAGGCATTGAGGAGCGATAGccc                             >  2:20612/1‑140 (MQ=255)                                                                                                  gCAGTCCTTCAGTGCCTTCGAAAATCACGCTCTGTCCGTCAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGATAGGCATTGAGGAGCGATAGccc                             <  2:308506/140‑1 (MQ=255)                                                                                                            aGTGCCTTCGAAAATCACGCTCTGTCCGTCAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGATAGGCATTGAGGAGCGATAGCCCATTGTTACGc                   <  2:135247/140‑1 (MQ=255)                                                                                                                             cGCTCTGTCCGTCAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGATAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTaga  <  1:15096/140‑1 (MQ=255)                                                                                                                             cGCTCTGTCCGTCAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGATAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTaga  <  2:6588/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                 GCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAACTGGCGGTTTTGCAATTCGCGACTGATCTGCGCGGGCAGTCCTTCAGTGCCTTCGAAAATCACGCTCTGTCCGGCAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGATAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGA  >  NC_000913/2362771‑2363034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑