Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,414,528	C→A	G353G (GGC→GGA)	ackA →	acetate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,414,528	0	C	A	100.0%	35.6 / NA	12	G353G (GGC→GGA)	ackA	acetate kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (7/5);  total (7/5)

ATGGATGGTCGTCTGGACGCTGTTGTATTCACTGGTGGTATCGGTGAAAATGCCGCAATGGTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGCTTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTA  >  NC_000913/2414430‑2414640                                                                                                   |                                                                                                                 atggatggTCGTCTGGACGCTGTTGTATTCACTGGTGGTATCGGTGAAAATGCCGCAATGGTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGATTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCgg                                                                         >  1:91132/1‑140 (MQ=255)                  cGCTGTTGTATTCACTGGTGGTATCGGTGAAAATGCCGCAATGGTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGATTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTtcatca                                                        >  1:340750/1‑140 (MQ=255)                  cGCTGTTGTATTCACTGGTGGTATCGGTGAAAATGCCGCAATGGTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGATTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTtcatca                                                        >  2:66682/1‑140 (MQ=255)                          tATTCCCTGGTGGTATCGGTGAAAATGCCGCAATGGTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGATTTGAAGTTGATCATGAACGCATCCTGGCTGCACGTTTCGGGAAATCTGGTTTCATCAACAaagaa                                                <  2:301936/140‑1 (MQ=255)                                    tggtATCGGTGAAAATGCCGCAATGGTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGATTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTc                                      >  1:154824/1‑140 (MQ=255)                                    tggtATCGGTGAAAATGCCGCAATGGTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGATTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTc                                      >  2:262704/1‑140 (MQ=255)                                      gtATCGGTGAAAATGCCGCAATGGTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGATTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCt                                    >  1:146615/1‑140 (MQ=255)                                                 aaTGCCGCAATGGTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGATTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGc                                  <  1:338602/131‑1 (MQ=255)                                                 aaTGCCGCAATGGTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGATTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGc                                  >  2:338602/1‑131 (MQ=255)                                                            ggTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGATTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACg              <  2:154824/140‑1 (MQ=255)                                                            ggTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGATTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACg              <  2:291739/140‑1 (MQ=255)                                                              ttCGTGAACTGTCCCTGGGCAAACTGGGCGTGCTGGGATTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACgaa            <  2:86230/140‑1 (MQ=255)                                                                        gTCTCAGGGCAAACTAGGCGTGCTGGGATTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTa  <  2:162296/140‑1 (MQ=255)                                                                                                   |                                                                                                                 ATGGATGGTCGTCTGGACGCTGTTGTATTCACTGGTGGTATCGGTGAAAATGCCGCAATGGTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGCTTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTA  >  NC_000913/2414430‑2414640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑