Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,654,245	C→A	L95L (CTC→CTA)	slp →	starvation lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,654,245	0	C	A	100.0%	96.8 / NA	29	L95L (CTC→CTA)	slp	starvation lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (13/16);  total (13/16)

GTCAACAAGCGCGCTTTGGTGGGAAGGTTATCAACGTTATCAATGGCAAAACGGATACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTCGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGAACAACCTGGCTTTATCAATAAAGTCCCGTATAACTTCCTGGAAGTGAATATGCAGGG  >  NC_000913/3654109‑3654379                                                                                                                                         |                                                                                                                                       gTCAACAAGCGCGCTTTGGTGGGAAGGTTATCAACGTTATCAATGGCAAAACGGATACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGcc                                                                                                                                     >  1:295399/1‑140 (MQ=255)    aacaaGCGCGCTTTGGTGGGAAGGTTATCAACGTTATCAATGGCAAAACGGATACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGa                                                                                                                                  >  2:12632/1‑140 (MQ=255)            cgcTTTGGTGGGAAGGTTATCAACGTTATCAATGGCAAAACGGATACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATA‑TGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGc                                                                                                                         <  1:118901/140‑1 (MQ=255)                             tATCAACGTTATCAATGGCAAAACGGATACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGa                                                                                                         >  2:198933/1‑140 (MQ=255)                                         cAATGGCAAAACGGATACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATc                                                                                             <  1:61766/140‑1 (MQ=255)                                          aaTGGCAAAACGGATACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCa                                                                                            <  1:12632/140‑1 (MQ=255)                                            tGGCAAAACGGATACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACt                                                                                          <  2:2238/140‑1 (MQ=255)                                               cAAAACGGATACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGaa                                                                                                        <  2:335942/123‑1 (MQ=255)                                               cAAAACGGATACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGaa                                                                                                        >  1:335942/1‑123 (MQ=255)                                                       aTACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACtt                                                                                         <  2:145993/130‑1 (MQ=255)                                                       aTACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACtt                                                                                         >  1:145993/1‑130 (MQ=255)                                                             tgttAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGc                                                                         <  2:326653/140‑1 (MQ=255)                                                                   aaaTCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGATAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCAtt                                                                   <  2:295399/140‑1 (MQ=255)                                                                     aTCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCa                                                                 >  2:53249/1‑140 (MQ=255)                                                                          tGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTg                                                            <  1:279732/140‑1 (MQ=255)                                                                            tATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGaa                                                          <  1:311587/140‑1 (MQ=255)                                                                                aCCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCAtt                                                                   >  1:251563/1‑127 (MQ=255)                                                                                aCCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCAtt                                                                   <  2:251563/127‑1 (MQ=255)                                                                                    ttGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATACAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGaacaac                                                      <  2:18968/136‑1 (MQ=255)                                                                                    ttGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATACAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGaacaac                                                      >  1:18968/1‑136 (MQ=255)                                                                                     tGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGAACAACCTGGc                                                 <  2:305592/140‑1 (MQ=255)                                                                                      ggATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGAACAACCTGGCt                                                >  2:329486/1‑140 (MQ=255)                                                                                                      ccTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGAACAACCTGGCTTTATCAATAAAGTccc                                >  2:212239/1‑140 (MQ=255)                                                                                                                    cAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGAACAACCTGGCTTTATCAATAAAGTCCCGTATAACTTCCTgg                  <  2:67868/140‑1 (MQ=255)                                                                                                                            aGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGAACAACCTGGCTTTATCAATAAAGTCCCGTATAACTTCCTGGAAGTGAat          >  1:36391/1‑140 (MQ=255)                                                                                                                               gCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGAACAACCTGGCTTTATCAATAAAGTCCCGTATAACTTCCTGGAAGTGAATATg       <  2:251544/140‑1 (MQ=255)                                                                                                                                  gACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGAACAACCTGGCTTTATCAATAAAGTCCCGTATAACTTCCTGGAAGTGAATATGCAg    <  1:60160/140‑1 (MQ=255)                                                                                                                                   aCTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGAACAACCTGGCTTTATCAATAAAGTCCCGTATAACTTCCTGGAAGTGAATATGCAgg   >  2:37900/1‑140 (MQ=255)                                                                                                                                    cTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGAACAACCTGGCTTTATCAATAAAGTCCCGTATAACTTCCTGGAAGTGAATATGCAggg  >  1:269667/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       GTCAACAAGCGCGCTTTGGTGGGAAGGTTATCAACGTTATCAATGGCAAAACGGATACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTCGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGAACAACCTGGCTTTATCAATAAAGTCCCGTATAACTTCCTGGAAGTGAATATGCAGGG  >  NC_000913/3654109‑3654379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑