Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,197,698	A→G	F180F (TTT→TTC)	ymfE ←	e14 prophage; uncharacterized protein YmfE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,197,698	0	A	G	100.0%	97.0 / NA	27	F180F (TTT→TTC)	ymfE	e14 prophage; uncharacterized protein YmfE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/11);  total (16/11)

TTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAAT  >  NC_000913/1197563‑1197826                                                                                                                                        |                                                                                                                                 ttttattttaTCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGgaaaa                                                                                                                              >  2:59199/1‑140 (MQ=255)                       tttCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCaaaaaaa                                                                                                        <  2:35815/140‑1 (MQ=255)                                            cTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTaa                                                                                   >  1:195258/1‑140 (MQ=255)                                               ggCTTAACTGTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGc                                                                                >  2:78477/1‑140 (MQ=255)                                                  ttAACTGTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAAt                                                                             >  2:63433/1‑140 (MQ=255)                                                    aaCTGTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTa                                                                           >  2:191500/1‑140 (MQ=255)                                                    aaCTGTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTa                                                                           >  2:172830/1‑140 (MQ=255)                                                            aagcaaagATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAAt                                                                   >  1:82366/1‑140 (MQ=255)                                                                    ataataCCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCaaaaaa                                                           <  2:30347/140‑1 (MQ=255)                                                                           cactcactGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCaa                                                    <  2:194652/140‑1 (MQ=255)                                                                             ctcactGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaa                                                  >  2:107524/1‑140 (MQ=255)                                                                             ctcactGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaa                                                  >  1:212600/1‑140 (MQ=255)                                                                              tcactGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaaa                                                 <  1:172830/140‑1 (MQ=255)                                                                              tcactGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaaa                                                 >  1:198845/1‑140 (MQ=255)                                                                              tcactGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaaa                                                 >  1:279290/1‑140 (MQ=255)                                                                              tcactGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaaa                                                 >  2:314886/1‑140 (MQ=255)                                                                              tcactGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaaa                                                 >  2:247641/1‑140 (MQ=255)                                                                                        tGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACtt                                       <  2:198845/140‑1 (MQ=255)                                                                                        tGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACtt                                       <  1:177330/140‑1 (MQ=255)                                                                                        tGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACtt                                       <  1:334073/140‑1 (MQ=255)                                                                                                      aaaaGAATAAAGACAGCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGt                         <  1:230876/140‑1 (MQ=255)                                                                                                       aaaGAATAAAGACAGCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGtt                        <  1:248667/140‑1 (MQ=255)                                                                                                           aaTAAAGACAGCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAat                    >  1:163160/1‑140 (MQ=255)                                                                                                                  aCAGCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGt                         >  1:32265/1‑128 (MQ=255)                                                                                                                  aCAGCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGt                         <  2:32265/128‑1 (MQ=255)                                                                                                                     gCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTg          >  2:176973/1‑140 (MQ=255)                                                                                                                     gCAAAGAACCTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTg          >  1:210587/1‑140 (MQ=255)                                                                                                                             ccTTCAACATGGAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAAt  <  1:314886/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                 TTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAAT  >  NC_000913/1197563‑1197826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑