Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,359,040	C→A	L371L (CTG→CTT)	yfaV ←	putative transporter YfaV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,359,040	0	C	A	100.0%	59.3 / NA	18	L371L (CTG→CTT)	yfaV	putative transporter YfaV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (9/9);  total (9/9)

ACGCAGAGTGAAAATAATCAGCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTA  >  NC_000913/2358905‑2359167                                                                                                                                        |                                                                                                                                acGCAGAGTGAAAATAATCAGCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGt                                                                                                                             >  1:96536/1‑140 (MQ=255)                   cAGCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCg                                                                                                           >  2:137575/1‑140 (MQ=255)                     gCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCGca                                                                                                         >  2:107282/1‑140 (MQ=255)                       aacccGATGCCCGCCACCGCTCCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCGcagc                                                                                                       <  2:234887/137‑1 (MQ=255)                         aCCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTccc                                                                                                            >  2:300740/1‑133 (MQ=255)                         aCCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTccc                                                                                                            <  1:300740/133‑1 (MQ=255)                             aTGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCGCAGCCAgcgc                                                                                                 <  1:326900/140‑1 (MQ=255)                               gACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCg                                                                                               <  1:137575/140‑1 (MQ=255)                                       ccGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGaa                                                                                       <  2:188399/140‑1 (MQ=255)                                                                                                cgcgCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCt                              >  1:317781/1‑140 (MQ=255)                                                                                                    cAGGATCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCaa                          >  1:219900/1‑140 (MQ=255)                                                                                                        aTCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAacaca                      <  2:96536/140‑1 (MQ=255)                                                                                                        aTCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAacaca                      <  2:219900/140‑1 (MQ=255)                                                                                                             ggCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAgcg                 >  1:247902/1‑140 (MQ=255)                                                                                                                  tAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACg            >  1:175890/1‑140 (MQ=255)                                                                                                                     aCCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATc         <  2:175890/140‑1 (MQ=255)                                                                                                                           ccACTGCACCAAAAAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCAtt   >  1:9038/1‑140 (MQ=255)                                                                                                                            cACTGCACCAAAAAGGTTTACAAAACCAATTCCCGCAGCCAGCGCAGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTa  <  2:6525/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                ACGCAGAGTGAAAATAATCAGCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTA  >  NC_000913/2358905‑2359167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑