Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	697,054	(A)7→8	intergenic (‑7/+3)	leuW ← / ← metT	tRNA‑Leu/tRNA‑Met

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	697,047	1	.	A	95.2%	65.8 / ‑3.1	21	noncoding (1/85 nt)	leuW	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base A (9/11);  total (10/11)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

CCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGC‑AAAAAAAGATGGTGGCTA‑CGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTAT  >  NC_000913/696917‑697178                                                                                                                                    |                                                                                                                                     ccTTCCCGCTTGGCGATCCCCCATCCGTACACCGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGC‑aaaaaaaag                                                                                                                             <  2:165371/140‑3 (MQ=255)                aTACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCACAAAAAAAAGATGGTGGCTa‑cgacg                                                                                                              >  2:38794/1‑140 (MQ=255)                            tACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAAGATGGTGGCTA‑CGACGGGATTCGAACCt                                                                                                  >  2:30452/1‑140 (MQ=255)                            tACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAAGATGGTGGCTA‑CGACGGGATTCGAACCt                                                                                                  >  2:218621/1‑140 (MQ=255)                               aaCGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGC‑AAAAAAAGGTGGGGGCCACCGAC‑GGATTCGAACCtgtgg                                                                                              >  2:125143/1‑139 (MQ=255)                                  gCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAAGATGGTGGCTA‑CGACGGGATTCGAACCTGTGAcc                                                                                            <  1:218621/140‑1 (MQ=255)                                   cTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAAGATGGTGGCTA‑CGACGGGATTCGAACCTGTGAccc                                                                                           >  1:212614/1‑140 (MQ=255)                                    tttCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAAGATGGTGGCTA‑CGACGGGATTCGAACCTGTGAcccc                                                                                          >  1:318670/1‑140 (MQ=255)                                    tttCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAAGATGGTGGCTA‑CGACGGGATTCGAACCTGTGAcccc                                                                                          <  1:77446/140‑1 (MQ=255)                                     ttCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAAGATGGTGGCTA‑CGACGGGATTCGAACCTGTGACCCca                                                                                         <  2:212614/140‑1 (MQ=255)                                         ggTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAAGATGGTGGCTA‑CGACGGGATTCGAACCTGTGACCCcatcat                                                                                     <  1:2150/140‑1 (MQ=255)                                             aaTGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAAGATGGTGGCTA‑CGACGGGATTCGAACCTGTGACCCCATCATTATg                                                                                 >  1:144004/1‑140 (MQ=255)                                                   gCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAAGATGGTGGCTA‑CGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGAt                                                                           <  2:83963/140‑1 (MQ=255)                                                      ggAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAAGATGGTGGCTA‑CGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGAtgtg                                                                        <  2:318670/140‑1 (MQ=255)                                                                cTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAAGATGGTGGCTA‑CGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCaa                                                              <  2:144004/140‑1 (MQ=255)                                                                cTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAAGATGGTGGCTA‑CGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCaa                                                              <  1:30452/140‑1 (MQ=255)                                                                     aCTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCACAAAAAAAAGATGGTGGCTA‑CGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAg                                                         <  1:38794/140‑1 (MQ=255)                                                                          cacaCCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAAGATGGTGGCTA‑CGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACg                                                    >  2:231482/1‑140 (MQ=255)                                                                                                                        cGCCACTCCCGCAAAAAAAAGATGGTGGCTA‑CGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCgg            >  2:272274/1‑134 (MQ=255)                                                                                                                        cGCCACTCCCGCAAAAAAAAGATGGTGGCTA‑CGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCgg            <  1:272274/134‑1 (MQ=255)                                                                                                                        cGCCACTCCCGCAAAAAAAAGATGGTGGCTA‑CGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCa      >  2:281885/1‑140 (MQ=255)                                                                                                                            aCTCCCGCAAAAAAAAGATGGTGGCTA‑CGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTAt  <  1:281885/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                     CCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGC‑AAAAAAAGATGGTGGCTA‑CGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTAT  >  NC_000913/696917‑697178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑