Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,566,867	G→T	H92N (CAT→AAT)	dosC ←	diguanylate cyclase DosC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,566,867	0	G	T	100.0%	33.3 / NA	11	H92N (CAT→AAT)	dosC	diguanylate cyclase DosC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (1/10);  total (1/10)

ACCTGAAGTTTTTCTGCGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCAT  >  NC_000913/1566734‑1566968                                                                                                                                      |                                                                                                      aCCTGAAGTTTTTCTGCGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATTCTGGAt                                                                                                 <  1:214613/140‑1 (MQ=255) aCCTGAAGTTTTTCTGCGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATTCTGGAt                                                                                                 <  2:195348/140‑1 (MQ=255) aCCTGAAGTTTTTCTGCGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATTCTGGAt                                                                                                 <  2:65623/140‑1 (MQ=255)        gTTTTTCTGCGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATTCTGGATTTGTAtt                                                                                          <  2:278109/140‑1 (MQ=255)                       aataatCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATTCTGGATTTGTATTAGCCTTTCGACATCg                                                                           <  2:307593/140‑1 (MQ=255)                                 agagaagaTCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATTCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTggg                                                                 <  1:121441/140‑1 (MQ=255)                                 agagaagaTCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATTCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTggg                                                                 >  1:155395/1‑140 (MQ=255)                                           aCCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATTCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCAc                                                       <  1:200451/140‑1 (MQ=255)                                                            tttCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATTCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGtt                                      <  2:182552/140‑1 (MQ=255)                                                                                   cGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATTCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGc               <  1:255791/140‑1 (MQ=255)                                                                                                cGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATTCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCAt  <  2:6364/140‑1 (MQ=255)                                                                                                                                      |                                                                                                      ACCTGAAGTTTTTCTGCGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCAT  >  NC_000913/1566734‑1566968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑