Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,615,629	G→T	E276* (GAG→TAG)	yneJ →	putative LysR‑type DNA‑binding transcriptional regulator YneJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,615,629	0	G	T	100.0%	138.9 / NA	41	E276* (GAG→TAG)	yneJ	putative LysR‑type DNA‑binding transcriptional regulator YneJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (22/19);  total (22/19)

TGCGCTTATTCCGCGCTCTATGCTGGAAAGTATGCCGGGGCATCACCAGGTTGAAGCGTGGCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCGAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTGATAGTTCTTGCGGTTAATATGCTCTATATAGTGATGTTCCGATGACTTATGACTATATGGGGCAAAT  >  NC_000913/1615496‑1615762                                                                                                                                      |                                                                                                                                      tGCGCTTATTCCGCGCTCTATGCTGGAAAGTATGCCGGGGCATCACCAGGTTGAAGCGTGGCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCAt                                                                                                                                 >  2:216206/1‑140 (MQ=255)  gcgcTTATTCCGCGCTCTATGCTGGAAAGTATGCCGGGGCATCACCAGGTTGAAGCGTGGCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCAtt                                                                                                                                >  1:188763/1‑140 (MQ=255)        aTTCCGCGCTCTATGCTGGAAAGTATGCCGGGGCATCACCAGGTTGAAGCGTGGCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCa                                                                                                                          <  2:299570/140‑1 (MQ=255)              cgcTCTATGCTGGAAAGTATGCCGGGGCATCACCAGGTTGAAGCGTGGCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAActgct                                                                                                                    >  1:298609/1‑140 (MQ=255)                           aaaGTATGCCGGGGCATCACCAGGTTGAAGCGTGGCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGAc                                                                                                       >  2:219542/1‑140 (MQ=255)                             aGTATGCCGGGGCATCACCAGGTTGAAGCGTGGCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTc                                                                                                     >  2:50347/1‑140 (MQ=255)                               tATGCCGGGGCATCACCAGGTTGAAGCGTGGCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCgg                                                                                                   >  1:1308/1‑140 (MQ=255)                               tATGCCGGGGCATCACCAGGTTGAAGCGTGGCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCgg                                                                                                   >  1:57673/1‑140 (MQ=255)                               tATGCCGGGGCATCACCAGGTTGAAGCGTGGCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCgg                                                                                                   >  1:32678/1‑140 (MQ=255)                               tATGCCGGGGCATCACCAGGTTGAAGCGTGGCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCgg                                                                                                   >  1:28521/1‑140 (MQ=255)                                    cGGGGCATCACCAGGTTGAAGCGTGGCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGa                                                                                              >  2:306813/1‑140 (MQ=255)                                     ggggCATCACCAGGTTGAAGCGTGGCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGAc                                                                                             <  2:319694/140‑1 (MQ=255)                                     ggggCATCACCAGGTTGAAGCGTGGCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGAc                                                                                             <  2:195543/140‑1 (MQ=255)                                      gggCATCACCAGGTTGAAGCGTGGCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACa                                                                                            >  2:163564/1‑140 (MQ=255)                                                    tGAAGCGTGGCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAg                                                                              <  2:1308/140‑1 (MQ=255)                                                      aaGCGTGGCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCt                                                                            >  1:296172/1‑140 (MQ=255)                                                           tGGCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTAtttt                                                                       >  1:237550/1‑140 (MQ=255)                                                             gCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTg                                                                     >  1:226478/1‑140 (MQ=255)                                                             gCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTg                                                                     >  1:215224/1‑140 (MQ=255)                                                                        aGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTGATAGTTCTTGc                                                          <  1:173337/140‑1 (MQ=255)                                                                             tGGCGTTGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTGATAGTTCTTGCGGTTa                                                     >  1:161757/1‑140 (MQ=255)                                                                               gCGTTGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTGATAGTTCTTGCGGTTAat                                                   >  2:3888/1‑140 (MQ=255)                                                                                  ttGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTGATAGTTCTTGCGGTTAATATg                                                <  2:161757/140‑1 (MQ=255)                                                                                   tGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTGATAGTTCTTGCGGTTAATATGc                                               <  1:216206/140‑1 (MQ=255)                                                                                    ggTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTGATAGTTCTTGCGGTTAATATGct                                              <  2:57673/140‑1 (MQ=255)                                                                                    ggTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTGATAGTTCTTGCGGTTAATATGct                                              <  2:31104/140‑1 (MQ=255)                                                                                    ggTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTGATAGTTCTTGCGGTTAATATGct                                              <  1:3888/140‑1 (MQ=255)                                                                                     gTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTGATAGTTCTTGCGGTTAATATGctc                                             <  1:163564/140‑1 (MQ=255)                                                                                       tAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTGATAGTTCTTGCGGTTAATATGCTCta                                           >  1:31817/1‑140 (MQ=255)                                                                                              ctggctggTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTGATAGTTCTTGCGGTTAATATGCTCTATATAGTg                                    <  2:296172/140‑1 (MQ=255)                                                                                               tggctggTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTGATAGTTCTTGCGGTTAat                                                   <  1:242169/124‑1 (MQ=255)                                                                                               tggctggTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTGATAGTTCTTGCGGTTAat                                                   >  2:242169/1‑124 (MQ=255)                                                                                                 gctggTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTGATAGTTCTTGCGGTTAATATGCTCTATATAGTGATg                                 >  2:221151/1‑140 (MQ=255)                                                                                                      tCTGGCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTGATAGTTCTTGCGGTTAATATGCTCTATATAGTGATGTTCCg                            <  1:221151/140‑1 (MQ=255)                                                                                                         ggCGTCGTGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTGATAGTTCTTGCGGTTAATATGCTCTATATAGTGATGTTCCGATg                         <  2:188763/140‑1 (MQ=255)                                                                                                            gtcgtGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTGATAGTTCTTGCGGTTAATATGCTCTATATAGTGATGTTCCGATGACt                      <  2:226478/140‑1 (MQ=255)                                                                                                            gtcgtGGTGCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTGATAGTTCTTGCGGTTAATATGCTCTATATAGTGATGTTCCGATGACt                      <  1:50347/140‑1 (MQ=255)                                                                                                               gtggtgCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTGATAGTTCTTGCGGTTAATATGCTCTATATAGTGATGTTCCGATGACTTAt                   >  1:286806/1‑140 (MQ=255)                                                                                                               gtggtgCGAAAACACGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTGATAGTTCTTGCGGTTAATATGCTCTATATAGTGATGTTCCGATGACTTAt                   >  1:209751/1‑140 (MQ=255)                                                                                                                           cacGTCCGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTGATAGTTCTTGCGGTTAATATGCTCTATATAGTGATGTTCCGATGACTTATGACTATATgggg       <  2:286806/140‑1 (MQ=255)                                                                                                                                ccGCTCTAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTGATAGTTCTTGCGGTTAATATGCTCTATATAGTGATGTTCCGATGACTTATGACTATATGGGGCAAat  <  2:237550/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      TGCGCTTATTCCGCGCTCTATGCTGGAAAGTATGCCGGGGCATCACCAGGTTGAAGCGTGGCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCGTCGTGGTGCGAAAACACGTCCGCTCGAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAGACAGGGATATCAATGAGCTATTTTTGATAGTTCTTGCGGTTAATATGCTCTATATAGTGATGTTCCGATGACTTATGACTATATGGGGCAAAT  >  NC_000913/1615496‑1615762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑