Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,083,362	C→A	R203S (CGC→AGC)	sbcB →	exodeoxyribonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,083,362	0	C	A	100.0%	33.9 / NA	11	R203S (CGC→AGC)	sbcB	exodeoxyribonuclease I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (6/5);  total (6/5)

GACGGTCTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGTGTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATAAACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTGCACGTTTCCGGAATGTTTGGAGCATGGC  >  NC_000913/2083230‑2083473                                                                                                                                     |                                                                                                                gaCGGTCTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGTGTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCAAGCCTGtt                                                                                                          <  2:305539/140‑1 (MQ=255)            gAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGTGTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCAAGCCTGTTTGATTATctct                                                                                               <  2:234885/140‑1 (MQ=255)                   cGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGTGTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCAAGCCTGTTTGATTATCTCTTTACCCa                                                                                        <  1:186960/140‑1 (MQ=255)                   cGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGTGTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCAAGCCTGTTTGATTATCTCTTTACCCa                                                                                        <  2:243479/140‑1 (MQ=255)                                       aGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGTGTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCAAGCCTGTTTGATTATCTCTTTACCCATCGTAATAAACACAAACTGa                                                                    >  1:147483/1‑140 (MQ=255)                                                        aTAGCAACGCCCACGATGCGATGGCTGATGTGTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCAAGCCTGTTTGATTATCTCTTTACCCATCGTAATAAACACAAACTGATGGCGTTGATTGATGtt                                                   >  1:215952/1‑140 (MQ=255)                                                                     cgatgcgatgGCTGATGTGTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCAAGCCTGTTTGATTATCTCTTTACCCATCGTAATAAACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAAc                                      >  1:43205/1‑140 (MQ=255)                                                                                       gtACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCAAGCCTGTTTGATTATCTCTTTACCCATCGTAATAAACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTGCACGTTTCCg                    >  1:322104/1‑140 (MQ=255)                                                                                         aCGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCAAGCCTGTTTGATTATCTCTTTACCCATCGTAATAAACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTGCACGTTTCCGGa                  <  2:215952/140‑1 (MQ=255)                                                                                                       aTGGCAAAGCTGGTAAAAACGCGTCAGCCAAGCCTGTTTGATTATCTCTTTACCCATCGTAATAAACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTGCACGTTTCCGGAATGTTTGGAGCATg    >  1:289583/1‑140 (MQ=255)                                                                                                         ggCAAAGCTGGTAAAAACGCGTCAGCCAAGCCTGTTTGATTATCTCTTTACCCATCGTAATAAACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTGCACGTTTCCGGAATGTTTGGAGCATGgc  >  2:235706/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                GACGGTCTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGTGTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATAAACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTGCACGTTTCCGGAATGTTTGGAGCATGGC  >  NC_000913/2083230‑2083473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑