Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	632,233	C→A	L18M (CTG→ATG)	ybdD →	conserved protein YbdD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	632,233	0	C	A	100.0%	43.5 / NA	14	L18M (CTG→ATG)	ybdD	conserved protein YbdD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (8/6);  total (8/6)

GAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGTTTTTCCGTGAGCGGCAGGACGCGCGCTACGGTGGAAAAGGCGGC  >  NC_000913/632096‑632364                                                                                                                                          |                                                                                                                                    gAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGAtg                                                                                                                                   >  1:206142/1‑140 (MQ=255)                         ccGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGATGATGATTGGTATGCCTGATTACGAc                                                                                                           >  2:209810/1‑140 (MQ=255)                            gATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGATGATGATTGGTATGCCTGATTACGACAAc                                                                                                        >  1:223086/1‑140 (MQ=255)                              tAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGATGATGATTGGTATGCCTGATTACGACAACTa                                                                                                      <  2:118687/140‑1 (MQ=255)                                          gccgcATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGATGATGATTGGTATGCCTGATTACGACAACTATGTCGAACatat                                                                                          <  1:134980/140‑1 (MQ=255)                                          gccgcATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGATGATGATTGGTATGCCTGATTACGACAACTATGTCGAACatat                                                                                          <  1:209810/140‑1 (MQ=255)                                            cgcATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGATGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATg                                                                                         <  1:195659/139‑1 (MQ=255)                                            cgcATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGATGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATg                                                                                         >  2:195659/1‑139 (MQ=255)                                                                    aTGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGATGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGc                                                                >  2:197771/1‑140 (MQ=255)                                                                                 gCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGATGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGa                                                   >  1:186724/1‑140 (MQ=255)                                                                                                         aaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGATGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGTTTTTCCGTGAGCGGCAGGa                           >  2:236782/1‑140 (MQ=255)                                                                                                          aaGCCGGAAAATATTTAGGTCAGGCGGCGAAGATGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGTTTTTCCGTGAGCGGCAGGAc                          >  1:160571/1‑140 (MQ=255)                                                                                                          aaGCCGGAAAATATTTAGGTCAGGCGGCGAAGATGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGTTTTTCCGTGAGCGGCAGGAc                          <  1:197771/140‑1 (MQ=255)                                                                                                                                  cggcgAAGATGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGTTTTTCCGTGAGCGGCAGGACGCGCGCTACGGTGGAAAAggcggc  <  2:160571/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    GAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGTTTTTCCGTGAGCGGCAGGACGCGCGCTACGGTGGAAAAGGCGGC  >  NC_000913/632096‑632364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑