Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,166,342	G→T	intergenic (+57/‑317)	murI → / → rrsB	glutamate racemase/16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,166,342	0	G	T	100.0%	35.1 / NA	12	intergenic (+57/‑317)	murI/rrsB	glutamate racemase/16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (5/7);  total (5/7)

ACGCCAGGAGCTGAACAATTATTGCCCGTTTTACAGCGTTACGGCTTCGAAACGCTCGAAAAACTGGCAGTTTTAGGCTGATTTGGTTGAATGTTGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAACGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTGAGAACTCCGGCAGAGA  >  NC_000913/4166205‑4166433                                                                                                                                          |                                                                                            aCGCCAGGAGCTGAACAATTATTGCCCGTTTTACAGCGTTACGGCTTCGAAACGCTCGAAAAACTGGCAGTTTTAGGCTGATTTGGTTGAATGTTGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGTaa                                                                                           <  2:239794/140‑1 (MQ=255)   gCCAGGAGCTGAACAATTATTGCCCGTTTTACAGCGTTACGGCTTCGAAACGCTCGAAAAACTGGCAGTTTTAGGCTGATTTGGTTGAATGTTGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGTAATa                                                                                         <  1:324310/140‑1 (MQ=255)    ccAGGAGCTGAACAATTATTGCCCGTTTTACAGCGTTACGGCTTCGAAACGCTCGAAAAACTGGCAGTTTTAGGCTGATTTGGTTGAATGTTGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGtaataa                                                                                        <  1:117678/140‑1 (MQ=255)    ccAGGAGCTGAACAATTATTGCCCGTTTTACAGCGTTACGGCTTCGAAACGCTCGAAAAACTGGCAGTTTTAGGCTGATTTGGTTGAATGTTGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGtaataa                                                                                        <  1:180029/140‑1 (MQ=255)        gAGCTGAACAATTATTGCCCGTTTTACAGCGTTACGGCTTCGAAACGCTCGAAAAACTGGCAGTTTTAGGCTGATTTGGTTGAATGTTGCGCGGTCAGAAAAATATTTTAAATTTTCTCTTGTCAGGCCGTAATAACTcc                                                                                    <  2:256792/140‑1 (MQ=255)                   ttattGCCCGTTTTACAGCGTTACGGCTTCGAAACGCTCGAAAAACTGGCAGTTTTAGGCTGATTTGGTTGAATGTTGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGTAATAACTCCCTATAATgcgc                                                                         >  2:254976/1‑140 (MQ=255)                             ttttACAGCGTTACGGCTTCGAAACGCTCGAAAAACTGGCAGTTTTAGGCTGATTTGGTTGAATGTTGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGTAATAACTCCCTATAATGCGCCACCACTGac                                                               >  2:141797/1‑140 (MQ=255)                                      gTTACGGCTTCGAAACGCTCGAAAAACTGGCAGTTTTAGGCTGATTTGGTTGAATGTTGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGTAATAACTCCCTATAATGCGCCACCACTGACACGGaacaa                                                      >  2:72886/1‑140 (MQ=255)                                                       cTCGAAAAACTGGCAGTTTTAGGCTGATTTGGTTGAATGTTGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGTAATAACTCCCTATAATGCGCCACCACTGACACGGAACAACGGCAAACACGccgccg                                     >  1:64175/1‑140 (MQ=255)                                                          gAAAAACTGGCAGTTTTAGGCTGATTTGGTTGAATGTTGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGTAATAACTCCCTATAATGCGCCACCACTGACACGGAACAACGGCAAACACGCCGCCGGGt                                  <  1:72886/140‑1 (MQ=255)                                                                  ggCAGTTTTAGGCTGATTTGGTTGAATGTTGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGTAATAACTCCCTATAATGCGCCACCACTGACACGGAACAACGGCAAACACGCCGCCGGGTCAGCgggg                          <  1:98491/140‑1 (MQ=255)                                                                  ggCAGTTTTAGGCTGATTTGGTTGAATGTTGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGTAATAACTCCCTATAATGCGCCACCACTGACACGGAACAACGGCAAACACGCCGCCGGGTCAGCgggg                          <  2:177147/140‑1 (MQ=255)                                                                             gCTGATTTGGTTGAATGTTGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGTAATAACTCCCTATAATGCGCCACCACTGACACGGAACAACGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTgaga               >  2:15845/1‑140 (MQ=255)                                                                                          aaTGTTGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGTAATAACTCCCTATAATGCGCCACCACTGACACGGAACAACGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTGAGAACTCCGGCAgaga  <  1:141797/140‑1 (MQ=18)                                                                                                                                          |                                                                                            ACGCCAGGAGCTGAACAATTATTGCCCGTTTTACAGCGTTACGGCTTCGAAACGCTCGAAAAACTGGCAGTTTTAGGCTGATTTGGTTGAATGTTGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAACGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTGAGAACTCCGGCAGAGA  >  NC_000913/4166205‑4166433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑