| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,296,060 | 0 | C | T | 30.4% | 70.8 / 34.2 | 46 | intergenic (+266/+376) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| Reads supporting (aligned to +/- strand): ref base C (20/12); new base T (10/4); total (30/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.02e-02 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGC > NC_000913/4295923‑4296187 | cGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAATGc > 2:21576/1‑140 (MQ=17) gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAATGcc < 2:679338/140‑1 (MQ=18) ttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAATGCCTg > 2:372206/1‑140 (MQ=17) tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAATGCCTGa > 2:825042/1‑140 (MQ=17) cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGc > 2:769709/1‑138 (MQ=32) cATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACg > 1:865433/1‑140 (MQ=32) tCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAATGCCTGATGCGACGCt > 1:269963/1‑140 (MQ=17) cGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAATGCCTGATGCGACGCTTg > 2:61849/1‑140 (MQ=18) gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAATGCCTGATGCGACGCTTGc < 1:643028/140‑1 (MQ=18) gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGc < 2:685466/140‑1 (MQ=33) cGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCtt > 1:261897/1‑140 (MQ=37) tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAATGCCTGATGCGACGCTTGCCGCGTCTTATCa > 1:267580/1‑140 (MQ=25) gATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAATGCCTGATGCGACGCTTGCCGCGTCTTATCAg > 2:10187/1‑140 (MQ=25) aTGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg < 2:32674/140‑1 (MQ=25) tGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc < 2:16170/140‑1 (MQ=25) tGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc < 2:157652/140‑1 (MQ=39) cGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatc > 2:475174/1‑138 (MQ=18) cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCa > 1:454810/1‑140 (MQ=37) ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACgaaagcta > 1:686892/1‑133 (MQ=11) gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTg > 2:350874/1‑140 (MQ=37) gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTg > 2:199187/1‑140 (MQ=37) gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTg > 2:284929/1‑140 (MQ=37) gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCACAATAGCCTGATTTAGCGTg > 1:385252/1‑140 (MQ=37) aCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCACAATAGCCTGATTTAGCGTGa < 2:385252/140‑1 (MQ=37) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAtttt < 2:154844/140‑1 (MQ=37) tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAgg > 1:3117/1‑140 (MQ=37) tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAgg > 2:593836/1‑140 (MQ=37) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGt < 2:50300/140‑1 (MQ=37) gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTc < 1:437812/140‑1 (MQ=17) aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATa > 1:876217/1‑140 (MQ=37) gCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCg > 2:778538/1‑140 (MQ=38) aTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAt > 1:16170/1‑140 (MQ=38) aTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGtttct > 2:569805/1‑138 (MQ=37) aTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAt > 2:861186/1‑140 (MQ=37) ttttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATg < 2:245443/140‑1 (MQ=37) ttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGcc < 1:353078/140‑1 (MQ=37) gTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGc > 1:718131/1‑140 (MQ=38) tAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAt < 2:246697/123‑1 (MQ=38) tAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAt > 1:246697/1‑123 (MQ=39) ggATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGAc > 2:460436/1‑140 (MQ=38) gATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACa > 2:52607/1‑140 (MQ=38) gccgcATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGc > 1:882761/1‑140 (MQ=34) gccgcATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGc < 1:452279/140‑1 (MQ=34) cgcATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGc < 1:769709/138‑1 (MQ=34) cgcATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGa < 2:718131/140‑1 (MQ=34) aTCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGc > 2:552673/1‑140 (MQ=34) | CGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑‑‑‑T‑‑‑CAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGC > NC_000913/4295923‑4296187 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |