Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,224,419	G→T	A151E (GCG→GAG)	pbpG ←	peptidoglycan DD‑endopeptidase PbpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,224,419	0	G	T	100.0%	158.4 / NA	45	A151E (GCG→GAG)	pbpG	peptidoglycan DD‑endopeptidase PbpG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (21/24);  total (21/24)

TACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTCG  >  NC_000913/2224285‑2224544                                                                                                                                       |                                                                                                                              taCTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGAtttctctttc                                                                                                                          >  2:328831/1‑140 (MQ=255)  aCTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGAtttctctttcg                                                                                                                         <  1:146233/140‑1 (MQ=255)      aaCTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGcatt                                                                                                                     >  2:806744/1‑140 (MQ=255)       aCTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGcattc                                                                                                                    >  1:507167/1‑140 (MQ=255)        cTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGcattca                                                                                                                   <  1:344037/140‑1 (MQ=255)         tGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGcattcat                                                                                                                  <  1:893328/140‑1 (MQ=255)             cGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGcc                                                                                                              <  1:328831/140‑1 (MQ=255)             cGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGcc                                                                                                              <  2:634551/140‑1 (MQ=255)               aTCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCtt                                                                                                            >  1:95977/1‑140 (MQ=255)                  aaCGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTtaat                                                                                                         <  1:543932/140‑1 (MQ=255)                     ggATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATaaa                                                                                                      >  1:726788/1‑140 (MQ=255)                      gATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGACACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAg                                                                                                     <  1:210778/140‑1 (MQ=255)                         atTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCt                                                                                                  <  1:89054/140‑1 (MQ=255)                          ttGTTTGCTGGCAATGAGCAGTTTTGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATaaa                                                                                                      >  1:455434/1‑135 (MQ=255)                          ttGTTTGCTGGCAATGAGCAGTTTTGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATaaa                                                                                                      <  2:455434/135‑1 (MQ=255)                             tttGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGt                                                                                              <  2:517179/140‑1 (MQ=255)                              ttGCTGGCAATGAGCAGTCTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTa                                                                                             <  1:444189/140‑1 (MQ=255)                                 cTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAacc                                                                                          >  2:700072/1‑140 (MQ=255)                                  tGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAacca                                                                                         >  2:632260/1‑140 (MQ=255)                                   ggCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAaccac                                                                                        <  1:834276/140‑1 (MQ=255)                                   ggCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAaccac                                                                                        >  1:872514/1‑140 (MQ=255)                                   ggCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAaccac                                                                                        <  2:95977/140‑1 (MQ=255)                                              gTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATgg                                                                             >  2:707898/1‑140 (MQ=255)                                              gTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATgg                                                                             >  1:565735/1‑140 (MQ=255)                                                             gggCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTgcc                                                              >  1:340709/1‑140 (MQ=255)                                                                  gTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCt                                                                  >  2:639999/1‑131 (MQ=255)                                                                  gTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCt                                                                  <  1:639999/131‑1 (MQ=255)                                                                   ttGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCcgcg                                                        <  1:681437/140‑1 (MQ=255)                                                                   ttGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCcgcg                                                        <  2:40879/140‑1 (MQ=255)                                                                             aTGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTg                                              >  1:201133/1‑140 (MQ=255)                                                                             aTGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTg                                              >  1:114743/1‑140 (MQ=255)                                                                                cACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAg                                           >  2:823425/1‑140 (MQ=255)                                                                                              tAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGc                             <  2:872514/140‑1 (MQ=255)                                                                                               aGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCa                            >  1:789431/1‑140 (MQ=255)                                                                                                ggTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCaa                           <  2:201133/140‑1 (MQ=255)                                                                                                   tCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAg                        >  2:747520/1‑140 (MQ=255)                                                                                                   tCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAg                        >  1:477959/1‑140 (MQ=255)                                                                                                      acaaAGCGCGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCat                     <  2:616892/140‑1 (MQ=255)                                                                                                           gcgcGTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCtt                >  1:488758/1‑140 (MQ=255)                                                                                                            cgcgTGTTGTTCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTACCCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCttt               >  1:659304/1‑140 (MQ=255)                                                                                                                    gttCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGa       <  2:565735/140‑1 (MQ=255)                                                                                                                    gttCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGa       <  1:806744/140‑1 (MQ=255)                                                                                                                    gttCATTCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGa       <  2:477959/140‑1 (MQ=255)                                                                                                                         ttCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTCg  <  2:603700/140‑1 (MQ=255)                                                                                                                         ttCCGAGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTCg  <  1:157829/140‑1 (MQ=255)                                                                                                                              aGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTCg  <  2:111081/135‑1 (MQ=255)                                                                                                                              aGCGATTTCTCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTCg  >  1:111081/1‑135 (MQ=255)                                                                                                                                       |                                                                                                                              TACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTCG  >  NC_000913/2224285‑2224544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑