Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	801,713	G→T	V319F (GTT→TTT)	ybhH →	putative isomerase YbhH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	801,713	0	G	T	97.3%	255.7 / ‑0.1	74	V319F (GTT→TTT)	ybhH	putative isomerase YbhH
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (40/32);  total (41/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCACCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGG  >  NC_000913/801579‑801848                                                                                                                                       |                                                                                                                                         aTCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCACCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCAt                                                                                                                                     <  2:587822/140‑1 (MQ=255)  tCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCACCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCAtt                                                                                                                                    <  1:18398/140‑1 (MQ=255)             cGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCACCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGaa                                                                                                                         <  2:103656/140‑1 (MQ=255)                      gTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCACCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGAt                                                                                                                >  1:659265/1‑140 (MQ=255)                      gTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCACCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGAt                                                                                                                >  1:878389/1‑140 (MQ=255)                         ctattgctattTCCAGTAGTTGTGCATTGGAAGGCACCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGcc                                                                                                             >  2:321538/1‑140 (MQ=255)                              gctattTCCAGTAGTTGTGCATTGGAAGGCACCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCacgac                                                                                                        >  2:898870/1‑140 (MQ=255)                                    tCCAGTAGTTGTGCATTGGAAGGCACCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTAcg                                                                                                  >  2:266224/1‑140 (MQ=255)                                       agtagtTGTGCATTGGAAGGCACCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACgcgc                                                                                               <  1:119932/140‑1 (MQ=255)                                          agtTGTGCATTGGAAGGCACCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATc                                                                                            <  2:576265/140‑1 (MQ=255)                                          agtTGTGCATTGGAAGGCACCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATc                                                                                            <  2:648716/140‑1 (MQ=255)                                               tgCATTGGAAGGCACCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTa                                                                                       >  2:29150/1‑140 (MQ=255)                                                      gAAGGCACCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATc                                                                                            >  1:677702/1‑128 (MQ=255)                                                      gAAGGCACCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATc                                                                                            <  2:677702/128‑1 (MQ=255)                                                      gAAGGCACCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGgac                                                                                >  1:228698/1‑140 (MQ=255)                                                      gAAGGCACCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGgac                                                                                >  1:670688/1‑140 (MQ=255)                                                      gAAGGCACCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGgac                                                                                >  1:568131/1‑140 (MQ=255)                                                      gAAGGCACCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGgac                                                                                >  2:237843/1‑140 (MQ=255)                                                              cGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑aa                                                                       >  2:607696/1‑140 (MQ=255)                                                                     cGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATAtt                                                                >  2:419833/1‑140 (MQ=255)                                                                     cGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATAtt                                                                >  2:206005/1‑140 (MQ=255)                                                                           aTCGTCCCTTCTGTAGGATATGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCgg                                                          >  1:798669/1‑140 (MQ=255)                                                                           aTCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAACATTTTCCgg                                                          <  2:702980/140‑1 (MQ=255)                                                                            tCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGt                                                         <  1:898870/140‑1 (MQ=255)                                                                            tCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGt                                                         <  1:607696/140‑1 (MQ=255)                                                                                     cTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTAt                                                <  1:605385/140‑1 (MQ=255)                                                                                       gTAGGATACGGCAATATCAATATTGAACA‑CCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCtt                                             >  2:239722/1‑140 (MQ=255)                                                                                           gATACTGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTccc                                          >  2:494371/1‑140 (MQ=255)                                                                                             tACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTg                                        >  1:842051/1‑140 (MQ=255)                                                                                             tACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTg                                        >  1:168997/1‑140 (MQ=255)                                                                                               cGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGaa                                      >  1:106756/1‑140 (MQ=255)                                                                                                ggCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGaaa                                     >  1:807468/1‑140 (MQ=255)                                                                                                     tatcaatatTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAAtt                                >  1:624357/1‑140 (MQ=255)                                                                                                     tatcaatatTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAAtt                                <  1:23718/140‑1 (MQ=255)                                                                                                      atcaatatTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTc                               <  1:821057/140‑1 (MQ=255)                                                                                                          atatTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTg                           <  2:753590/140‑1 (MQ=255)                                                                                                          atatTG‑ACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGt                          <  1:297537/140‑1 (MQ=255)                                                                                                           tatTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAGAAAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTg                           >  2:592650/1‑140 (MQ=255)                                                                                                           tatTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGt                          <  2:90604/140‑1 (MQ=255)                                                                                                            atTGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTc                         <  2:505820/140‑1 (MQ=255)                                                                                                             ttGAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAGAAAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTc                         <  1:592650/140‑1 (MQ=255)                                                                                                               gAACACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAgg                      >  1:521158/1‑140 (MQ=255)                                                                                                                aaCACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGa                     >  2:587238/1‑140 (MQ=255)                                                                                                                aaCACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGa                     >  1:483396/1‑140 (MQ=255)                                                                                                                aaCACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGa                     >  2:172427/1‑140 (MQ=255)                                                                                                                aaCACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGa                     >  1:434907/1‑140 (MQ=255)                                                                                                                aaCACCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGa                     >  1:370764/1‑140 (MQ=255)                                                                                                                 acacCCCAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGAt                    <  2:878389/140‑1 (MQ=255)                                                                                                                     cccAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAgg                >  1:515870/1‑140 (MQ=255)                                                                                                                     cccAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAgg                <  1:347490/140‑1 (MQ=255)                                                                                                                      ccAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGa               >  2:812306/1‑140 (MQ=255)                                                                                                                       cAGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGAc              <  1:321538/140‑1 (MQ=255)                                                                                                                        aGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGAca             <  1:159002/140‑1 (MQ=255)                                                                                                                        aGTGGTGCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGAca             <  1:172427/140‑1 (MQ=255)                                                                                                                        aGTGGTGCGCTCGACGTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGAca             <  1:237843/140‑1 (MQ=255)                                                                                                                          tggtgCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGAcaat           <  2:670688/140‑1 (MQ=255)                                                                                                                           ggtgCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGAcaatc          <  2:509121/140‑1 (MQ=255)                                                                                                                             tgCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGAcaatcaa        <  2:180055/140‑1 (MQ=255)                                                                                                                             tgCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGAcaatcaa        <  2:569623/140‑1 (MQ=255)                                                                                                                             tgCGCTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGAcaatcaa        <  1:126894/140‑1 (MQ=255)                                                                                                                               cgcTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATa      >  2:896608/1‑140 (MQ=255)                                                                                                                               cgcTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATa      >  1:728071/1‑140 (MQ=255)                                                                                                                               cgcTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATa      >  1:288494/1‑140 (MQ=255)                                                                                                                               cgcTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATa      >  2:421087/1‑140 (MQ=255)                                                                                                                               cgcTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATa      >  1:308704/1‑140 (MQ=255)                                                                                                                               cgcTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATa      >  2:387586/1‑140 (MQ=255)                                                                                                                               cgcTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATa      >  1:41673/1‑140 (MQ=255)                                                                                                                               cgcTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATa      >  1:492801/1‑140 (MQ=255)                                                                                                                               cgcTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTGACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATa      >  2:696639/1‑140 (MQ=255)                                                                                                                                 cTCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATaaa    >  2:100789/1‑140 (MQ=255)                                                                                                                                  tCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAg   <  1:132906/140‑1 (MQ=255)                                                                                                                                  tCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAg   <  1:812306/140‑1 (MQ=255)                                                                                                                                  tCGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAg   <  1:375841/140‑1 (MQ=255)                                                                                                                                   cGACTTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAgg  <  1:29150/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         ATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCACCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGACGACCAG‑AAAAATATTTTCCGGTGAAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGG  >  NC_000913/801579‑801848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑