Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,775,691	C→A	G35G (GGC→GGA)	ydiF →	putative acetate‑CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,775,691	0	C	A	100.0%	169.1 / NA	49	G35G (GGC→GGA)	ydiF	putative acetate‑CoA transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (25/24);  total (25/24)

ACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGCGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAACACCACGTAATTTATCGATTATTAGTCCAACAGGGCTTGGCGATCGCGCCGACCGTGGTATTAGTC  >  NC_000913/1775565‑1775827                                                                                                                               |                                                                                                                                         acaTCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTa                                                                                                                             >  2:586985/1‑140 (MQ=255)             ggAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGcca                                                                                                                 <  2:777509/140‑1 (MQ=255)             ggAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGcca                                                                                                                 >  1:455096/1‑140 (MQ=255)             ggAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGcca                                                                                                                 >  1:777509/1‑140 (MQ=255)                  tAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACg                                                                                                            >  1:808546/1‑140 (MQ=255)                    aCAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGtt                                                                                                          <  1:778076/140‑1 (MQ=255)                        tGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGC‑GGCGGCGGTATTCTGGAAGCCACCACGTTAATTa                                                                                                     >  1:436992/1‑140 (MQ=255)                                gTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGctct                                                                                              >  2:123772/1‑140 (MQ=255)                                  aaaaCCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTg                                                                                            <  1:15960/140‑1 (MQ=255)                                  aaaaCCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTg                                                                                            <  2:494942/140‑1 (MQ=255)                                  aaaaCCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTg                                                                                            >  1:839405/1‑140 (MQ=255)                                      ccaccTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGa                                                                                        <  1:123772/140‑1 (MQ=255)                                      ccaccTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGa                                                                                        <  2:906700/140‑1 (MQ=255)                                      ccaccTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGa                                                                                        <  2:402549/140‑1 (MQ=255)                                        accTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATa                                                                                      >  2:28108/1‑140 (MQ=255)                                        accTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATa                                                                                      <  2:750981/140‑1 (MQ=255)                                           tCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGa                                                                                        <  2:507/135‑1 (MQ=255)                                           tCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGa                                                                                        >  1:507/1‑135 (MQ=255)                                           tCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGa                                                                                        <  2:869677/135‑1 (MQ=255)                                           tCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGa                                                                                        >  1:869677/1‑135 (MQ=255)                                           tCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAat                                                                                   >  2:751704/1‑140 (MQ=255)                                                  aaTGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACa                                                                            <  2:72318/140‑1 (MQ=255)                                                    tGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGa                                                                          >  2:132107/1‑140 (MQ=255)                                                        cGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCa                                                                      >  2:704454/1‑140 (MQ=255)                                                        cGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCa                                                                      >  1:902189/1‑140 (MQ=255)                                                        cGAGTGCCGGTCCTGTCGGCACAGGAAGCGGCGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGAATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCa                                                                      >  2:232753/1‑140 (MQ=255)                                                           gTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAac                                                                   <  1:515291/140‑1 (MQ=255)                                                                 gTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAACACCACg                                                             >  1:26380/1‑140 (MQ=255)                                                                    cTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAACACCACGTaa                                                          <  2:748971/140‑1 (MQ=255)                                                                     tGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAACACCACGTAAt                                                         <  2:403210/140‑1 (MQ=255)                                                                      gTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAACACCACGTAAtt                                                        <  1:173821/140‑1 (MQ=255)                                                                           cacaGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAACACCACGTAATTTATCg                                                   <  1:28108/140‑1 (MQ=255)                                                                            acaGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAACACCACGTAATTTATCGa                                                  >  2:638417/1‑140 (MQ=255)                                                                             caGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGC‑GGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAACACCACGTAATTTATCGatt                                                <  2:436992/140‑1 (MQ=255)                                                                                gAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAACACCACGTAATTTATCGattat                                              <  1:14622/140‑1 (MQ=255)                                                                                   gCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAACACCACGTAATTTATCGATTATTAg                                           <  1:638417/140‑1 (MQ=255)                                                                                   gCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAACACCACGTAATTTATCGATTATTAg                                           <  2:649853/140‑1 (MQ=255)                                                                                                 tCCCGACGAAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAACACCACGTAATTTATCGATTATTAGTCCAACAGGGCTTg                             >  2:562499/1‑140 (MQ=255)                                                                                                    cgacgaAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAACACCACGTAATTTATCGATTATTAGTCCAACAGGGCTTGGCg                          <  1:232753/140‑1 (MQ=255)                                                                                                     gacgaAGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAACACCACGTAATTTATCGATTATTAGTCCAACAGGGCTTGGCGa                         >  1:530342/1‑140 (MQ=255)                                                                                                         aaGCAACACTTTGTGTGTTAGGAGCTGGCGGCGGAATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAACACCACGTAATTTATCGATTATTAGTCCAACAGGGCTTGGCGATcgc                     >  2:665111/1‑140 (MQ=255)                                                                                                                acTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAACACCACGTAATTTATCGATTATTAGTCCAACAGGGCTTGGCGATCGCGCCGAcc              <  2:808546/140‑1 (MQ=255)                                                                                                                 cTTTGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAACACCACGTAATTTATCGATTATTAGTCCAACAGGGCTTGGCGATCGCGCCGACCg             <  2:130702/140‑1 (MQ=255)                                                                                                                  tttGTGTGTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAACACCACGTAATTTATCGATTATTAGTCCAACAGGGCTTGGCGATCGCGCCGACCGt            >  2:243077/1‑140 (MQ=255)                                                                                                                    tgtgtgTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAACACCACGTAATTTATCGATTATTAGTCCAACAGGGCTTGGCGATCGCGCCGACCGt            >  2:376806/1‑138 (MQ=255)                                                                                                                    tgtgtgTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAACACCACGTAATTTATCGATTATTAGTCCAACAGGGCTTGGCGATCGCGCCGACCGt            <  1:376806/138‑1 (MQ=255)                                                                                                                    tgtgtgTTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAACACCACGTAATTTATCGATTATTAGTCCAACAGGGCTTGGCGATCGCGCCGACCGTgg          >  1:112797/1‑140 (MQ=255)                                                                                                                      tgtgtTAGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAACACCACGTAATTTATCGATTATTAGTCCAACAGGGCTTGGCGATCGCGCCGACCGTGGTa        >  1:247616/1‑140 (MQ=255)                                                                                                                            aGGAGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAACACCACGTAATTTATCGATTATTAGTCCAACAGGGCTTGGCGATCGCGCCGACCGTGGTATTAGTc  >  1:173303/1‑140 (MQ=255)                                                                                                                               |                                                                                                                                         ACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGCGCTGGCGGCGGTATTCTGGAAGCCACCACGTTAATTACTGCTCTTGCTGATAAATATAAACAGACTCAAACACCACGTAATTTATCGATTATTAGTCCAACAGGGCTTGGCGATCGCGCCGACCGTGGTATTAGTC  >  NC_000913/1775565‑1775827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑