Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	31,821	G→T	L335L (CTG→CTT)	carB →	carbamoyl phosphate synthetase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	31,821	0	G	T	100.0%	81.9 / NA	24	L335L (CTG→CTT)	carB	carbamoyl phosphate synthetase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (6/18);  total (6/18)

CGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTAC‑TCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATGAA  >  NC_000913/31685‑31952                                                                                                                                         |                                                                                                                                     cGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGTGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACttatg                                                                                                                                   <  2:575559/140‑1 (MQ=255)  gAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACttatga                                                                                                                                  >  1:505873/1‑140 (MQ=255)    aaaaCGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTTATGAAc                                                                                                                                <  2:404975/140‑1 (MQ=255)          gtcgtcTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTTATGAACGACATc                                                                                                                          >  1:1116594/1‑140 (MQ=255)                        aTCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTTATGAACGACATCACTGGCGGGCGTAc                                                                                                            >  1:139171/1‑140 (MQ=255)                        aTCGAAATGAACCCACGCGTGCCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTTATTAACGACATCACTGGCGGAccgaa                                                                                                            >  1:750063/1‑136 (MQ=255)                                       cgcgTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGGTACACCCTCGACGAAATTATGAACGACATCACTGGCGGGGGGACTTCCGGCCCCCtttg                                                                                             >  2:213304/1‑138 (MQ=255)                                         cgTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTTATGAACGACATCACTGGCGGACGTAC‑TCCGGCCTCCTTCGAGc                                                                                          <  2:1116594/140‑1 (MQ=255)                                         cgTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTTATGAACGACATCACTGGCGGACGTAC‑TCCGGCCTCCTTCGAGc                                                                                          <  2:1226392/140‑1 (MQ=255)                                         cgTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTTATGAACGACATCACTGGCGGACGTAC‑TCCGGCCTCCTTCGAGc                                                                                          <  2:505873/140‑1 (MQ=255)                                         cgTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTTATGAACGACATCACTGGCGGACGTAC‑TCCGGCCTCCTTCGAGc                                                                                          <  1:399072/140‑1 (MQ=255)                                                cGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTTATGAACGACATCACTGGCGGACGTAC‑TCCGGCCTCCTTCGAGCCGTCCAt                                                                                   <  1:84671/140‑1 (MQ=255)                                                   tcttcGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTTATGAACGACATCACTGGCGGACGTAC‑TCCGGCCTCCTTCGAGCCGTCCATCGa                                                                                <  2:622155/140‑1 (MQ=255)                                                    cttcGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTTATGAACGACATCACTGGCGGACGTAC‑TCCGGCCTCCTTCGAGCCGTCCATCGAc                                                                               <  1:1022065/140‑1 (MQ=255)                                                       cGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTTATGAACGACATCACTGGCGGACGTAC‑TCCGGCCTCCTTCGAGCCGTCCATCGACTAt                                                                            <  2:1317849/140‑1 (MQ=255)                                                                       aGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTTATGAACGACATCACTGGCGGACGTAC‑TCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTc                                                            <  2:969347/140‑1 (MQ=255)                                                                                                gTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTTATGAACGACATCACTGGCGGACGTAC‑TCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGc                                   <  1:1073834/140‑1 (MQ=255)                                                                                                 tGGCGGCGAAACTGGCGG‑GGGTTACACCCTCGACGAACTTATGAACGACATCACTGGCGGACGTAC‑TCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCg                                 <  1:1156006/140‑1 (MQ=255)                                                                                                  ggcggcGAAACTGGCGGTGGGTTACACCCTCGACGAACTTATGAACGACATCACTGGCGGACGTAC‑TCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCg                                 <  1:213304/140‑1 (MQ=255)                                                                                                             tGGCGGTGGGTTACACCCTCGACGAACTTATGAACGACATCACTGGCGGTCGTAC‑TCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGAc                      <  1:276757/140‑1 (MQ=255)                                                                                                               gCGGTGGGTTACACCCTCGACGAACTTATGAACGACATCACTGGCGGACGTAC‑TCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCg                    <  1:630910/140‑1 (MQ=255)                                                                                                                         acacCCTCGACGAACTTATGAACGACATCACTGGCGGACGTAC‑TCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACt          >  1:296495/1‑140 (MQ=255)                                                                                                                          cacCCTCGACGAACTTATGAACGACATCACTGGCGGACGTAC‑TCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTc         <  2:1021799/140‑1 (MQ=255)                                                                                                                             ccTCGACGAACTTATGAACGACATCACTGGCGGACGTAC‑TCCGGcctc                                                                                                  >  1:762586/1‑48 (MQ=255)                                                                                                                             ccTCGACGAACTTATGAACGACATCACTGGCGGACGTAC‑TCCGGcctc                                                                                                  <  2:762586/48‑1 (MQ=255)                                                                                                                               tCGACGAACTTATGAACGACATCACTGGCGGACGTAC‑TCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATg    >  1:191233/1‑140 (MQ=255)                                                                                                                               tCGACGAACTTATGAACGACATCACTGGCGGACGTAC‑TCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATg    >  1:1214467/1‑140 (MQ=255)                                                                                                                               tCGACGAACTTATGAACGACATCACTGGCGGACGTAC‑TCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGGTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATg    >  2:868177/1‑140 (MQ=255)                                                                                                                                cgacgaACTTATGAACGACATCACTGGCGGACGTAC‑TCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATGa   >  2:1062989/1‑140 (MQ=255)                                                                                                                                 gacgaACTTATGAACGACATCACTGGCGGACGTAC‑TCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATGaa  <  1:290547/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTAC‑TCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATGAA  >  NC_000913/31685‑31952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑