Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,483,678	G→T	L159L (CTG→CTT)	yheT →	putative hydrolase YheT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,483,678	0	G	T	100.0%	229.2 / NA	65	L159L (CTG→CTT)	yheT	putative hydrolase YheT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (25/40);  total (25/40)

GAACCAAACCGTATGCACCGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTGGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCTGTAGCTATCATATGGAAAAGGGCTTTTCCCGCGTTTATCAGCGTTACTTGCTG  >  NC_000913/3483544‑3483813                                                                                                                                       |                                                                                                                                        gAACCAAACCGTATGCACCGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTg                                                                                                                                    <  1:352718/140‑1 (MQ=255)       aaCCGTATGCACCGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCt                                                                                                                              >  2:798335/1‑140 (MQ=255)           gTATGCACCGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCa                                                                                                                          <  1:996133/140‑1 (MQ=255)            tATGCACCGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCaa                                                                                                                         >  1:813038/1‑140 (MQ=255)            tATGCACCGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCaa                                                                                                                         >  1:120963/1‑140 (MQ=255)              tGCACCGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAaag                                                                                                                       <  1:116735/140‑1 (MQ=255)               gCACCGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAaagg                                                                                                                      <  2:120963/140‑1 (MQ=255)               gCACCGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAaagg                                                                                                                      <  2:1130235/140‑1 (MQ=255)                 aCCGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAaaggaa                                                                                                                    >  2:549174/1‑140 (MQ=255)                   cGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAaaggaagg                                                                                                                  <  1:553758/140‑1 (MQ=255)                   cGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAaaggaagg                                                                                                                  >  1:522091/1‑140 (MQ=255)                   cGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAaaggaagg                                                                                                                  >  2:796447/1‑140 (MQ=255)                   cGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAaaggaagg                                                                                                                  >  2:316084/1‑140 (MQ=255)                   cGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAaaggaagg                                                                                                                  >  1:1082113/1‑140 (MQ=255)                    gCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGc                                                                                                                 >  2:1025403/1‑140 (MQ=255)                    gCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGc                                                                                                                 >  1:520551/1‑140 (MQ=255)                      aTTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCaa                                                                                                               >  1:873896/1‑140 (MQ=255)                       tttACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAAt                                                                                                              <  2:841461/140‑1 (MQ=255)                         tACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGa                                                                                                            <  2:968384/140‑1 (MQ=255)                             aTTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCCGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGAtctc                                                                                                        >  2:784578/1‑140 (MQ=255)                              ttCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTcc                                                                                                       >  1:1090562/1‑140 (MQ=255)                               tCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTccc                                                                                                      >  2:512289/1‑140 (MQ=255)                                cGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCg                                                                                                     >  2:355030/1‑140 (MQ=255)                                cGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCg                                                                                                     >  1:592245/1‑140 (MQ=255)                                 gggCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCgg                                                                                                    >  1:1192447/1‑140 (MQ=255)                                  ggCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGt                                                                                                   >  2:399476/1‑140 (MQ=255)                                  ggCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGt                                                                                                   >  1:1025190/1‑140 (MQ=255)                                      aaaCCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGAt                                                                                               <  2:1090562/140‑1 (MQ=255)                                       aaCCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATg                                                                                              >  1:775880/1‑140 (MQ=255)                                            aaGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATgcggcg                                                                                         <  2:1025190/140‑1 (MQ=255)                                            aaGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATgcggcg                                                                                         <  1:399476/140‑1 (MQ=255)                                                 gCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGAt                                                                                    >  2:963498/1‑140 (MQ=255)                                                    aGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATGCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGt                                                                                 <  2:1363648/140‑1 (MQ=255)                                                      ttGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGtct                                                                               <  1:518796/140‑1 (MQ=255)                                                         gTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTg                                                                            <  1:1144161/140‑1 (MQ=255)                                                          tttttACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTgc                                                                           <  2:772944/140‑1 (MQ=255)                                                               aCGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGt                                                                      >  1:670522/1‑140 (MQ=255)                                                                    ggctgCAACGCGAATTTGGTCATGCGCCTACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGGGATTTTTTCTTCGCCGtttttt                                                                 >  2:1348694/1‑137 (MQ=255)                                                                       tgCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGTGGCGGTGATTGTCTCTGCGCCGTTTATGCTg                                                              <  1:549174/140‑1 (MQ=255)                                                                       tgCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTg                                                              <  2:670522/140‑1 (MQ=255)                                                                        gCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTgg                                                             <  1:1192567/140‑1 (MQ=255)                                                                        gCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTgg                                                             <  2:50930/140‑1 (MQ=255)                                                                         cAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGa                                                            <  2:1354232/140‑1 (MQ=255)                                                                          aaCGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGaa                                                           <  2:873896/140‑1 (MQ=255)                                                                            cgcgAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGc                                                         <  2:1456411/140‑1 (MQ=255)                                                                              cgAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCt                                                       <  1:316084/140‑1 (MQ=255)                                                                               gAATTTGGCCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCTg                                                      <  1:796615/140‑1 (MQ=255)                                                                                gaTTTGGTCATGCGCCAACGGCTGCCGTCGGGTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGTAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCTGt                                                     <  1:133477/139‑1 (MQ=255)                                                                                 aTTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCTGTa                                                    <  2:1082113/140‑1 (MQ=255)                                                                                  tttGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCTGTAg                                                   <  1:963498/140‑1 (MQ=255)                                                                                  tttGGTCATGCGCCAACGGCCGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCTGTAg                                                   <  1:784578/140‑1 (MQ=255)                                                                                    tGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCTGTAGCt                                                 <  1:522886/140‑1 (MQ=255)                                                                                     ggTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCTGTAGCTa                                                <  1:303890/140‑1 (MQ=255)                                                                                     ggTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCTGTAGCTa                                                <  1:1025403/140‑1 (MQ=255)                                                                                      gTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGGTTATGCTGGGAGCCTGTAGCTAt                                               >  1:1130644/1‑140 (MQ=255)                                                                                       tCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCTGTAGCTATc                                              <  2:1183525/140‑1 (MQ=255)                                                                                         aTGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGGGGTTTTTTCTGCGCCGTTTTTGCTGGGAGCCCGTTGCTATCat                                            >  2:522886/1‑140 (MQ=255)                                                                                          tGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCTGTAGCTATCata                                           <  2:1192447/140‑1 (MQ=255)                                                                                                    gCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCTGTAGCTATCATATGGAAAAggg                                 <  1:1335004/140‑1 (MQ=255)                                                                                                           tCGGCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGGGATTGTCTCTGCGCCGCTTTTTCTGGGAGCCTGTTGCTATCATATGGGAAAAGGCtttttc                          >  2:435763/1‑138 (MQ=255)                                                                                                              gCTATTCGCTCGGCGGTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCTGTAGCTATCATATGGAAAAGGGCTTTTCCcgc                       <  1:1089424/140‑1 (MQ=255)                                                                                                                           cggTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCTGTAGCTATCATATGGAAAAGGGCTTTTCCCGCGTTTATCAGCGtt          <  1:798335/140‑1 (MQ=255)                                                                                                                            ggTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCTGTAGCTATCATATGGAAAAGGGCTTTTCCCGCGTTTATCAGCGTTa         <  2:522091/140‑1 (MQ=255)                                                                                                                            ggTAATATGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCTGTAGCTATCATATGGAAAAGGGCTTTTCCCGCGTTTATCAGCGTTa         <  2:775880/140‑1 (MQ=255)                                                                                                                            agacagatGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCTGTAGCTATCATATGGAAAAGGGCTTTTCCCGCGTTTATCAGCGTTa         <  1:133316/134‑1 (MQ=255)                                                                                                                                   tGCTTGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCTGTAGCTATCATATGGAAAAGGGCTTTTCCCGCGTTTATCAGCGTTACTTGCTg  <  1:1222241/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        GAACCAAACCGTATGCACCGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTGGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCTGTAGCTATCATATGGAAAAGGGCTTTTCCCGCGTTTATCAGCGTTACTTGCTG  >  NC_000913/3483544‑3483813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑