Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	216,532	C→A	G158C (GGC→TGC)	yaeF ←	peptidase C92 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	216,532	0	C	A	100.0%	238.8 / NA	67	G158C (GGC→TGC)	yaeF	peptidase C92 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (30/37);  total (30/37)

TTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATC  >  NC_000913/216396‑216668                                                                                                                                         |                                                                                                                                         tttttGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCagcg                                                                                                                                       <  1:1181825/140‑1 (MQ=255) tttttGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCagcg                                                                                                                                       <  1:184279/140‑1 (MQ=255)   tttGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGAt                                                                                                                                     >  1:1127576/1‑140 (MQ=255)    ttGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATa                                                                                                                                    >  1:1302199/1‑140 (MQ=255)         tcCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTtat                                                                                                                               <  2:1013268/140‑1 (MQ=255)          ccTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTtata                                                                                                                              >  1:616129/1‑140 (MQ=255)                cccACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCt                                                                                                                        <  2:1438634/140‑1 (MQ=255)                    cacTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCg                                                                                                                          <  2:923715/134‑1 (MQ=255)                    cacTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCg                                                                                                                          >  1:923715/1‑134 (MQ=255)                     acTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCtt                                                                                                                   >  2:320176/1‑140 (MQ=255)                      cTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCttt                                                                                                                  <  2:274863/140‑1 (MQ=255)                      cTGCTTAACTGCGCTTTCGCCAGGCCGCTGAAGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCttt                                                                                                                  >  1:1422642/1‑140 (MQ=255)                         cTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCt                                                                                                                        >  1:910364/1‑131 (MQ=255)                         cTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCt                                                                                                                        <  2:910364/131‑1 (MQ=255)                         cTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGAt                                                                                                               >  1:332701/1‑140 (MQ=255)                          ttAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGAtt                                                                                                              >  2:368858/1‑140 (MQ=255)                                 cgcTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTgg                                                                                                       >  2:1368627/1‑140 (MQ=255)                                 cgcTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTgg                                                                                                       >  1:529860/1‑140 (MQ=255)                                  gcTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGc                                                                                                      <  2:643138/140‑1 (MQ=255)                                   cTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCa                                                                                                     >  1:1421543/1‑140 (MQ=255)                                   cTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCa                                                                                                     >  2:655267/1‑140 (MQ=255)                                     ttCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCaaa                                                                                                   <  2:616129/140‑1 (MQ=255)                                      tCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAAc                                                                                                  <  2:332701/140‑1 (MQ=255)                                      tCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAAc                                                                                                  >  1:1313453/1‑140 (MQ=255)                                      tCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAAc                                                                                                  <  2:742489/140‑1 (MQ=255)                                      tCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAAc                                                                                                  <  1:958270/140‑1 (MQ=255)                                      tCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAAc                                                                                                  <  2:1407798/140‑1 (MQ=255)                                        gCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAAcgc                                                                                                <  2:153483/140‑1 (MQ=255)                                           aGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGt                                                                                             <  1:984372/140‑1 (MQ=255)                                             gCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAA‑TCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCa                                                                                                     >  2:1362361/1‑129 (MQ=255)                                             gCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAA‑TCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCa                                                                                                     <  1:1362361/129‑1 (MQ=255)                                                       cACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTATGGc                                                                                 >  1:1121235/1‑140 (MQ=255)                                                             tGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCttgttg                                                                           <  1:440919/140‑1 (MQ=255)                                                              ggCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGc                                                                          <  2:134860/140‑1 (MQ=255)                                                              ggCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGc                                                                          >  2:941993/1‑140 (MQ=255)                                                                  aaaaTCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCgggg                                                                      <  1:320176/140‑1 (MQ=255)                                                                   aaaTCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGt                                                                     <  1:988302/140‑1 (MQ=255)                                                                   aaaTCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGt                                                                     <  1:1104857/140‑1 (MQ=255)                                                                   aaaTCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGt                                                                     <  2:1137587/140‑1 (MQ=255)                                                                   aaaTCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGt                                                                     <  1:456732/140‑1 (MQ=255)                                                                    aaTCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTa                                                                    <  1:1368627/140‑1 (MQ=255)                                                                    aaTCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTa                                                                    >  2:1386989/1‑140 (MQ=255)                                                                            gagaACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCgg                                                            <  2:1127576/140‑1 (MQ=255)                                                                             agaACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCggg                                                           <  1:1386989/140‑1 (MQ=255)                                                                                 cGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTc                                                       >  1:1380666/1‑140 (MQ=255)                                                                                    aTTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTa                                                    <  2:1302199/140‑1 (MQ=255)                                                                                          tGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGa                                              >  2:466086/1‑140 (MQ=255)                                                                                           gAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGaa                                             <  1:1105570/140‑1 (MQ=255)                                                                                           gAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGaa                                             <  1:466086/140‑1 (MQ=255)                                                                                              cacaTCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAg                                          >  1:891418/1‑140 (MQ=255)                                                                                                  tCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTa                                      <  2:1344244/140‑1 (MQ=255)                                                                                                   cTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTAt                                     <  1:368858/140‑1 (MQ=255)                                                                                                   cTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTAt                                     <  1:1335207/140‑1 (MQ=255)                                                                                                     ggCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCa                                   <  1:1212928/140‑1 (MQ=255)                                                                                                           tCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGc                             >  2:749293/1‑140 (MQ=255)                                                                                                           tCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGc                             >  2:380466/1‑140 (MQ=255)                                                                                                           tCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGc                             >  1:80557/1‑140 (MQ=255)                                                                                                                aTAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACt                                 <  1:435163/131‑1 (MQ=255)                                                                                                                aTAAAGGGAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACt                                 >  2:435163/1‑131 (MQ=255)                                                                                                                     gggAATAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCtt                   >  1:393579/1‑140 (MQ=255)                                                                                                                         aTAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCtttttt               <  1:655267/140‑1 (MQ=255)                                                                                                                         aTAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCtttttt               >  1:122505/1‑140 (MQ=255)                                                                                                                         aTAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCtttttt               >  2:145233/1‑140 (MQ=255)                                                                                                                          tAAATTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTa              <  2:221655/140‑1 (MQ=255)                                                                                                                           aaaTTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTaa             <  2:1313453/140‑1 (MQ=255)                                                                                                                           aaaTTCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTATGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTaa             <  2:1121235/140‑1 (MQ=255)                                                                                                                              ttCGACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAggg          <  2:529860/140‑1 (MQ=255)                                                                                                                                 gACAATGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGaaa       >  2:1042673/1‑140 (MQ=255)                                                                                                                                      tGCAGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATc  >  1:1442538/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         TTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATC  >  NC_000913/216396‑216668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑