BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,662,033	T→G	E33D (GAA→GAC)	iscR ←	DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,662,033	0	T	G	100.0%	402.5 / NA	107	E33D (GAA→GAC)	iscR	DNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (62/45); total (62/45)

Only 100 of 107 total aligned reads displayed.

TTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTT  >  NC_000913/2661898‑2662168

ttCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCgg                                                                                                                                     >  1:721493/1‑140 (MQ=255)

  cGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGaa                                                                                                                                   >  2:820994/1‑140 (MQ=255)

  cGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGaa                                                                                                                                   >  2:2584633/1‑140 (MQ=255)

  cGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGaa                                                                                                                                   >  2:1169779/1‑140 (MQ=255)

     cAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAACCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAata                                                                                                                                >  1:2157786/1‑140 (MQ=255)

     cAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAata                                                                                                                                >  2:857024/1‑140 (MQ=255)

      aaCGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAatat                                                                                                                               <  2:2493200/140‑1 (MQ=255)

        cGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCa                                                                                                                             <  1:1104111/140‑1 (MQ=255)

        cGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCa                                                                                                                             <  1:857024/140‑1 (MQ=255)

        cGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCa                                                                                                                             >  2:2334297/1‑140 (MQ=255)

             aTGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGa                                                                                                                                    <  2:1119574/128‑1 (MQ=255)

             aTGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGa                                                                                                                                    >  1:1119574/1‑128 (MQ=255)

             aTGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCaa                                                                                                                        >  1:1444399/1‑140 (MQ=255)

             aTGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCaa                                                                                                                        >  1:2400710/1‑140 (MQ=255)

              tgctgcTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAAc                                                                                                                       <  1:2334297/140‑1 (MQ=255)

                        aTCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGc                                                                                                             >  1:782369/1‑140 (MQ=255)

                         tCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGcc                                                                                                            <  2:1302016/140‑1 (MQ=255)

                         tCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGcc                                                                                                            <  1:191139/140‑1 (MQ=255)

                         tCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGcc                                                                                                            <  1:396661/140‑1 (MQ=255)

                             tGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCt                                                                                                        >  1:1733587/1‑140 (MQ=255)

                                cTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCa                                                                                                     >  2:1578720/1‑140 (MQ=255)

                                cTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCa                                                                                                     >  2:382682/1‑140 (MQ=255)

                                cTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCa                                                                                                     >  1:2622505/1‑140 (MQ=255)

                                cTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCa                                                                                                     >  1:81764/1‑140 (MQ=255)

                                cTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCa                                                                                                     >  1:2134797/1‑140 (MQ=255)

                                 tAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCag                                                                                                    >  2:544566/1‑140 (MQ=255)

                                     aGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAATGGTACCAGGCCCGCTTCAGAGtt                                                                                                <  1:2165313/140‑1 (MQ=255)

                                       aTAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGa                                                                                              >  1:1841933/1‑140 (MQ=255)

                                       aTAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGa                                                                                              >  1:2317635/1‑140 (MQ=255)

                                       aTAACCACCGCCTGGTCCACGTACACTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGa                                                                                              >  1:1729020/1‑140 (MQ=255)

                                         aaCCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCTAACGGTACCGGGCCCGCTTCAGAGTTGAgc                                                                                            >  2:715058/1‑140 (MQ=255)

                                               cGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACg                                                                                      >  2:303830/1‑140 (MQ=255)

                                               cGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACg                                                                                      >  1:1253433/1‑140 (MQ=255)

                                               cGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACg                                                                                      >  1:322782/1‑140 (MQ=255)

                                                 ccTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTc                                                                                    >  2:683712/1‑140 (MQ=255)

                                                         aCGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTg                                                                            >  1:171436/1‑140 (MQ=255)

                                                         aCGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTg                                                                            >  1:2573018/1‑140 (MQ=255)

                                                         aCGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTg                                                                            >  2:1406497/1‑140 (MQ=255)

                                                          cGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGc                                                                           <  1:683712/140‑1 (MQ=255)

                                                                cTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCa                                                                                   >  1:424337/1‑126 (MQ=255)

                                                                cTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCAc                                                                     <  1:2129914/140‑1 (MQ=255)

                                                                cTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCAc                                                                     >  2:2108123/1‑140 (MQ=255)

                                                                  ggAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACgg                                                                   >  1:1994684/1‑140 (MQ=255)

                                                                    aaaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCa                                                                 <  1:2506219/140‑1 (MQ=255)

                                                                       ccAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAg                                                              <  1:190210/140‑1 (MQ=255)

                                                                       ccAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAg                                                              <  2:859541/140‑1 (MQ=255)

                                                                        cAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAgc                                                             <  2:2317635/140‑1 (MQ=255)

                                                                        cAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAgc                                                             <  2:1841933/140‑1 (MQ=255)

                                                                          gACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAgcgc                                                           >  1:192139/1‑140 (MQ=255)

                                                                           aCCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCa                                                                 >  1:2011814/1‑133 (MQ=255)

                                                                           aCCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCa                                                                 <  2:2011814/133‑1 (MQ=255)

                                                                           aCCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGcc                                                          <  1:835455/140‑1 (MQ=255)

                                                                           aCCATTTTTACGCAGACGGG‑AAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAgcg                                                            <  1:846391/137‑1 (MQ=255)

                                                                           aCCATTTTTACGCAGACGGG‑AAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAgcg                                                            >  2:846391/1‑137 (MQ=255)

                                                                               tttttACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATa                                                               >  1:469429/1‑131 (MQ=255)

                                                                               tttttACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATa                                                               <  2:469429/131‑1 (MQ=255)

                                                                                ttttACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTa                                                     <  2:436726/140‑1 (MQ=255)

                                                                                    aCGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATg                                                 <  1:668597/140‑1 (MQ=255)

                                                                                     cGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGt                                                >  2:683775/1‑140 (MQ=255)

                                                                                     cGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGt                                                <  2:220931/140‑1 (MQ=255)

                                                                                     cGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGt                                                <  2:782369/140‑1 (MQ=255)

                                                                                      gCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTc                                               <  2:2036139/140‑1 (MQ=255)

                                                                                          aCGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCCAGCATTGCGGTCGCGGCATAGCGGCCTTTAGATGTCAGtc                                           <  1:1674172/140‑1 (MQ=255)

                                                                                           cGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGtct                                          >  1:1182583/1‑140 (MQ=255)

                                                                                           cGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGtct                                          <  2:1994684/140‑1 (MQ=255)

                                                                                            gggAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGtctc                                         <  1:248994/140‑1 (MQ=255)

                                                                                             ggAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCa                                        <  2:1733587/140‑1 (MQ=255)

                                                                                             ggAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCa                                        <  1:397529/140‑1 (MQ=255)

                                                                                             ggAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCa                                        <  2:1182583/140‑1 (MQ=255)

                                                                                                      ttgttCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCGAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACt                               >  2:2582859/1‑140 (MQ=255)

                                                                                                      ttgttCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACt                               >  1:1992774/1‑140 (MQ=255)

                                                                                                      ttgttCCAGATAAGAAAGGGAAATTCCCTAACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACt                               >  1:2240942/1‑140 (MQ=255)

                                                                                                        gttCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCa                                        >  1:1500022/1‑129 (MQ=255)

                                                                                                        gttCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCa                                        <  2:1500022/129‑1 (MQ=255)

                                                                                                        gttCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTc                             >  2:2502430/1‑140 (MQ=255)

                                                                                                        gttCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTc                             >  2:2414744/1‑140 (MQ=255)

                                                                                                        gttCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTc                             >  1:2117374/1‑140 (MQ=255)

                                                                                                           ccAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCAcc                          >  1:2571297/1‑140 (MQ=255)

                                                                                                                  aGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACt                   <  2:192139/140‑1 (MQ=255)

                                                                                                                   gAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTc                  >  2:887034/1‑140 (MQ=255)

                                                                                                                   gAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTc                  >  2:317345/1‑140 (MQ=255)

                                                                                                                   gAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTc                  >  2:66065/1‑140 (MQ=255)

                                                                                                                    aaaGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGTCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACt                   >  1:1412848/1‑138 (MQ=255)

                                                                                                                    aaaGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGTCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACt                   <  2:1412848/138‑1 (MQ=255)

                                                                                                                    aaaGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCg                 >  1:1041868/1‑140 (MQ=255)

                                                                                                                       gggAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGccc              <  1:2108123/140‑1 (MQ=255)

                                                                                                                        ggAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGcccc             >  1:2246375/1‑140 (MQ=255)

                                                                                                                        ggAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGcccc             >  2:1225845/1‑140 (MQ=255)

                                                                                                                         gAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCt            >  1:1336922/1‑140 (MQ=255)

                                                                                                                         gAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCt            >  1:466422/1‑140 (MQ=255)

                                                                                                                           aaTTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGc          >  2:2355421/1‑140 (MQ=255)

                                                                                                                            aTTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGcc         <  1:1545821/140‑1 (MQ=255)

                                                                                                                            aTTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGcc         <  2:1730159/140‑1 (MQ=255)

                                                                                                                             ttCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGccc        <  1:1225845/140‑1 (MQ=255)

                                                                                                                              tCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCg       <  1:1578720/140‑1 (MQ=255)

                                                                                                                              tCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCg       <  1:66065/140‑1 (MQ=255)

                                                                                                                              tCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCg       <  2:2246375/140‑1 (MQ=255)

                                                                                                                                ccTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCATCGTCACGCATTGCGGTCACGGCATAGCGCCCTCTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCggg     <  2:2040578/140‑1 (MQ=255)

                                                                                                                                  tGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGt   >  2:822030/1‑140 (MQ=255)

                                                                                                                                  tGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGt   >  2:1419263/1‑140 (MQ=255)

TTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTT  >  NC_000913/2661898‑2662168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`