Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,321,408	G→T	L97M (CTG→ATG)	phnG ←	carbon‑phosphorus lyase core complex subunit PhnG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,321,408	0	G	T	100.0%	277.8 / NA	78	L97M (CTG→ATG)	phnG	carbon‑phosphorus lyase core complex subunit PhnG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (34/44);  total (34/44)

AAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCG  >  NC_000913/4321272‑4321543                                                                                                                                         |                                                                                                                                        aagaagTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAtcgc                                                                                                                                      <  1:1894434/140‑1 (MQ=255) aagaagTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAtcgc                                                                                                                                      <  2:1480254/140‑1 (MQ=255)    aagTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGGTGCGTCATCGCGTTAATCATCGCGCa                                                                                                                                   >  2:1927036/1‑140 (MQ=255)    aagTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCa                                                                                                                                   >  1:2158283/1‑140 (MQ=255)    aagTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACCGTCAGCGTCCAGCGGGGCAATAAGGGGTTCTGATAAGTTTTGACAGCGGCGATATTGCTGCATCAGCGCCTCAATCATCGCGCa                                                                                                                                   >  2:2258378/1‑140 (MQ=255)      gTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGc                                                                                                                                 >  2:602037/1‑140 (MQ=255)      gTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGc                                                                                                                                 >  2:161998/1‑140 (MQ=255)      gTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAAT‑AGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCg                                                                                                                                >  2:1229974/1‑140 (MQ=255)       tCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCg                                                                                                                                >  1:1441293/1‑140 (MQ=255)       tCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCg                                                                                                                                >  1:2454343/1‑140 (MQ=255)        cGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGt                                                                                                                               <  2:115379/140‑1 (MQ=255)         gACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGtt                                                                                                                              >  1:1428172/1‑140 (MQ=255)         gACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGtt                                                                                                                              <  2:730760/140‑1 (MQ=255)          aCCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTc                                                                                                                             >  2:1048136/1‑140 (MQ=255)            ccGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAAT‑AGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGc                                                                                                                          <  1:1229974/140‑1 (MQ=255)             cGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGc                                                                                                                          <  2:1428172/140‑1 (MQ=255)              ggCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCg                                                                                                                         <  2:11355/140‑1 (MQ=255)                cTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTg                                                                                                                       <  1:1927036/140‑1 (MQ=255)                   gCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTg                                                                                                                    <  1:1048136/140‑1 (MQ=255)                   gCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTg                                                                                                                    >  2:1858920/1‑140 (MQ=255)                    cGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGC‑GGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGTGTTCGGCGTGCTGtt                                                                                                                  <  1:2570944/140‑1 (MQ=255)                     gTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGtt                                                                                                                  <  1:844/140‑1 (MQ=255)                          cTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCa                                                                                                             <  2:2259557/140‑1 (MQ=255)                               gCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGccc                                                                                                        <  1:1858920/140‑1 (MQ=255)                               gCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGccc                                                                                                        <  1:2254861/140‑1 (MQ=255)                                 cTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCt                                                                                                      <  1:394423/140‑1 (MQ=255)                                     cGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCAc                                                                                                  <  1:761131/140‑1 (MQ=255)                                     cGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCAc                                                                                                  <  2:2158283/140‑1 (MQ=255)                                     cGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCAc                                                                                                  <  1:2285631/140‑1 (MQ=255)                                     cGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCAc                                                                                                  <  2:2281931/140‑1 (MQ=255)                                     cGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCAc                                                                                                  <  1:1660835/140‑1 (MQ=255)                                      gTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCAcc                                                                                                 <  1:40992/140‑1 (MQ=255)                                      gTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCAcc                                                                                                 <  1:1759942/140‑1 (MQ=255)                                       tGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCAccc                                                                                                <  2:687688/140‑1 (MQ=255)                                       tGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCAccc                                                                                                <  1:2001321/140‑1 (MQ=255)                                         cGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCt                                                                                                      >  1:203558/1‑132 (MQ=255)                                         cGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCt                                                                                                      <  2:203558/132‑1 (MQ=255)                                         cGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAg                                                                                              >  2:2546310/1‑140 (MQ=255)                                         cGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAg                                                                                              <  2:1464907/140‑1 (MQ=255)                                         cGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAg                                                                                              <  1:1312115/140‑1 (MQ=255)                                         cGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAg                                                                                              >  1:1772820/1‑140 (MQ=255)                                           gCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCt                                                                                            <  2:1095103/140‑1 (MQ=255)                                                gCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGc                                                                                       <  2:1390003/140‑1 (MQ=255)                                                  gTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGctg                                                                                     <  1:672155/140‑3 (MQ=255)                                                     ccATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGc                                                                                  <  2:2264178/140‑1 (MQ=255)                                                       aTACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGt                                                                                <  1:161998/140‑1 (MQ=255)                                                        tACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTg                                                                               >  2:1218804/1‑140 (MQ=255)                                                           ggTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCg                                                                            <  2:1284596/140‑1 (MQ=255)                                                              cAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCgtca                                                                         <  1:1310806/140‑1 (MQ=255)                                                               aGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCgtcag                                                                        <  1:35162/140‑1 (MQ=255)                                                               aGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCgtcag                                                                        >  1:2011589/1‑140 (MQ=255)                                                                 cGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCgtcagtc                                                                      >  2:38657/1‑140 (MQ=255)                                                                      aGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTg                                                                               >  2:1533840/1‑126 (MQ=255)                                                                      aGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTg                                                                               <  1:1533840/126‑1 (MQ=255)                                                                       gCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGgcgc                                                                >  1:705914/1‑140 (MQ=255)                                                                           ggCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCAcgg                                                            <  1:1218804/140‑1 (MQ=255)                                                                                tAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGgcggcg                                                       >  1:887444/1‑140 (MQ=255)                                                                                tAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGgcggcg                                                       >  2:1909145/1‑140 (MQ=255)                                                                                     gTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGt                                                  >  2:1727871/1‑140 (MQ=255)                                                                                        tCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAg                                               >  2:711921/1‑140 (MQ=255)                                                                                        tCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAg                                               <  1:711921/140‑1 (MQ=255)                                                                                         cTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGc                                              <  1:477763/140‑1 (MQ=255)                                                                                              aaGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGc                                         >  2:1534579/1‑140 (MQ=255)                                                                                                gTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGt                                       >  1:1642355/1‑140 (MQ=255)                                                                                                   ttGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGc                                    >  2:1298942/1‑140 (MQ=255)                                                                                                   ttGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGc                                    >  2:499555/1‑140 (MQ=255)                                                                                                   ttGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGc                                    >  1:168072/1‑140 (MQ=255)                                                                                                    tGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGcc                                   >  2:1287051/1‑140 (MQ=255)                                                                                                     gAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCg                                  <  2:887444/140‑1 (MQ=255)                                                                                                      aaaGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGACGCCgg                                 >  2:2384620/1‑140 (MQ=255)                                                                                                       aaGTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGc                                <  1:192320/140‑1 (MQ=255)                                                                                                         gTGGCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCaa                              >  1:1225929/1‑140 (MQ=255)                                                                                                           ggCGAGATTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCaaaa                            >  2:1423694/1‑140 (MQ=255)                                                                                                                gaTTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACg                       <  1:1287051/140‑1 (MQ=255)                                                                                                                 aTTGCTGCATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGt                      >  1:1807758/1‑140 (MQ=255)                                                                                                                        cATCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCgg               <  1:602037/140‑1 (MQ=255)                                                                                                                         aTCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGt              <  1:2384620/140‑1 (MQ=255)                                                                                                                          tCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTg             <  2:2202857/140‑1 (MQ=255)                                                                                                                          tCAGCGCGTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTg             >  1:2405966/1‑140 (MQ=255)                                                                                                                              cgcgTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTgccgc         >  1:2036429/1‑140 (MQ=255)                                                                                                                              cgcgTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTgccgc         >  2:1001645/1‑140 (MQ=255)                                                                                                                              cgcgTCAATCATCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTgccgc         <  2:1225929/140‑1 (MQ=255)                                                                                                                                     atcatcGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCcg  <  1:1727871/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        AAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCG  >  NC_000913/4321272‑4321543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑