Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,044,976 C→A M276I (ATG→ATT)  gfcE ← putative exopolysaccharide export lipoprotein GfcE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,044,9760CA100.0% 411.0 / NA 115M276I (ATG→ATT) gfcEputative exopolysaccharide export lipoprotein GfcE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (52/63);  total (52/63)

Only 100 of 116 total aligned reads displayed.

TCCTTCGCATTCAGCTGATAAATATTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCTGGCGCAAGTCGC  >  NC_000913/1044839‑1045106
                                                                                                                                         |                                                                                                                                  
tCCTTCGCATTCAGCTGATAAATATTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAAt                                                                                                                                  >  1:2124776/1‑140 (MQ=255)
tCCTTCGCATTCAGCTGATAAATATTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAAt                                                                                                                                  >  1:2588823/1‑140 (MQ=255)
  cTTCGCATTCAGCTGATAAATATTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATAc                                                                                                                                >  2:2147178/1‑140 (MQ=255)
  cTTCGCATTCAGCTGATAAATATTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATAc                                                                                                                                >  1:530210/1‑140 (MQ=255)
  cTTCGCATTCAGCTGATAAATATTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATAc                                                                                                                                >  1:1670775/1‑140 (MQ=255)
       cATTCAGCTGATAAATATTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTg                                                                                                                           <  2:1670775/140‑1 (MQ=255)
        aTTCAGCTGATAAATATTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGc                                                                                                                          <  2:1443515/140‑1 (MQ=255)
        aTTCAGCTGATAAATATTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGc                                                                                                                          <  1:1534145/140‑1 (MQ=255)
           cAGCTGATAAATATTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGt                                                                                                                       >  1:1344442/1‑140 (MQ=255)
           cAGCTGATAAATATTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGt                                                                                                                       >  2:2234900/1‑140 (MQ=255)
            aGCTGATAAATATTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTc                                                                                                                      <  1:397759/140‑1 (MQ=255)
              cTGATAAATATTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCa                                                                                                                    <  2:530210/140‑1 (MQ=255)
                 aTAAATATTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCAttt                                                                                                                 <  2:2257714/140‑1 (MQ=255)
                      tatTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGc                                                                                                            >  2:325954/1‑140 (MQ=255)
                      tatTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGc                                                                                                            >  2:1325275/1‑140 (MQ=255)
                       atTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCg                                                                                                           <  1:2060587/140‑1 (MQ=255)
                        ttGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGt                                                                                                          >  1:1631918/1‑140 (MQ=255)
                        ttGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGt                                                                                                          >  2:43554/1‑140 (MQ=255)
                        ttGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGt                                                                                                          >  2:1417003/1‑140 (MQ=255)
                         tGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTg                                                                                                         <  2:1260511/140‑1 (MQ=255)
                          ggCGATTTTTCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGc                                                                                                        <  1:1950678/140‑1 (MQ=255)
                          ggCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGc                                                                                                        <  2:460916/140‑1 (MQ=255)
                          ggCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGc                                                                                                        <  2:1189629/140‑1 (MQ=255)
                          ggCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGc                                                                                                        <  2:879782/140‑1 (MQ=255)
                          ggCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGc                                                                                                        <  1:2147178/140‑1 (MQ=255)
                          ggCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGc                                                                                                        <  1:737594/140‑1 (MQ=255)
                           gCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGct                                                                                                       >  1:677973/1‑140 (MQ=255)
                           gCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGct                                                                                                       >  2:1463913/1‑140 (MQ=255)
                           gCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGct                                                                                                       >  2:201238/1‑140 (MQ=255)
                           gCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGct                                                                                                       >  2:2559636/1‑140 (MQ=255)
                            cGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGctc                                                                                                      <  2:2124776/140‑1 (MQ=255)
                               ttttGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGt                                                                                                   <  1:240681/140‑1 (MQ=255)
                                  tGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTa                                                                                                <  1:842509/140‑1 (MQ=255)
                                   gCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTAc                                                                                               <  1:1423075/140‑1 (MQ=255)
                                    ccATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTAcc                                                                                              <  2:1742093/140‑1 (MQ=255)
                                    ccATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTAcc                                                                                              <  2:1847704/140‑1 (MQ=255)
                                     cATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTAccc                                                                                             <  2:94973/140‑1 (MQ=255)
                                        tCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTa                                                                                                <  1:2519245/134‑1 (MQ=255)
                                        tCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTa                                                                                                >  2:2519245/1‑134 (MQ=255)
                                            cTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCAc                                                                                      >  1:2251877/1‑140 (MQ=255)
                                            cTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCAc                                                                                      >  1:1598777/1‑140 (MQ=255)
                                            cTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCAc                                                                                      <  1:2234900/140‑1 (MQ=255)
                                             ttGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCAcc                                                                                     <  2:2051644/140‑1 (MQ=255)
                                                  ttGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCa                                                                                >  1:1339694/1‑140 (MQ=255)
                                                    gCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACg                                                                              <  1:831756/140‑1 (MQ=255)
                                                    gCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACg                                                                              >  1:2534376/1‑140 (MQ=255)
                                                    gCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACg                                                                              >  1:149677/1‑140 (MQ=255)
                                                     cGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGa                                                                             <  2:1138498/140‑1 (MQ=255)
                                                      gTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGaa                                                                            <  1:1258632/140‑1 (MQ=255)
                                                      gTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGaa                                                                            <  1:201622/140‑1 (MQ=255)
                                                      gTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGaa                                                                            <  2:1339694/140‑1 (MQ=255)
                                                      gTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGaa                                                                            <  2:2265185/140‑1 (MQ=255)
                                                       ttGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAg                                                                           <  2:355125/140‑1 (MQ=255)
                                                          cACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACt                                                                        <  2:231593/140‑1 (MQ=255)
                                                          cACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACt                                                                        >  1:57951/1‑140 (MQ=255)
                                                            cGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACttt                                                                      <  1:2559636/140‑1 (MQ=255)
                                                                    aaaaaTCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCGGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGatcat                                                              >  1:1999131/1‑140 (MQ=255)
                                                                      aaaTCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCg                                                            <  2:1207421/140‑1 (MQ=255)
                                                                         tCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTg                                                         >  1:1966215/1‑140 (MQ=255)
                                                                          ccccGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTgc                                                        <  2:803502/140‑1 (MQ=255)
                                                                           cccGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCTAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTgcg                                                       <  1:2237925/140‑1 (MQ=255)
                                                                           cccGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTgcg                                                       <  2:2564528/140‑1 (MQ=255)
                                                                           cccGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTgcg                                                       <  2:522900/140‑1 (MQ=255)
                                                                            ccGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTgcgc                                                      <  1:2156451/140‑1 (MQ=255)
                                                                             cGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGcgcg                                                     <  2:2251877/140‑1 (MQ=255)
                                                                              gTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCg                                                            >  1:2020688/1‑132 (MQ=255)
                                                                              gTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCg                                                            <  2:2020688/132‑1 (MQ=255)
                                                                               tCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGa                                                   <  1:691899/140‑1 (MQ=255)
                                                                                         gACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATaaataaaa                                         <  1:1325275/140‑1 (MQ=255)
                                                                                                 gATTCATCCCGTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGccc                                 <  2:2520967/140‑1 (MQ=255)
                                                                                                 gATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGccc                                 <  2:1598777/140‑1 (MQ=255)
                                                                                                     cATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGt                             <  1:43554/140‑1 (MQ=255)
                                                                                                       tACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTgc                           >  2:1790694/1‑140 (MQ=255)
                                                                                                       tACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTgc                           >  1:1747959/1‑140 (MQ=255)
                                                                                                       tACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTgc                           >  1:276703/1‑140 (MQ=255)
                                                                                                       tACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTgc                           >  1:102590/1‑140 (MQ=255)
                                                                                                        aCCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTgca                          >  1:2053364/1‑140 (MQ=255)
                                                                                                        aCCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTgca                          >  1:1999767/1‑140 (MQ=255)
                                                                                                        aCCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTgca                          >  1:491367/1‑140 (MQ=255)
                                                                                                          ccTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAatat                                      >  2:731088/1‑126 (MQ=255)
                                                                                                          ccTCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAatat                                      <  1:731088/126‑1 (MQ=255)
                                                                                                            tCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGcagcag                      >  2:651350/1‑140 (MQ=255)
                                                                                                            tCAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGcagcag                      >  2:2513960/1‑140 (MQ=255)
                                                                                                             cAGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGt                     <  1:2093294/140‑1 (MQ=255)
                                                                                                              aGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGtt                    >  1:725116/1‑140 (MQ=255)
                                                                                                              aGCGTTACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGtt                    >  1:2285560/1‑140 (MQ=255)
                                                                                                                  ttACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGtt                <  2:2534376/140‑1 (MQ=255)
                                                                                                                  ttACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGtt                <  1:1790694/140‑1 (MQ=255)
                                                                                                                  ttACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGtt                <  2:2053364/140‑1 (MQ=255)
                                                                                                                   tACCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTc               <  2:2285560/140‑1 (MQ=255)
                                                                                                                    aCCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCt              >  1:330974/1‑140 (MQ=255)
                                                                                                                    aCCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCt              >  1:1383644/1‑140 (MQ=255)
                                                                                                                    aCCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCt              >  2:2276611/1‑140 (MQ=255)
                                                                                                                    aCCCAGCGCTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCt              >  1:2518953/1‑140 (MQ=255)
                                                                                                                          cgcTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCTGGCGCa        >  1:1699274/1‑140 (MQ=255)
                                                                                                                          cgcTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCTGGCGCa        >  2:1410951/1‑140 (MQ=255)
                                                                                                                          cgcTTCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCTGGCGCa        >  2:783388/1‑140 (MQ=255)
                                                                                                                             ttCCGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCTGGCGCAAGt     <  2:124292/140‑1 (MQ=255)
                                                                                                                               ccGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCTGGCGCAAGTCg   >  2:2352773/1‑140 (MQ=255)
                                                                                                                                cGCCAGGGTAATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCTGGCGCAAGTCGc  <  1:783388/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                  
TCCTTCGCATTCAGCTGATAAATATTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAAAATCCCCGTCGCATCAGCGACATCCTGATTCATACCCTCAGCGTTACCCAGCGCTTCCGCCAGGGTCATACCGCTGCGGTCCATTTTCAGCGTGCTCTGTTTACCCACTTCACCCATCACGAAGACTTTCAGATCATCGTTGCGCGGAATAAATAAAATATCGCCCGGGTGCAGCAGTTTGTTCTGGCGCAAGTCGC  >  NC_000913/1044839‑1045106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑