Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,162,733	G→T	V32V (GTG→GTT)	lpoB →	outer membrane lipoprotein ‑ activator of MrcB activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,162,733	0	G	T	100.0%	158.7 / NA	46	V32V (GTG→GTT)	lpoB	outer membrane lipoprotein ‑ activator of MrcB activity
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (41/5);  total (41/5)

TCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTGAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCGCAGCAGCCAGGCCCAATTGAGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATT  >  NC_000913/1162602‑1162869                                                                                                                                    |                                                                                                                                         tCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAgc                                                                                                                                  <  1:74909/140‑1 (MQ=255) tCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAgc                                                                                                                                  >  1:2154696/1‑140 (MQ=255)  cAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAgcg                                                                                                                                 <  2:1349176/140‑1 (MQ=255)  cAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAgcg                                                                                                                                 <  1:1545251/140‑1 (MQ=255)     aCTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCg                                                                                                                              >  1:1037230/1‑140 (MQ=255)     aCTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCg                                                                                                                              >  2:1718094/1‑140 (MQ=255)     aCTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCg                                                                                                                              <  2:1037230/140‑1 (MQ=255)         tATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGaac                                                                                                                          >  1:31103/1‑140 (MQ=255)             tataTTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAAcc                                                                                                                      <  2:802378/140‑1 (MQ=255)               tatTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAg                                                                                                                    >  1:2040395/1‑140 (MQ=255)                    gTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAg                                                                                                               >  1:928540/1‑140 (MQ=255)                     tAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGc                                                                                                              >  1:2104556/1‑140 (MQ=255)                     tAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGc                                                                                                              >  2:2018543/1‑140 (MQ=255)                     tAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGc                                                                                                              >  2:1770723/1‑140 (MQ=255)                     tAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGc                                                                                                              >  1:2471177/1‑140 (MQ=255)                     tAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGc                                                                                                              >  1:2186630/1‑140 (MQ=255)                     tAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGc                                                                                                              >  1:2448550/1‑140 (MQ=255)                      agggggTAAATCTTGATGAAAAAAATGAGTCGCTACGCCTTGATTCCCGCGGGGGCGATTTTTCTCGCCGGGTGGGGGGGGAACCGTAACCCGCCCCCGGAAAAAAAAGTAAACCCAGCGCGGAAACACCCAGCCGAGcc                                                                                                             <  2:2494665/138‑1 (MQ=255)                       aGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCca                                                                                                            >  2:52732/1‑140 (MQ=255)                          ggTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCacaa                                                                                                         >  2:1026762/1‑140 (MQ=255)                             gAATTTTGAGAAAAAAAATGAGTCGCTACGCTTTGATTACCGCGCTGGCGTTGTTTCTCGCCGGGTGTGTGGGGCACCGTGAACCGGCCCGGGTAGAAGAAGTAAACCCAGCGCGGAAACAACCAGCCGAGCCACAACAg                                                                                                      <  2:45254/140‑1 (MQ=255)                             aattCTTGATGAAAAAAATGAGTCGCTCCGCCTTGATTCCCGCGCGGGCGATTTTCCCCGCGGGGGGTGTGGGGAAAGGTAACCCTGCCCGGAAAGAAGAATTAAACCCAGCGCGGAAACACCCAGCCGAGCCACAACAg                                                                                                      <  2:2439263/137‑1 (MQ=255)                              aaTCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGc                                                                                                     >  1:1962620/1‑140 (MQ=255)                              aaTCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGc                                                                                                     >  1:149815/1‑140 (MQ=255)                                tCTTGATGAAAAAAATGAGTCGCCGCGCCTTGATTCCCGCGCGGGCGTTTTTTCTCGCGGGGTGTGGGGGGAAACGTAACCCGCCACGGGTAGAAGAAGTTAACCCAGCGCGGAAACACCCAGCCGAGCCACAACAGCCt                                                                                                   <  2:31103/140‑1 (MQ=255)                                    gatgaCAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTcc                                                                                               >  1:697977/1‑140 (MQ=255)                                    gatgaAAAAAATGAGTCGCTACGCCTTGATTCCCGCGGGGGGGTTTTTTCCCGCGGGGTGTGGGGGGCAGCGTGACCCTGCCCGGGAAGAAGAAGTAAACCCAGCGCGGAAACAACCACCCGAGCCACAACAGCCTGTcc                                                                                               <  2:2448550/140‑1 (MQ=255)                                                gggcggCTCCGCTTTGATTCCCGCGCGGGGGTTTTTTCTCGCGGGGTGTGGGGGGCAGCGGGACCCTGCACGGGAAGAAGATGTAAACCCGGCGCGGAAACACCCGCCGGAGCCACAACAGCCTGTCCCCACAGTGCCCt                                                                                   <  2:1316780/135‑1 (MQ=255)                                                  gcccctccGCTTTGATTCCCGCGGTGGCGATTTTTCTCGCGGGGTGTGGGGGGCAACGTGACCCTGCCCCGGTAGAAAAAGTAAAACCAGCGCCGGAACACCCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCg                                                                                 <  2:2299909/133‑1 (MQ=255)                                                  gTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCg                                                                                 >  2:2435037/1‑140 (MQ=255)                                                   tCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCgg                                                                                >  2:1165667/1‑140 (MQ=255)                                                   tCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCgg                                                                                >  1:1852159/1‑140 (MQ=255)                                                                   cccGGGCGGGAGATTTTTCCCGCGGGGTGGGGGGGGCAAGGTAACCCTGCCCGGGTAAAAAAAGTAAACCCGGCGCGGAAAAACCCGGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCgca                                                                <  2:499597/139‑1 (MQ=255)                                                                     cgcgCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCGTCGGTGCCGACGATCCCgcagc                                                              >  1:622424/1‑140 (MQ=255)                                                                      gcgcTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCC‑‑CGGTGCCGACGATCCCGCagcagc                                                           >  2:2440662/1‑140 (MQ=255)                                                                        gcTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCGCagcagc                                                           >  2:631304/1‑140 (MQ=255)                                                                         cTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCGCAGCAGcc                                                          >  2:23966/1‑140 (MQ=255)                                                                          tGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCGCAGCAGCCa                                                         >  2:1978340/1‑140 (MQ=255)                                                                           ggCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCGCAGCAGCCAg                                                        >  2:1763812/1‑140 (MQ=255)                                                                              gttttttCTCGCGGGGTGTGGGGGGCAACGGAACCCTGCCCGGGTAGAAGAAGTAAACCCAGCGCGGAAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCGCAGCAGCCAGGcc                                                     <  2:2186630/136‑1 (MQ=255)                                                                                      tcGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCGCAGCAGCCAGGCCCAATTGAg                                             >  2:2380483/1‑140 (MQ=255)                                                                                      tcGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCGCAGCAGCCAGGCCCAATTGAg                                             >  1:1281719/1‑140 (MQ=255)                                                                                      tcGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAACCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCGCAGCAGCCAGGCCCAATTgct                                             >  1:2078406/1‑138 (MQ=255)                                                                                            ggTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCGCAGCAGCCAGGCCCAATTGAGCACGaa                                       >  1:1572509/1‑140 (MQ=255)                                                                                             gtgtgtGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCGCAGCAGCCAGGCCCAATTGAGCACGAAg                                      >  2:1253028/1‑140 (MQ=255)                                                                                               gtgtgGGGCAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCGCAGCAGCCAGGCCCAATTGAGCACGAAGAt                                    >  2:2180133/1‑140 (MQ=255)                                                                                                  gggggCAACGTGAACCTGCACGGGTAGAAGAAGTAAAACCAGCGCGGGAACAACCACCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCGCAGCAGCCAGGCCCAATTGAGCACGAAGATCaa                                 <  1:1026762/139‑1 (MQ=255)                                                                                                       cAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCGCAGCAGCCAGGCCCAATTGAGCACGAAGATCAAACTGc                            >  2:1304544/1‑140 (MQ=255)                                                                                                       cAACGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCGCAGCAGCCAGGCCCAATTGAGCACGAAGATCAAACTGc                            >  2:539700/1‑140 (MQ=255)                                                                                                        aaCGTGAACCTGCGCCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCGCAGCAGCCAGGCCCAATTGAGCACGAAGATCAAACTGCa                           >  2:1337982/1‑140 (MQ=255)                                                                                                        aaCGTGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCGCAGCAGCCAGGCCCAATTGAGCACGAAGATCAAACTGCa                           >  2:1297622/1‑140 (MQ=255)                                                                                                            tGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCGCAGCAACCAGGCCCAATTGAGCACGAAGATCAAACTGCACCGc                       >  2:178388/1‑140 (MQ=255)                                                                                                            tGAACCTGCACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCGCAGCAACCAGGCCCAATTGAGCACGAAGATCAAACTGCACCGc                       >  2:2420136/1‑140 (MQ=255)                                                                                                                   gccccGGAAAAAGAATTAAACCCAGCGCGGAAACACCCGGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCGCAGCAGCCAGGCCCAATTGAGCACGAAGATCAAACTGCACCGCCTGcgcc                <  1:74900/137‑1 (MQ=255)                                                                                                                    cACCGGTAGAAGAAGTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCGCAGCAGCCAGGCCCAATTGAGCACGAAGATCAAACTGCACCGCCTGcgccg               >  2:1488805/1‑140 (MQ=255)                                                                                                                                 agTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCGCAGCAGCCAGGCCCAATTGAGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCAtt  >  2:457544/1‑140 (MQ=255)                                                                                                                                 agTTAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCGCAGCAGCCAGGCCCAATTGAGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCAtt  >  2:635960/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                         TCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCACCGGTAGAAGAAGTGAAACCAGCGCCGGAACAACCAGCCGAGCCACAACAGCCTGTCCCCACAGTGCCCTCGGTGCCGACGATCCCGCAGCAGCCAGGCCCAATTGAGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATT  >  NC_000913/1162602‑1162869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑