BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,206,993	G→T	F180L (TTC→TTA)	purH ←	bifunctional AICAR transformylase/IMP cyclohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,206,993	0	G	T	100.0%	440.2 / NA	123	F180L (TTC→TTA)	purH	bifunctional AICAR transformylase/IMP cyclohydrolase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (52/71); total (52/71)

Only 100 of 125 total aligned reads displayed.

ATCCAGCTTCTTAATGAAGTTCAGGTTCAGCGTGCGTGGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCGAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAG  >  NC_000913/4206858‑4207128

atCCAGCTTCTTAATGAAGTTCAGGTTCAGCGTGCGTGGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAgg                                                                                                                                     >  1:393818/1‑140 (MQ=255)

   cAGCTTCTTAATGAAGTTCAGGTTCAGCGTGCGTGGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCtt                                                                                                                                  >  1:1320957/1‑140 (MQ=255)

    aGCTTCTTAATGAAGTTCAGGTTCAGCGTGCGTGGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCttt                                                                                                                                 >  2:1237909/1‑140 (MQ=255)

      cttcttAATGAAGTTCAGGTTCAGCGTGCGTGGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGa                                                                                                                               >  1:1511696/1‑140 (MQ=255)

         cttAATGAAGTTCAGGTTCAGCGTGCGTGGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATgg                                                                                                                            >  1:562968/1‑140 (MQ=255)

          ttAATGAAGTTCAGGTTCAGCGTGCGTGGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGc                                                                                                                           >  1:1391910/1‑140 (MQ=255)

           tAATGAAGTTCAGGTTCAGCGTGCGTGGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCg                                                                                                                          >  1:588933/1‑140 (MQ=255)

           tAATGAAGTTCAGGTTCAGCGTGCGTGGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCg                                                                                                                          >  2:899052/1‑140 (MQ=255)

           tAATGAAGTTCAGGTTCAGCGTGCGTGGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCg                                                                                                                          <  2:562968/140‑1 (MQ=255)

            aaTGAAGTTCAGGTTCAGCGTGCGTGGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGa                                                                                                                         <  2:822125/140‑1 (MQ=255)

            aaTGAAGTTCAGGTTCAGCGTGCGTGGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGa                                                                                                                         >  2:1763520/1‑140 (MQ=255)

              tGAAGTTCAGGTTCAGCGTGCGTGGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAgg                                                                                                                       <  2:979921/140‑1 (MQ=255)

                  gttcaggttcagCGTGCGTGGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGa                                                                                                                   >  2:13669/1‑140 (MQ=255)

                    tcaggttcagCGTGCGTGGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGaaa                                                                                                                 >  1:1057353/1‑140 (MQ=255)

                    tcaggttcagCGTGCGTGGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGaaa                                                                                                                 <  1:1402759/140‑1 (MQ=255)

                      aggttcagCGTGCGTGGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACg                                                                                                               >  2:773004/1‑140 (MQ=255)

                      aggttcagCGTGCGTGGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACg                                                                                                               >  2:106828/1‑140 (MQ=255)

                             gcgtgcgtGGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCa                                                                                                        >  2:520572/1‑140 (MQ=255)

                              cgtgcgtgGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCaa                                                                                                       >  1:267168/1‑140 (MQ=255)

                                  cgtgGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGt                                                                                                   <  2:271085/140‑1 (MQ=255)

                                  cgtgGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGt                                                                                                   <  2:395871/140‑1 (MQ=255)

                                  cgtgGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGt                                                                                                   >  1:680067/1‑140 (MQ=255)

                                  cgtgGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGt                                                                                                   >  1:332086/1‑140 (MQ=255)

                                      ggAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGc                                                                                               <  1:735768/140‑1 (MQ=255)

                                       gAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCg                                                                                              <  2:379385/140‑1 (MQ=255)

                                       gAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCg                                                                                              <  2:393818/140‑1 (MQ=255)

                                       gAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCg                                                                                              <  2:267168/140‑1 (MQ=255)

                                        aaGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGa                                                                                             <  1:1517015/140‑1 (MQ=255)

                                            gACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCt                                                                                         <  2:1429452/140‑1 (MQ=255)

                                            gACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCt                                                                                         <  2:1905613/140‑1 (MQ=255)

                                            gACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCt                                                                                         >  1:1429452/1‑140 (MQ=255)

                                             aCCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCtt                                                                                        <  2:1391910/140‑1 (MQ=255)

                                             aCCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCtt                                                                                        >  2:10493/1‑140 (MQ=255)

                                             aCCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCtt                                                                                        <  1:899052/140‑1 (MQ=255)

                                             aCCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCtt                                                                                        <  1:106828/140‑1 (MQ=255)

                                             aCCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCtt                                                                                        <  2:588933/140‑1 (MQ=255)

                                             aCCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCt                                                                                         >  1:385721/1‑139 (MQ=255)

                                             aCCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCt                                                                                         <  2:385721/139‑1 (MQ=255)

                                                ggcggcTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGt                                                                                     <  2:394842/140‑1 (MQ=255)

                                                ggcggcTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGt                                                                                     <  2:1320957/140‑1 (MQ=255)

                                                 gcggcTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGGAGCAGTTGGCAATCATGCTGTCGTAGGCGGCCGTGGGTTCTCAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGtt                                                                                    >  2:1482267/1‑140 (MQ=255)

                                                 gcggcTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGtt                                                                                    >  1:1772643/1‑140 (MQ=255)

                                                  cggcTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTg                                                                                   <  1:82816/140‑1 (MQ=255)

                                                          ttGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTcatccat                                                                           <  1:881347/140‑1 (MQ=255)

                                                          ttGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTcatccat                                                                           <  2:317775/140‑1 (MQ=255)

                                                             cTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCAtctc                                                                        <  1:1551919/140‑1 (MQ=255)

                                                              tttCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATctct                                                                       <  1:1763520/140‑1 (MQ=255)

                                                               ttCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTcatccat                                                                           <  1:1520209/135‑1 (MQ=255)

                                                               ttCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTcatccat                                                                           >  2:1520209/1‑135 (MQ=255)

                                                               ttCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCtt                                                                      <  1:31260/140‑1 (MQ=255)

                                                                   ccGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTtaat                                                                  >  1:973763/1‑140 (MQ=255)

                                                                     gTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTtaataa                                                                <  1:319889/140‑1 (MQ=255)

                                                                     gTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTtaataa                                                                <  1:1011598/140‑1 (MQ=255)

                                                                      tGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTaataat                                                               <  1:2009455/140‑1 (MQ=255)

                                                                      tGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTaataat                                                               <  2:2068992/140‑1 (MQ=255)

                                                                      tGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTaataat                                                               <  1:1513195/140‑1 (MQ=255)

                                                                         tAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGc                                                            >  1:512683/1‑140 (MQ=255)

                                                                          aaGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCg                                                           <  2:1511696/140‑1 (MQ=255)

                                                                            gCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTc                                                         <  2:1772643/140‑1 (MQ=255)

                                                                              cGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCAt                                                       <  2:354810/140‑1 (MQ=255)

                                                                              cGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCAt                                                       <  2:52177/140‑1 (MQ=255)

                                                                                 aaCCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGt                                                    <  2:1237881/140‑1 (MQ=255)

                                                                                 aaCCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGt                                                    >  1:1957033/1‑140 (MQ=255)

                                                                                 aaCCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGt                                                    <  2:84458/140‑1 (MQ=255)

                                                                                  aCCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTc                                                   >  2:1815906/1‑140 (MQ=255)

                                                                                   ccATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCg                                                  <  2:332086/140‑1 (MQ=255)

                                                                                   ccATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCg                                                  <  1:419254/140‑1 (MQ=255)

                                                                                   ccATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCg                                                  <  1:347988/140‑1 (MQ=255)

                                                                                   ccATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCg                                                  <  1:1915917/140‑1 (MQ=255)

                                                                                    cATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCgc                                                 <  1:10493/140‑1 (MQ=255)

                                                                                      tgctgcCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCg                                                  <  2:786847/137‑1 (MQ=255)

                                                                                      tgctgcCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCg                                                  >  1:786847/1‑137 (MQ=255)

                                                                                      tgctgcCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCAATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCg                                                  >  1:1395250/1‑137 (MQ=255)

                                                                                           ccGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCa                                                        >  1:1215226/1‑126 (MQ=255)

                                                                                           ccGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCa                                                        <  2:1215226/126‑1 (MQ=255)

                                                                                           ccGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCtt                                          >  2:1288182/1‑140 (MQ=255)

                                                                                           ccGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCtt                                          >  1:777938/1‑140 (MQ=255)

                                                                                              aaGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTcac                                       <  2:1950804/140‑1 (MQ=255)

                                                                                                  agtTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACg                                   <  1:1770239/140‑1 (MQ=255)

                                                                                                  agtTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACg                                   >  1:251653/1‑140 (MQ=255)

                                                                                                   gtTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGa                                  >  2:1595855/1‑140 (MQ=255)

                                                                                                      ggCAATCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTg                               <  2:2096370/140‑1 (MQ=255)

                                                                                                         aaTCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGa                            <  2:1774729/140‑1 (MQ=255)

                                                                                                           tCATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACa                          >  2:18090/1‑140 (MQ=255)

                                                                                                            cATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACAt                         <  2:1057353/140‑1 (MQ=255)

                                                                                                            cATGCTGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACAt                         <  2:973763/140‑1 (MQ=255)

                                                                                                                 tGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTa                    >  1:826795/1‑140 (MQ=255)

                                                                                                                 tGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTa                    <  2:826795/140‑1 (MQ=255)

                                                                                                                 tGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTa                    <  1:1232996/140‑1 (MQ=255)

                                                                                                                 tGTCGTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTa                    <  2:1858010/140‑1 (MQ=255)

                                                                                                                     gTAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGt                >  2:1978273/1‑140 (MQ=255)

                                                                                                                      tAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGtt               >  1:925933/1‑140 (MQ=255)

                                                                                                                      tAGGCGGCAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGtt               >  1:2052519/1‑140 (MQ=255)

                                                                                                                          cggcAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAACGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTg           >  2:1294986/1‑140 (MQ=255)

                                                                                                                           ggcAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTgg          >  1:2162366/1‑140 (MQ=255)

                                                                                                                             cAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACAt                         >  1:346537/1‑123 (MQ=255)

                                                                                                                             cAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACAt                         <  2:346537/123‑1 (MQ=255)

                                                                                                                             cAGTGTGTTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTggcg        >  2:252918/1‑140 (MQ=255)

                                                                                                                                tgtgtTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGgcggcg     <  2:1017773/140‑1 (MQ=255)

                                                                                                                                   gtTCTAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAg  <  1:1288182/140‑1 (MQ=255)

ATCCAGCTTCTTAATGAAGTTCAGGTTCAGCGTGCGTGGGAAGCGACCGGCGGCTTCTTTGCTTTCACCGTGGTAAGCCGGAACCATGCTGCCGAAGTAGTTGGCAATCATGCTGTCGTAGGCGGCAGTGTGTTCGAAGGCTTTGATGGCGAGGTCGAAACGGGTTGCAAGCGTCAGCGATCCTTCGTTGTCATCCATCTCTTTAATAATGGCGTCATAGTCGCTGCTCTTCACCACGATTGCGACATCTTTATGGTTCTTGGCGGCGGAG  >  NC_000913/4206858‑4207128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`