Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,549,842	C→A	G814G (GGC→GGA)	fdnG →	formate dehydrogenase N subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,549,842	0	C	A	100.0%	305.8 / NA	86	G814G (GGC→GGA)	fdnG	formate dehydrogenase N subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (51/35);  total (51/35)

GGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGCAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTA  >  NC_000913/1549706‑1549978                                                                                                                                         |                                                                                                                                          gggCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAAt                                                                                                                                        >  1:1032596/1‑140 (MQ=255)     gTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCg                                                                                                                                    <  2:2072585/140‑1 (MQ=255)       ggCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGaaa                                                                                                                                         >  2:477618/1‑133 (MQ=255)       ggCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCa                                                                                                                                  <  1:896782/140‑1 (MQ=255)       ggCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCa                                                                                                                                  <  1:513429/140‑1 (MQ=255)       ggCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCa                                                                                                                                  >  1:1356046/1‑140 (MQ=255)       ggCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCa                                                                                                                                  <  2:468557/140‑1 (MQ=255)                       cgTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCg                                                                                                                  >  2:247041/1‑140 (MQ=255)                        gTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCgg                                                                                                                 <  1:1771616/140‑1 (MQ=255)                        gTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCgg                                                                                                                 <  1:2165977/140‑1 (MQ=255)                        gTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCgg                                                                                                                 <  2:1032596/140‑1 (MQ=255)                         tGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCggg                                                                                                                <  1:1610460/140‑1 (MQ=255)                          gCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGc                                                                                                               <  1:1692612/140‑1 (MQ=255)                           cTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCg                                                                                                                  <  2:1181188/136‑1 (MQ=255)                           cTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCg                                                                                                                  >  1:1181188/1‑136 (MQ=255)                                cAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTa                                                                                                         >  1:719605/1‑140 (MQ=255)                                   ccGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACTGGGCCGTTTATCa                                                                                                      >  2:793967/1‑140 (MQ=255)                                     gTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATg                                                                                                    >  1:182610/1‑140 (MQ=255)                                     gTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATg                                                                                                    <  2:308232/140‑1 (MQ=255)                                     gTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATg                                                                                                    >  1:1170185/1‑140 (MQ=255)                                      tGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGcc                                                                                                              <  2:507911/129‑1 (MQ=255)                                      tGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGcc                                                                                                              >  1:507911/1‑129 (MQ=255)                                      tGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGc                                                                                                   <  2:1055897/140‑1 (MQ=255)                                      tGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGc                                                                                                   >  1:2142604/1‑140 (MQ=255)                                      tGCTTCGGCGGATATCAACGGTAAACCGTAGGATCCGAAACGGATGCTGAACCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAATCGGGCCGTTTATCATGc                                                                                                   >  2:1859767/1‑140 (MQ=255)                                            ggcggATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCgg                                                                                             <  2:182610/140‑1 (MQ=255)                                            ggcggATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTAGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCgg                                                                                             <  1:414192/140‑1 (MQ=255)                                             gcggATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGa                                                                                            <  1:1040488/140‑1 (MQ=255)                                             gcggATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGa                                                                                            <  2:1388013/140‑1 (MQ=255)                                                 atatCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑ggg                                                                                       >  2:521095/1‑140 (MQ=255)                                                       aCGGTAAACCGTGGGATCCGAAACGGGTGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGa                                                                                 <  2:1244019/140‑1 (MQ=255)                                                       aCGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGa                                                                                 <  1:521095/140‑1 (MQ=255)                                                        cGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGAc                                                                                >  1:1873628/1‑140 (MQ=255)                                                        cGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGAc                                                                                <  1:1869951/140‑1 (MQ=255)                                                             aaCCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCgg                                                                                             <  1:2073269/123‑1 (MQ=255)                                                             aaCCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCgg                                                                                             >  2:2073269/1‑123 (MQ=255)                                                              aCCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGt                                                                          <  2:744242/140‑1 (MQ=255)                                                              aCCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGt                                                                          >  2:1014706/1‑140 (MQ=255)                                                                   gggATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGcc                                                                     >  1:899638/1‑140 (MQ=255)                                                                    ggATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCa                                                                    >  2:1709080/1‑140 (MQ=255)                                                                     gATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGcc                                                                     >  1:408247/1‑138 (MQ=255)                                                                     gATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGcc                                                                     <  2:408247/138‑1 (MQ=255)                                                                                gATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATgg                                                        >  2:1945536/1‑140 (MQ=255)                                                                                 aTGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGc                                                       >  2:657990/1‑140 (MQ=255)                                                                                 aTGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGc                                                       >  2:1636280/1‑140 (MQ=255)                                                                                          cAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTcc                                              >  1:1710544/1‑140 (MQ=255)                                                                                          cAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTcc                                              >  2:1735629/1‑140 (MQ=255)                                                                                           aGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCg                                             >  1:1185913/1‑140 (MQ=255)                                                                                            gTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGt                                            >  1:2061790/1‑140 (MQ=255)                                                                                             tGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAAt                                                          >  1:723512/1‑125 (MQ=255)                                                                                             tGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAAt                                                          <  2:723512/125‑1 (MQ=255)                                                                                               gAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTcc                                         >  1:1560375/1‑140 (MQ=255)                                                                                               gAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTcc                                         <  2:1185913/140‑1 (MQ=255)                                                                                               gAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTcc                                         >  1:2067960/1‑140 (MQ=255)                                                                                               gAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTcc                                         <  2:899638/140‑1 (MQ=255)                                                                                                 aCGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCg                                       >  1:1302800/1‑140 (MQ=255)                                                                                                 aCGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCg                                       >  1:2151336/1‑140 (MQ=255)                                                                                                  cGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCgg                                      >  1:1600296/1‑140 (MQ=255)                                                                                                   ggCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGa                                     <  2:2142604/140‑1 (MQ=255)                                                                                                    gcagcaAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGaa                                    >  2:1779095/1‑140 (MQ=255)                                                                                                    gcagcaAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGaa                                    <  1:1779095/140‑1 (MQ=255)                                                                                                     cagcaAGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAac                                   >  1:1729598/1‑140 (MQ=255)                                                                                                          aGTGGACGGGTAACGATATTCCTGACTTCGGAAATGCCGCCCCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTAc                              <  2:1821760/140‑1 (MQ=255)                                                                                                             ggACGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAg                           >  2:2132158/1‑140 (MQ=255)                                                                                                               aCGGGTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGcc                         >  1:785353/1‑140 (MQ=255)                                                                                                                  ggTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAACCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGAt                      >  1:616706/1‑140 (MQ=255)                                                                                                                   gTAACGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGAtt                     >  1:323104/1‑140 (MQ=255)                                                                                                                      aCGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGaa                  >  1:729079/1‑140 (MQ=255)                                                                                                                       cGATATTCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGaaa                 <  2:323104/140‑1 (MQ=255)                                                                                                                            ttCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAAcgccg            <  1:2132158/140‑1 (MQ=255)                                                                                                                            ttCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAAcgccg            <  2:2061790/140‑1 (MQ=255)                                                                                                                            ttCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAAcgccg            >  2:644062/1‑140 (MQ=255)                                                                                                                             tCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAAcgccgc           >  1:751387/1‑140 (MQ=255)                                                                                                                             tCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAAcgccgc           >  2:792464/1‑140 (MQ=255)                                                                                                                             tCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAAcgccgc           >  1:810347/1‑140 (MQ=255)                                                                                                                             tCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAAcgccgc           >  2:1071628/1‑140 (MQ=255)                                                                                                                             tCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAAcgccgc           >  2:1166838/1‑140 (MQ=255)                                                                                                                             tCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAAcgccgc           >  2:1403707/1‑140 (MQ=255)                                                                                                                             tCCTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAAcgccgc           >  2:1550958/1‑140 (MQ=255)                                                                                                                              ccTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCt          <  1:647586/140‑1 (MQ=255)                                                                                                                              ccTGACTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCt          >  2:647586/1‑140 (MQ=255)                                                                                                                                  aCTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAAGGGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAAcgccg            >  1:600355/1‑135 (MQ=255)                                                                                                                                  aCTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAAGGGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAAcgccg            <  2:600355/135‑1 (MQ=255)                                                                                                                                  aCTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAAGGGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAAcgccg            <  2:1284696/135‑1 (MQ=255)                                                                                                                                  aCTTCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAAGGGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAAcgccg            >  1:1284696/1‑135 (MQ=255)                                                                                                                                    ttCGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCAc    >  1:788209/1‑140 (MQ=255)                                                                                                                                      cGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTa  >  2:1555343/1‑140 (MQ=255)                                                                                                                                      cGGAAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGAGGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCTGCTCCCGGGACACTACGAGCCGATTGAAACGCCGCTGGGCACTa  >  2:299823/1‑140 (MQ=25)                                                                                                                                         |                                                                                                                                          GGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGCAATGCCGCACCGGGTACGCCAACCGGGCCGTTTATCATGCAGCCGGAA‑GGGATGGGACGCCTGTTTGCCATCAACAAAATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTA  >  NC_000913/1549706‑1549978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑