| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,206,435 | 0 | C | T | 36.8% | 17.8 / 10.2 | 19 | intergenic (‑179/‑28) | ttdR/ttdA | DNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (0/12); new base T (7/0); total (7/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.98e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGA‑CAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTG > NC_000913/3206305‑3206561 | tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAAA‑Caaaa > 2:575726/1‑139 (MQ=255)tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGA‑Caaaa > 1:249475/1‑139 (MQ=255) cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCTGA‑CAaaaaaaaaaa > 2:44434/1‑140 (MQ=255) tGCTCTGGCGCAATATTTATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGA‑CAAAAACAAAAAATg > 1:52289/1‑140 (MQ=255) tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTTACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCCAA‑CAAAAAAAAAAAATg > 2:112820/1‑140 (MQ=255) gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGA‑CAAATACAAAAACTgg < 1:575726/140‑1 (MQ=255) ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGGATTTTTTCTGA‑CAAAAAAAAAAAATGGAGATg > 2:298915/1‑140 (MQ=255) cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGA‑CAAAAAAAAAAAATGGAGGTGCCat > 2:358238/1‑140 (MQ=255) atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTCGA‑CAAAAAAAAAAAATGAGGTTgttaaga > 1:295105/1‑134 (MQ=255) cagttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGA‑CAAATACAAAAACTGGAGTTGCCATGATGAg < 1:540336/137‑1 (MQ=255) attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGA‑CAAATACAAAAACTGGAGTTGCCATGATGAGCg < 2:252404/140‑1 (MQ=255) ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGA‑CAAATACAAAAACTGGAGTTGCCATGATGAGCGa < 1:44434/140‑1 (MQ=255) cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAA‑CAAAAAAAAAAAATGGAGTTGCCATTATGAGCGaaa > 2:112018/1‑140 (MQ=255) tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGA‑CAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat < 1:298915/140‑1 (MQ=255) gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGA‑AAAAAACAAAAAATTGAGGTGCCCTGGTGGGCGGAAAgaataatt > 2:540336/1‑138 (MQ=255) ttGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGA‑CAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAc < 1:433997/140‑1 (MQ=255) tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGA‑CAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACa < 1:358238/140‑1 (MQ=255) aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGA‑CAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa < 1:270679/140‑1 (MQ=255) ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGA‑CAAAAAAAAAAAAAGGAGGTGCCATGATGAGCGAAAGGAATAAACAACAAGCAGTGAATAAGt > 1:134379/1‑140 (MQ=255) tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGA‑CAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga < 2:398224/140‑1 (MQ=255) tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGA‑CAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTg < 2:249475/140‑1 (MQ=255) cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCTCAGCAAAAAAAAAAAATGG‑GGTGGGAAGATGAGCGAAAAAAATAAAAAAACAGCAGTGAAAAAAGTTATAGAGAtttt > 2:387958/1‑138 (MQ=255) aggggTAGTTTGTGGCGGTGAATTTTTCCAGA‑CAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACGGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa < 1:43558/137‑1 (MQ=255) aGTGGTAGTTTGTGGCGGTGAATTTTTTCAGA‑CAAAAACAAAAAATGGAGGTGGCCTGATGAGCGGAAATAAAAAACAACAGGCAGGGAAAAAATTGACAGAGAATGTTGCTAAATTTACCGCCATGATTTTTACCCCaa > 2:335419/1‑140 (MQ=255) aGTGGTAGTTTGTGGCGGTGAATTTTTCCAGA‑CAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa < 1:112018/140‑1 (MQ=255) gCGGTGAGTTTTTCAAGA‑AAAATAAAAAAACTGGAGTTGCCATAGTAAGCGAAAATAATAATCAACGGGCAGTAAATAAGTTGACAGGAATTGCCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg < 2:203671/140‑1 (MQ=255) | TGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGA‑CAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTG > NC_000913/3206305‑3206561 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |