| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,140,748 | C→A | A492S (GCC→TCC) | ptsA ← | putative PTS multiphosphoryl transfer protein PtsA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,140,748 | 0 | C | A | 95.7% | 150.3 / 0.7 | 46 | A492S (GCC→TCC) | ptsA | putative PTS multiphosphoryl transfer protein PtsA |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (25/19); total (26/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.27e-01 | |||||||||||
TTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGCGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGAACGGGTTTGCCTCTGCGGGAATGTTCAGATAATCAACGGGTTTGTCGCC > NC_000913/4140617‑4140874 | tttCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAgcggaggagcggagga < 1:292036/140‑1 (MQ=255) tcaAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCg > 1:134044/1‑140 (MQ=255) aCGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGc > 1:532966/1‑140 (MQ=255) tgtgTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTg > 2:582333/1‑140 (MQ=255) tCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTaaa < 1:364589/140‑1 (MQ=255) cGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGCGCGGAGGATCGACCGTAGCTGCg > 1:174165/1‑132 (MQ=255) cGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGCGCGGAGGATCGACCGTAGCTGCg < 2:174165/132‑1 (MQ=255) gTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAacgac > 1:563940/1‑140 (MQ=255) gTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAacgac > 1:490675/1‑140 (MQ=255) gTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGAcgcg < 2:321428/140‑1 (MQ=255) ttGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGt > 2:253840/1‑140 (MQ=255) tGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTa < 2:563940/140‑1 (MQ=255) tGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTa < 1:209307/140‑1 (MQ=255) gCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTAc > 1:449312/1‑140 (MQ=255) tttGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCtt < 2:140232/140‑1 (MQ=255) tttGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCtt > 1:140232/1‑140 (MQ=255) gCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACg < 2:449312/140‑1 (MQ=255) gCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACg > 1:72230/1‑140 (MQ=255) aGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCAc < 1:546659/140‑1 (MQ=255) aGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCAc < 2:134044/140‑1 (MQ=255) aCCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAAcc > 1:78900/1‑140 (MQ=255) aCCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAAcc > 2:47300/1‑140 (MQ=255) tAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGa < 1:253840/140‑1 (MQ=255) tctTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGa > 1:587868/1‑133 (MQ=255) tctTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGa < 2:587868/133‑1 (MQ=255) tctTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGAACGGGtt > 1:388879/1‑140 (MQ=255) tctTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGAACGGGtt > 1:431498/1‑140 (MQ=255) ttCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGAACGGGTTTg > 1:129837/1‑140 (MQ=255) tCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGAACGGGTTTGc < 2:207985/140‑1 (MQ=255) aggagATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGAACGGGTTTGCCTCTGCg > 2:473759/1‑140 (MQ=255) gagaTCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGAACGGGTTTGCTTCTGCggg < 1:582333/140‑1 (MQ=255) gagaTCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGAACGGGTTTGCCTCTGCggg > 1:283963/1‑140 (MQ=255) agaTCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGAACGGGTTTGCCTCTGCGGGa < 2:72230/140‑1 (MQ=255) agaTCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGAACGGGTTTGCCTCTGCGGGa < 2:532966/140‑1 (MQ=255) gaTCATCGGGATCACGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGAACGGGTTTGCCTCTGCGGGaa < 2:163227/140‑1 (MQ=255) catcGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGAACGGGTTTGc > 1:552215/1‑126 (MQ=255) catcGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGAACGGGTTTGc < 2:552215/126‑1 (MQ=255) catcGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGAACGGGTTTGCCTCTGCGGGAATGt > 1:322838/1‑140 (MQ=255) cGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGAACGGGTTTGCCTCTGCGGGa > 2:99370/1‑133 (MQ=255) cGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGAACGGGTTTGCCTCTGCGGGa < 1:99370/133‑1 (MQ=255) cGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGAACGGGTTTGCCTCTGCGGGAATGTTCa > 2:175295/1‑140 (MQ=255) aTTTTCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGAACGGGTTTGCCTCTGCGGGAATGTTCAGATAATCAAc < 2:78900/140‑1 (MQ=255) tCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGAACGGGTTTGCCTCTGCGGGAATGTTCAGATAATCAACGGGt < 2:129837/140‑1 (MQ=255) tCAGGCTGCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGAACGGGTTTGCCTCTGCGGGAATGTTCAGATAATCAACGGGt > 2:73394/1‑140 (MQ=255) gCCGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGAACGGGTTTGCCTCTGCGGGAATGTTCAGATAATCAACGGGTTTGTcgc > 2:412336/1‑140 (MQ=255) ccGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGAACGGGTTTGCCTCTGCGGGAATGTTCAGATAATCAACGGGTTTGTcgcc > 1:274169/1‑140 (MQ=255) ccGTGAGCGGAGGAGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGAACGGGTTTGCCTCTGCGGGAATGTTCAGATAATCAACGGGTTTGTcgcc > 1:413960/1‑140 (MQ=255) | TTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCATGATTTTCAGGCTGCCGTGAGCGGAGGCGCGGAGGATCGACCGTAGCTGCGTGGTAAACAACGACGCGTACTCTTCATAAATACGCACGGCGCGATAACCGAGGAACGGGTTTGCCTCTGCGGGAATGTTCAGATAATCAACGGGTTTGTCGCC > NC_000913/4140617‑4140874 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |