| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,568,243 | C→A | A93S (GCC→TCC) | mdtM ← | multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,568,243 | 0 | C | A | 97.3% | 124.1 / ‑3.6 | 37 | A93S (GCC→TCC) | mdtM | multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (20/16); total (20/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
TTGATCCCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGCGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCGGCCTGCGGCCAATTCTGTCGGAAAGCGGCCCCAGCAGCCACTGTAACGCCATACCGCCAGCAAGATAGAGACTGACGGCAGCAGGGGCCAGA > NC_000913/4568110‑4568376 | ttGATCCCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGc < 1:22970/140‑1 (MQ=255) cTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTa > 2:396824/1‑140 (MQ=255) cTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTa > 2:584535/1‑140 (MQ=255) tcCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAgcg < 1:412887/140‑1 (MQ=255) tcCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACCGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGc < 2:445772/123‑1 (MQ=255) tcCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACCGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGc > 1:445772/1‑123 (MQ=255) aaCGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGcccc > 2:556625/1‑140 (MQ=255) aCGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCg < 1:479571/140‑1 (MQ=255) cGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCgg < 1:396824/140‑1 (MQ=255) ctGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCa > 2:520720/1‑140 (MQ=255) cACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCa > 2:190640/1‑140 (MQ=255) gTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCgg > 1:259176/1‑140 (MQ=255) gTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCgg > 1:215762/1‑140 (MQ=255) cATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGAGCCCCg > 1:338213/1‑122 (MQ=255) cATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGAGCCCCg < 2:338213/122‑1 (MQ=255) aCGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCGGCCt > 1:400105/1‑131 (MQ=255) aCGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCGGCCt < 2:400105/131‑1 (MQ=255) gTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCGGCCTGCGGCCAATTCt > 2:418892/1‑140 (MQ=255) gCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCGGCCTGCGGCCAATTCTGTc > 1:66316/1‑140 (MQ=255) cAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCGGCCTGCGGCCAATTCTGTCg > 1:476093/1‑140 (MQ=255) cAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCGGCCTGCGGCCAATTCTGTCGGAAAGCgg < 1:584535/140‑1 (MQ=255) aTACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCGGCCTGCGGCCAATTCTGTCGGAAAGCGGccc > 2:33999/1‑140 (MQ=255) tGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCGGCCTGCGGCCAATTCTGTCGGAAAGCGGCCCCagcagc < 1:190640/140‑1 (MQ=255) tGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCGGCCTGCGGCCAATTCTGTCGGAAAGCGGCCCCagcagc < 2:522756/140‑1 (MQ=255) aaTTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCGGCCTGCGGCCAATTCTGTCGGAAAGCGGCCCCAGCAGCCACTGTa < 2:215762/140‑1 (MQ=255) gcgATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCGGCCTGCGGCCAATTCTGTCGGAAAGCGGCCCCAGCAGCCACTGTAACGCCATAcc < 1:520720/140‑1 (MQ=255) gATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCGGCCTGCGGCCAATTCTGTCGGAAAGCGGCCCCAGCAGCCACTGTAACGCCATACCGc < 1:289932/140‑1 (MQ=255) tgtCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCGGCCTGCGGCCAATTCTGTCGGAAAGCGGCCCCAGCAGCCACTGTAACGCCATAcc > 2:292920/1‑126 (MQ=255) tgtCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCGGCCTGCGGCCAATTCTGTCGGAAAGCGGCCCCAGCAGCCACTGTAACGCCATAcc < 1:292920/126‑1 (MQ=255) tgtCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCGGCCTGCGGCCAATTCTGTCGGAAAGCGGCCCCAGCAGCCACTGTAACGCCATACCGCCAGCAAGATaga > 2:323751/1‑140 (MQ=255) tCATAGACGTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCGGCCTGCGGCCAATTCTGTCGGAAAGCGGCCCCAGCAGCCACTGTAACGCCATACCGCCAGCAAGATAgaga < 2:66316/140‑1 (MQ=255) gTTGTGAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCGGCCTGCGGCCAATTCTGTCGGAAAGCGGCCCCAGCAGCCACTGTAACGCCATACCGCCAGCAAGATAGAGACTGACGgc > 1:224888/1‑140 (MQ=255) tgtgAACATTGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCGGCCTGCGGCCAATTCTGTCGGAAAGCGGCCCCAGCAGCCACTGTAACGCCATACCGCCAGCAAGATAGAGACTGACGgcag > 2:487994/1‑140 (MQ=255) ttGTCGCGGCGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCGGCCTGCGGCCAATTCTGTCGGAAAGCGGCCCCAGCAGCCACTGTAACGCCATACCGCCAGCAAGATAGAGACTGACGGCAGCAGGGGcc < 1:235402/140‑1 (MQ=255) tGTCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCGGCCTGCGGCCAATTCTGTCGGAAAGCGGCCCCAGCAGCCACTGTAACGCCATACCGCCAGCAAGATAGAGACTGACGGCAGCAGGGGCCa > 2:44331/1‑140 (MQ=255) tCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCGGCCTGCGGCCAATTCTGTCGGAAAGCGGCCCCAGCAGCCACTGTAACGCCATACCGCCAGCAAGATAGAGACTGACGGCAGCAGGGGCCAGa < 1:44331/140‑1 (MQ=255) tCGCGGAGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCGGCCTGCGGCCAATTCTGTCGGAAAGCGGCCCCAGCAGCCACTGTAACGCCATACCGCCAGCAAGATAGAGACTGACGGCAGCAGGGGCCAGa > 2:238521/1‑140 (MQ=255) | TTGATCCCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGCGCAGGCAAGGGTAAAAATTAGCGCCCCGGTAATCAGCACCGGCCTGCGGCCAATTCTGTCGGAAAGCGGCCCCAGCAGCCACTGTAACGCCATACCGCCAGCAAGATAGAGACTGACGGCAGCAGGGGCCAGA > NC_000913/4568110‑4568376 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |