Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	905,528	G→T	G206W (GGG→TGG)	amiD →	N‑acetylmuramoyl‑L‑alanine amidase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	905,528	0	G	T	100.0%	106.3 / NA	31	G206W (GGG→TGG)	amiD	N‑acetylmuramoyl‑L‑alanine amidase D
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/18);  total (13/18)

GGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCC  >  NC_000913/905404‑905663                                                                                                                             |                                                                                                                                        ggCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGcaca                                                                                                                          <  1:298006/140‑1 (MQ=255)    acaTGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCacacac                                                                                                                       <  2:471041/140‑1 (MQ=255)     caTGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACAcc                                                                                                                      >  1:494983/1‑140 (MQ=255)                       cAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCAtt                                                                                                    >  2:522512/1‑140 (MQ=255)                          cgcAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCt                                                                                                 >  2:477393/1‑140 (MQ=255)                                    aTCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTg                                                                                       >  2:343831/1‑140 (MQ=255)                                       ccgggCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGgcg                                                                                    <  2:26334/138‑1 (MQ=255)                                       cGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGgcg                                                                                    <  2:27072/140‑1 (MQ=255)                                         gggCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGgcgcg                                                                                  >  1:564798/1‑140 (MQ=255)                                          ggCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGgcgcgc                                                                                 <  2:448274/140‑1 (MQ=255)                                           gCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGgcg                                                                                    >  2:58093/1‑136 (MQ=255)                                           gCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGgcg                                                                                    <  1:58093/136‑1 (MQ=255)                                                   ttCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTAt                                                                        <  1:522512/140‑1 (MQ=255)                                                       cTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACg                                                                    <  1:477393/140‑1 (MQ=255)                                                        tGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGt                                                                   <  2:494983/140‑1 (MQ=255)                                                         ggCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGtt                                                                  >  2:346532/1‑140 (MQ=255)                                                         ggCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGtt                                                                  >  2:298123/1‑140 (MQ=255)                                                              caACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAAcc                                                             <  1:298123/140‑1 (MQ=255)                                                              caACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAAcc                                                             <  2:220940/140‑1 (MQ=255)                                                              caACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAAcc                                                             <  1:346783/140‑1 (MQ=255)                                                              caACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAAcc                                                             <  1:346532/140‑1 (MQ=255)                                                              caACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAAcc                                                             <  1:343831/140‑1 (MQ=255)                                                                        agcagGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGaca                                                   >  1:540065/1‑140 (MQ=255)                                                                                  tGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGagc                                         <  1:362974/140‑1 (MQ=255)                                                                                    gTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGagcag                                       >  1:596982/1‑140 (MQ=255)                                                                                          ggCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGagca                                        >  2:434563/1‑133 (MQ=255)                                                                                          ggCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGagca                                        <  1:434563/133‑1 (MQ=255)                                                                                                             tAACTTTTACCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGa              >  2:216906/1‑140 (MQ=255)                                                                                                                     aCCTTGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTc      >  1:385734/1‑140 (MQ=255)                                                                                                                         tGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGcc  <  2:540065/140‑1 (MQ=255)                                                                                                                         tGCCTGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGcc  <  2:564798/140‑1 (MQ=255)                                                                                                                             |                                                                                                                                        GGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCC  >  NC_000913/905404‑905663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑