Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,630,593	G→T	E90* (GAA→TAA)	yfgI →	nalidixic acid resistance protein YfgI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,630,593	0	G	T	100.0%	140.5 / NA	40	E90* (GAA→TAA)	yfgI	nalidixic acid resistance protein YfgI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (25/15);  total (25/15)

CGAATGGGTCGCGGAGCGCAACAGGGAAATCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCGAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACGAATTTATCGATCAGGAAC  >  NC_000913/2630460‑2630728                                                                                                                                      |                                                                                                                                        cGAATGGGTCGCGGAGCGCAACAGGGAAATCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCct                                                                                                                                   >  1:84688/1‑140 (MQ=255)          cgcgGAGCGCAACAGGGAAATCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACa                                                                                                                          >  2:184166/1‑140 (MQ=255)           gcgGAGCGCAACAGGGAAATCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACaa                                                                                                                         >  1:231267/1‑140 (MQ=255)           gcgGAGCACAACAGGGAAATCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACaa                                                                                                                         >  1:511878/1‑140 (MQ=255)            cgGAGCGCAACAGGGAAATCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACaaa                                                                                                                        >  2:535349/1‑140 (MQ=255)            cgGAGCGCAACAGGGAAATCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACaaa                                                                                                                        >  1:93948/1‑140 (MQ=255)                     aCAGGGAAATCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGAt                                                                                                               <  2:301782/140‑1 (MQ=255)                     aCAGGGAAATCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGAt                                                                                                               <  2:231267/140‑1 (MQ=255)                      cAGGGAAATCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATc                                                                                                              >  2:431507/1‑140 (MQ=255)                       aGGGAAATCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCa                                                                                                             <  2:84688/140‑1 (MQ=255)                             aTCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTa                                                                                                       <  2:93948/140‑1 (MQ=255)                                  gCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAg                                                                                                  <  2:408273/140‑1 (MQ=255)                                   cAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGa                                                                                                 >  2:477732/1‑140 (MQ=255)                                           gCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTAc                                                                                         <  2:478188/140‑1 (MQ=255)                                            cAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACg                                                                                        <  2:511878/140‑1 (MQ=255)                                            cAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACg                                                                                        <  1:431507/140‑1 (MQ=255)                                            cAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACg                                                                                        <  1:184166/140‑1 (MQ=255)                                                         aTGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACg                                                                                        >  2:271588/1‑127 (MQ=255)                                                         aTGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACg                                                                                        <  1:271588/127‑1 (MQ=255)                                                                     ccgcTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACaaaaa                                                               >  1:567573/1‑140 (MQ=255)                                                                      cgcTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGTCGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAAc                                                              >  2:405437/1‑140 (MQ=255)                                                                      cgcTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAAc                                                              >  1:77511/1‑140 (MQ=255)                                                                      cgcTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAAc                                                              >  2:197318/1‑140 (MQ=255)                                                                      cgcTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAAc                                                              >  1:240779/1‑140 (MQ=255)                                                                           aCAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAActggcg                                                         <  1:477732/140‑1 (MQ=255)                                                                           aCAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAActggcg                                                         <  1:138072/140‑1 (MQ=255)                                                                                             cAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGcc                                       <  1:197318/140‑1 (MQ=255)                                                                                                     aaTAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTaa                               >  1:105189/1‑140 (MQ=255)                                                                                                             aGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGaaa                       >  1:400072/1‑140 (MQ=255)                                                                                                               cTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAAc                     >  1:126417/1‑140 (MQ=255)                                                                                                                tCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACg                    >  1:420307/1‑140 (MQ=255)                                                                                                                 cGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACGa                   >  1:371247/1‑140 (MQ=255)                                                                                                                  gTCAGGTTAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGATAACGaa                  >  1:16462/1‑140 (MQ=255)                                                                                                                  gTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACGaa                  >  2:64506/1‑140 (MQ=255)                                                                                                                    cAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACGAAtt                <  1:535349/140‑1 (MQ=255)                                                                                                                       gATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACGAATTTAt             <  2:77511/140‑1 (MQ=255)                                                                                                                         tAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACGAATTTATCg           >  1:426886/1‑140 (MQ=255)                                                                                                                            aCTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACGAATTTATCGATc        >  1:428587/1‑140 (MQ=255)                                                                                                                                 aCGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACGAATTTATCGATCAGGaa   >  2:82305/1‑140 (MQ=255)                                                                                                                                  cGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACGAATTTATCGATCAGGAAc  >  2:339351/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        CGAATGGGTCGCGGAGCGCAACAGGGAAATCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCGAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACGAATTTATCGATCAGGAAC  >  NC_000913/2630460‑2630728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑