Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,036,345	C→A	intergenic (‑63/+28)	prfB ← / ← recJ	peptide chain release factor RF2/ssDNA‑specific exonuclease RecJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,036,345	0	C	A	100.0%	152.6 / NA	44	intergenic (‑63/+28)	prfB/recJ	peptide chain release factor RF2/ssDNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/22);  total (22/22)

AGATACCCCCTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTAT  >  NC_000913/3036209‑3036476                                                                                                                                         |                                                                                                                                    aGATACCCCCTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACg                                                                                                                                  <  2:242271/140‑1 (MQ=255) aGATACCCCCTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACg                                                                                                                                  <  1:297990/140‑1 (MQ=255)   aTACCCCCTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGct                                                                                                                                <  2:205045/140‑1 (MQ=255)   aTACCCCCTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGct                                                                                                                                <  1:74215/140‑1 (MQ=255)   aTACCCCCTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGct                                                                                                                                <  1:646195/140‑1 (MQ=255)   aTACCCCCTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGct                                                                                                                                <  1:578907/140‑1 (MQ=255)      cccccTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCtt                                                                                                                             >  2:586436/1‑140 (MQ=255)       ccccTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCttt                                                                                                                            >  1:170914/1‑140 (MQ=255)       ccccTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCttt                                                                                                                            >  2:501757/1‑140 (MQ=255)         ccTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCtttt                                                                                                                           <  1:228339/139‑1 (MQ=255)         ccTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCtttt                                                                                                                           >  2:228339/1‑139 (MQ=255)         ccTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCtt                                                                                                                             <  1:317009/137‑1 (MQ=255)         ccTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCtt                                                                                                                             >  2:317009/1‑137 (MQ=255)                   tCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGagaag                                                                                                                >  2:451714/1‑140 (MQ=255)                         cGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACg                                                                                                          <  1:533164/140‑1 (MQ=255)                                      ccTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAg                                                                                             >  1:375326/1‑140 (MQ=255)                                       cTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGa                                                                                            >  1:78178/1‑140 (MQ=255)                                        tGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGat                                                                                           >  1:251902/1‑140 (MQ=255)                                               gATTATTTACCGGATTAATTTCAACCATGGTCTGATTTCTTTTATTGAGCTAGTC‑AAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCga                                                                                   <  2:443349/140‑1 (MQ=255)                                                attattTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCga                                                                                   <  1:586436/140‑1 (MQ=255)                                                attattTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCga                                                                                   <  2:375326/140‑1 (MQ=255)                                                attattTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCga                                                                                   <  2:78178/140‑1 (MQ=255)                                                  tattTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCgatg                                                                                 >  2:43161/1‑140 (MQ=255)                                                           gATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGata                                                                                          <  1:193029/122‑1 (MQ=255)                                                           gATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGata                                                                                          >  2:193029/1‑122 (MQ=255)                                                           gATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGc                                                                        >  2:650558/1‑140 (MQ=255)                                                              tAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAgg                                                                     >  1:44483/1‑140 (MQ=255)                                                                               tGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGaaa                                                    <  2:44483/140‑1 (MQ=255)                                                                                       tttATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGa                                            >  2:601855/1‑140 (MQ=255)                                                                                                   gTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTtat                                >  1:96069/1‑140 (MQ=255)                                                                                                          tGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAg                         >  2:14608/1‑140 (MQ=255)                                                                                                             ggTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATaa                              <  2:3779/132‑1 (MQ=255)                                                                                                             ggTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATaa                              >  1:3779/1‑132 (MQ=255)                                                                                                                       gCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGGTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCgcgc            <  1:601855/140‑1 (MQ=255)                                                                                                                        cGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCa           <  2:170914/140‑1 (MQ=255)                                                                                                                         gggATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCAc          >  1:311448/1‑140 (MQ=255)                                                                                                                          ggATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACg         >  2:365853/1‑140 (MQ=255)                                                                                                                            aTTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGcc       <  1:451714/140‑1 (MQ=255)                                                                                                                                tACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCgcgc            >  1:21182/1‑131 (MQ=255)                                                                                                                                tACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCgcgc            <  2:21182/131‑1 (MQ=255)                                                                                                                                tACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTa   >  2:173377/1‑140 (MQ=255)                                                                                                                                 aCCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTAt  <  2:59231/140‑1 (MQ=255)                                                                                                                                     aaTCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCa           >  1:632079/1‑127 (MQ=255)                                                                                                                                     aaTCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCa           <  2:632079/127‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    AGATACCCCCTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTAT  >  NC_000913/3036209‑3036476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑