Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,172,601	G→T	L24L (CTG→CTT)	mdaB →	NADPH:quinone oxidoreductase MdaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,172,601	0	G	T	100.0%	188.4 / NA	55	L24L (CTG→CTT)	mdaB	NADPH:quinone oxidoreductase MdaB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (31/24);  total (31/24)

TCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTGACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAACTTTCTCTGGGCTGATGTGGTGATCTGGCAGATGCCAGGC  >  NC_000913/3172471‑3172736                                                                                                                                   |                                                                                                                                        tCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTc                                                                                                                                <  1:420336/140‑1 (MQ=255)   aCCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTcgc                                                                                                                              <  1:488572/140‑1 (MQ=255)   aCCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTcgc                                                                                                                              >  1:270376/1‑140 (MQ=255)     cGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCgg                                                                                                                            <  2:681074/140‑1 (MQ=255)                 tgACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTcgc                                                                                                                              >  1:530495/1‑126 (MQ=255)                 tgACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTcgc                                                                                                                              <  2:530495/126‑1 (MQ=255)                 tgACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACAGAAGTCGCGGATGGCACACTgc                                                                                                                >  2:326021/1‑140 (MQ=255)                      tACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGcaca                                                                                                                    <  2:57106/131‑1 (MQ=255)                      tACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGcaca                                                                                                                    >  1:57106/1‑131 (MQ=255)                      tACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGAc                                                                                                           >  1:156441/1‑140 (MQ=255)                        cTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGTCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCt                                                                                                         <  1:547112/140‑1 (MQ=255)                               cGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCat                                                                                                  <  2:700654/140‑1 (MQ=255)                                ggCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCTCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCatg                                                                                                 >  1:427581/1‑140 (MQ=255)                                ggCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCTAcc                                                                                                          <  2:240374/131‑1 (MQ=255)                                ggCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCTAcc                                                                                                          >  1:240374/1‑131 (MQ=255)                                ggCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCatg                                                                                                 >  2:518158/1‑140 (MQ=255)                                ggCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCatg                                                                                                 >  2:253364/1‑140 (MQ=255)                                ggCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCatg                                                                                                 >  1:436613/1‑140 (MQ=255)                                  cAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCatgat                                                                                               >  2:693428/1‑140 (MQ=255)                                  cAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCatgat                                                                                               >  1:422891/1‑140 (MQ=255)                                              cTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGtt                                                                                   <  1:298666/140‑1 (MQ=255)                                                 gAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTcgc                                                                                >  2:517647/1‑140 (MQ=255)                                                  aGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTcgcg                                                                               <  1:253364/140‑1 (MQ=255)                                                      tAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACAGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGa                                                                           <  1:326021/140‑1 (MQ=255)                                                              gAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACt                                                                   >  2:433276/1‑140 (MQ=255)                                                               aGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTa                                                                  >  2:7702/1‑140 (MQ=255)                                                                       ccTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCa                                                          >  1:579059/1‑140 (MQ=255)                                                                          gATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAg                                                       <  2:642800/140‑1 (MQ=255)                                                                             tATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCgg                                                    <  1:371153/140‑1 (MQ=255)                                                                              aTCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGa                                                   >  2:584756/1‑140 (MQ=255)                                                                                cAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAg                                                 >  2:368449/1‑140 (MQ=255)                                                                                   cGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTAc                                              <  1:584756/140‑1 (MQ=255)                                                                                      cgcgAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACaaa                                           >  2:416504/1‑140 (MQ=255)                                                                                       gcgAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACaaaa                                          >  2:361819/1‑140 (MQ=255)                                                                                                  cGCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAACTTTCTCTggg                               <  2:270376/140‑1 (MQ=255)                                                                                                   gCCCGCTCCAATGGTCAACTGAACGCCGCCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAGCTTTCTCTGGGc                              <  1:426583/140‑1 (MQ=255)                                                                                                   gCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAACTTTCTCTGGGc                              <  1:518158/140‑1 (MQ=255)                                                                                                   gCCCACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAACTTTCTCTGGGc                              <  1:368449/140‑1 (MQ=255)                                                                                                     ccACTCCAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAACTTTCTCTGGGCTg                            <  2:422891/140‑1 (MQ=255)                                                                                                           cAATGGTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAACTTTCTCTGGGCTGATgtgg                      <  2:156441/140‑1 (MQ=255)                                                                                                               ggTCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAACTTTCTCTGGGCTGATGTGGTGAt                  <  2:354815/140‑1 (MQ=255)                                                                                                                 tCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAACTTTCTCTGGGCTGAtg                         >  2:619114/1‑131 (MQ=255)                                                                                                                 tCAACTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAACTTTCTCTGGGCTGAtg                         <  1:619114/131‑1 (MQ=255)                                                                                                                   aaCTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAACTTTCTCTGGGCTGAtgt                        >  2:443268/1‑130 (MQ=255)                                                                                                                   aaCTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAACTTTCTCTGGGCTGAtgt                        <  1:443268/130‑1 (MQ=255)                                                                                                                   aaCTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAACTTTCTCTGGGCTGATGTGGTGATCTgg              >  2:40186/1‑140 (MQ=255)                                                                                                                   aaCTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAACTTTCTCTGGGCTGATGTGGTGATCTgg              >  2:551420/1‑140 (MQ=255)                                                                                                                   aaCTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAACTTTCTCTGGGCTGATGTGGTGATCTgg              >  2:140921/1‑140 (MQ=255)                                                                                                                   aaCTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAACTTTCTCTGGGCTGATGTGGTGATCTgg              >  1:602580/1‑140 (MQ=255)                                                                                                                   aaCTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAACTTTCTCTGGGCTGATGTGGTGATCTgg              >  1:513529/1‑140 (MQ=255)                                                                                                                    aCTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAACTTTCTCTGGGCTGATGTGGTGATCTGGc             <  2:427581/140‑1 (MQ=255)                                                                                                                    aCTGAACGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAACTTTCTCTGGGCTGATGTGGTGACCTGGc             <  2:552382/140‑1 (MQ=255)                                                                                                                        aaCGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAACTTTCTCTGGGCTGATGTGGTGATCTGGCAGAt         >  1:353927/1‑140 (MQ=255)                                                                                                                        aaCGACACCCTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAACTTTCTCTGGGCTGATGTGGTGATCTGGCAGAt         >  2:345433/1‑140 (MQ=255)                                                                                                                               cccTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAACTTTCTCTGGGCTGATGTGGTGATCTGGCAGATGCCAGGc  <  2:436613/140‑1 (MQ=255)                                                                                                                               cccTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAACTTTCTCTGGGCTGATGTGGTGATCTGGCAGATGCCAGGc  >  2:387710/1‑140 (MQ=255)                                                                                                                               cccTTACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAACTTTCTCTGGGCTGATGTGGTGATCTGGCAGATGCCAGGc  >  2:284270/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                        TCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTGACCGAAGTCGCGGATGGCACACTGCGCGACCTTGGGCATGATGTCCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAGTACAAAACTTTCTCTGGGCTGATGTGGTGATCTGGCAGATGCCAGGC  >  NC_000913/3172471‑3172736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑