Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,354,487	C→T	intergenic (‑437/‑238)	yhcC ← / → gltB	radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,354,487	0	C	T	100.0%	110.6 / NA	32	intergenic (‑437/‑238)	yhcC/gltB	radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/16);  total (16/16)

TCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGG  >  NC_000913/3354352‑3354610                                                                                                                                        |                                                                                                                            tCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCt                                                                                                                         >  2:156749/1‑140 (MQ=255)           aCTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATaa                                                                                                               >  1:341609/1‑140 (MQ=255)                         gACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCt                                                                                                 >  2:149534/1‑140 (MQ=255)                         gACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCt                                                                                                 >  2:600268/1‑140 (MQ=255)                         gACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCt                                                                                                 >  1:286898/1‑140 (MQ=255)                            ttCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGa                                                                                              >  2:394580/1‑140 (MQ=255)                             acccTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGaa                                                                                             >  2:521513/2‑140 (MQ=255)                              cccTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAg                                                                                            <  2:341609/140‑1 (MQ=255)                              cccTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAg                                                                                            <  1:698293/140‑1 (MQ=255)                                 ttttAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCtt                                                                                         <  1:156749/140‑1 (MQ=255)                                      gCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTgg                                                                                    >  2:381325/1‑140 (MQ=255)                                        cTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGAt                                                                                  <  2:6267/140‑1 (MQ=255)                                          tAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGa                                                                                <  1:381325/140‑1 (MQ=255)                                           aaaGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGag                                                                               >  1:225815/1‑140 (MQ=255)                                                  aaaTCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTg                                                                        <  1:521513/140‑1 (MQ=255)                                                  aaaTCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTg                                                                        <  1:600268/140‑1 (MQ=255)                                                          tttAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTcatcat                                                                <  1:677478/140‑1 (MQ=255)                                                                ttCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTAt                                                          <  1:206638/140‑1 (MQ=255)                                                                 tCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATg                                                         <  1:206096/140‑1 (MQ=255)                                                                 tCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATg                                                         <  2:214780/140‑1 (MQ=255)                                                                    gTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTa                                                           >  1:328301/1‑135 (MQ=255)                                                                    gTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTa                                                           <  2:328301/135‑1 (MQ=255)                                                                    gTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAg                                                      <  1:201305/140‑1 (MQ=255)                                                                         ttAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAAtt                                                 <  1:149534/140‑1 (MQ=255)                                                                                                        cTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCa                                  <  1:144084/124‑1 (MQ=255)                                                                                                        cTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCa                                  >  2:144084/1‑124 (MQ=255)                                                                                                           tACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTTGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGaa               >  2:95789/1‑140 (MQ=255)                                                                                                                aTAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGa          >  1:707442/1‑140 (MQ=255)                                                                                                                aTAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGa          >  1:48423/1‑140 (MQ=255)                                                                                                                aTAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGa          >  1:12148/1‑140 (MQ=255)                                                                                                                aTAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGa          >  2:682276/1‑140 (MQ=255)                                                                                                                 tAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGac         <  2:225815/140‑1 (MQ=255)                                                                                                                        gCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAgg  >  1:221957/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                            TCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGG  >  NC_000913/3354352‑3354610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑