Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	391,716	A→C	intergenic (+8/+23)	yaiT → / ← insF‑2	putative autotransporter YaiT/IS3 element protein InsF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	391,716	0	A	C	100.0%	234.0 / NA	65	intergenic (+8/+23)	yaiT/insF‑2	putative autotransporter YaiT/IS3 element protein InsF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (34/31);  total (34/31)

AGATACTATCGATTTAACTAACGGTTCTTCGCTGAACATTGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCG  >  NC_000913/391579‑391846                                                                                                                                          |                                                                                                                                   aGATACTATCGATTTAACTAACGGTTCTTCGCTGAACATTGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTccc                                                                                                                                  >  2:145190/1‑140 (MQ=255)    tACTATCGATTTAACTAACGGTTCTTCGCTGAACATTGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCAc                                                                                                                               <  2:221164/140‑1 (MQ=255)      cTATCGATTTAACTAACGGTTCTTCGCTGAACATTGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGt                                                                                                                             >  1:232983/1‑140 (MQ=255)      cTATCGATTTAACTAACGGTTCTTCGCTGAACATTGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGt                                                                                                                             <  2:136193/140‑1 (MQ=255)       tATCGATTTAACTAACGGTTCTTCGCTGAACATTGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTa                                                                                                                            >  1:629129/1‑140 (MQ=255)        aTCGATTTAACTAACGGTTCTTCGCTGAACATTGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTaa                                                                                                                           >  1:591271/1‑140 (MQ=255)        aTCGATTTAACTAACGGTTCTTCGCTGAACATTGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTaa                                                                                                                           >  2:342435/1‑140 (MQ=255)        aTCGATTTAACTAACGGTTCTTCGCTGAACATTGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTaa                                                                                                                           >  2:338458/1‑140 (MQ=255)           gATTTAACTAACGGTTCTTCGCTGAACATTGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAatat                                                                                                                        <  1:30226/140‑1 (MQ=255)                 actaacGGTTCTTCGCTGAACATTGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGacac                                                                                                                  <  1:145190/140‑1 (MQ=255)                 actaacGGTTCTTCGCTGAACATTGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGacac                                                                                                                  >  2:395957/1‑140 (MQ=255)                     acGGTTCTTCGCTGAACATTGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGc                                                                                                              >  2:248088/1‑140 (MQ=255)                      cGGTTCTTCGCTGAACATTGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGcc                                                                                                             >  2:581816/1‑140 (MQ=255)                           cttcGCTGAACATTGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGc                                                                                                        >  1:661364/1‑140 (MQ=255)                               gCTGAACATTGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAgg                                                                                                    <  1:395957/140‑1 (MQ=255)                                   aaCATTGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCt                                                                                                >  2:124867/1‑140 (MQ=255)                                             aaGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCaaa                                                                                      <  1:248088/140‑1 (MQ=255)                                             aaGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCaaa                                                                                      <  2:386796/140‑1 (MQ=255)                                               gATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAAtt                                                                                    >  1:16669/1‑140 (MQ=255)                                                   gCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTc                                                                                <  2:278665/140‑1 (MQ=255)                                                      aCGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCgg                                                                             <  1:540974/140‑1 (MQ=255)                                                            aTACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCCCGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAg                                                                       >  1:103520/1‑140 (MQ=255)                                                               ccGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCCCGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGgcc                                                                    <  2:103520/140‑1 (MQ=255)                                                                 gATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGgccgc                                                                  <  2:629129/140‑1 (MQ=255)                                                                 gATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGgccgc                                                                  <  2:661364/140‑1 (MQ=255)                                                                  atcatcTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGgccgcc                                                                 >  1:482518/1‑140 (MQ=255)                                                                  atcatcTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGgccgcc                                                                 >  2:149076/1‑140 (MQ=255)                                                                  atcatcTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGgccgcc                                                                 >  1:267060/1‑140 (MQ=255)                                                                  atcatcTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGgccgcc                                                                 >  1:651242/1‑140 (MQ=255)                                                                      tcTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGgccgc                                                                  >  1:349746/1‑135 (MQ=255)                                                                      tcTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGgccgc                                                                  <  2:349746/135‑1 (MQ=255)                                                                      tcTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCacac                                                             >  2:235559/1‑140 (MQ=255)                                                                       cTGACTATTAACTCCTACAGTACTGTTGCTTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACAcc                                                            <  2:71414/140‑1 (MQ=255)                                                                       cTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACAcc                                                            >  1:568869/1‑140 (MQ=255)                                                                           cTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACt                                                        >  2:253289/1‑140 (MQ=255)                                                                               tAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTgc                                                    <  2:568869/140‑1 (MQ=255)                                                                                    ccTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTgccgccg                                               >  1:408623/1‑140 (MQ=255)                                                                                     cTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTaccgccgc                                              >  1:133448/1‑140 (MQ=255)                                                                                        cAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCAc                                           >  1:26495/1‑140 (MQ=255)                                                                                         aGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCAcc                                          <  2:482518/140‑1 (MQ=255)                                                                                           tACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCg                                         >  2:157264/1‑139 (MQ=255)                                                                                           tACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCg                                         <  1:157264/139‑1 (MQ=255)                                                                                           tACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGa                                        >  1:429632/1‑140 (MQ=255)                                                                                           tACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGa                                        <  1:149076/140‑1 (MQ=255)                                                                                            aCTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGAt                                       >  2:237295/1‑140 (MQ=255)                                                                                             cTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGAtt                                      >  2:306711/1‑140 (MQ=255)                                                                                             cTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCACACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGAtt                                      >  1:496621/1‑140 (MQ=255)                                                                                                ttGCGTTGACCGAATCTACTGGGGGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTa                                   >  2:93406/1‑140 (MQ=255)                                                                                                  gCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAAt                                 <  1:306121/140‑1 (MQ=255)                                                                                                     ttGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATcac                              >  2:699051/1‑140 (MQ=255)                                                                                                      tGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATcaca                             <  2:267060/140‑1 (MQ=255)                                                                                                      tGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATcaca                             <  1:699051/140‑1 (MQ=255)                                                                                                      tGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATcaca                             <  2:616771/140‑1 (MQ=255)                                                                                                        aCCGAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACAtt                           <  1:338458/140‑1 (MQ=255)                                                                                                           gAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGa                        <  2:408623/140‑1 (MQ=255)                                                                                                           gAATCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGa                        <  2:591271/140‑1 (MQ=255)                                                                                                            aaTCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTACCGCCGCCACCGATTGTAATCACATTCGat                       <  2:133448/140‑1 (MQ=255)                                                                                                             aTCTACTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGata                      <  2:16669/140‑1 (MQ=255)                                                                                                                  cTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATcaca                             <  2:158598/128‑1 (MQ=255)                                                                                                                  cTGGGTGGGGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATcaca                             >  1:158598/1‑128 (MQ=255)                                                                                                                      gtgggGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGata                      <  2:184446/131‑1 (MQ=38)                                                                                                                      gtgggGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGata                      >  1:184446/1‑131 (MQ=38)                                                                                                                       tgggGGGCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAaca            >  1:18184/1‑140 (MQ=37)                                                                                                                            ggCTGATTGATCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGtt       <  1:124867/140‑1 (MQ=18)                                                                                                                               tgattgatCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGcc    >  1:205046/1‑140 (MQ=17)                                                                                                                                gattgatCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGccc   <  2:101550/140‑1 (MQ=14)                                                                                                                                 attgatCCTCCCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCg  >  1:251578/1‑140 (MQ=11)                                                                                                                                          |                                                                                                                                   AGATACTATCGATTTAACTAACGGTTCTTCGCTGAACATTGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCG  >  NC_000913/391579‑391846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑