Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	747,140	C→A	P210P (CCG→CCT)	abrB ←	putative regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	747,140	0	C	A	96.4%	89.6 / ‑2.8	28	P210P (CCG→CCT)	abrB	putative regulator
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (18/9);  total (19/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01

TCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATCGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAA  >  NC_000913/747043‑747264                                                                                                  |                                                                                                                             tCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATccc                                                                                    >  2:694095/1‑140 (MQ=255)       agccaATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTa                                                                              >  2:676203/1‑140 (MQ=255)                ccATATACACCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACg                                                                     >  1:112281/1‑140 (MQ=255)                   tataCGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGAcc                                                                  >  1:522084/1‑140 (MQ=255)                     taCGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACcgc                                                                >  1:560949/1‑140 (MQ=255)                        gCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAg                                                             >  1:8888/1‑140 (MQ=255)                          cATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATCGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCa                                                           >  2:568497/1‑140 (MQ=255)                          cATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCa                                                           >  1:476759/1‑140 (MQ=255)                          cATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCa                                                           >  2:22517/1‑140 (MQ=255)                          cATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCa                                                           >  2:26739/1‑140 (MQ=255)                          cATCGCCAGCAGCCATTCCGGTAGTTCGAT‑GTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCaa                                                          >  1:337103/1‑140 (MQ=255)                             cGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCaaaa                                                        >  1:545922/1‑140 (MQ=255)                                      ccATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCa                                               >  2:615099/1‑140 (MQ=255)                                         ttCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGga                                            <  1:22517/140‑1 (MQ=255)                                              gTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTa                                       >  1:295593/1‑140 (MQ=255)                                                aGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAAt                                     >  1:374947/1‑140 (MQ=255)                                                 gTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATg                                    <  1:26739/140‑1 (MQ=255)                                                     gATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCa                                <  2:522084/140‑1 (MQ=255)                                                      aTGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATg                                    >  1:480721/1‑135 (MQ=255)                                                      aTGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATg                                    <  2:480721/135‑1 (MQ=255)                                                         gTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTgg                            >  2:393579/1‑140 (MQ=255)                                                             tgaGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCggg                        <  2:295593/140‑1 (MQ=255)                                                                gCTGACCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAAc                     <  2:337103/140‑1 (MQ=255)                                                                 cTGACCGGGCTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAAcc                    <  1:694095/140‑1 (MQ=255)                                                                    aCCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTgg                            <  2:416289/129‑1 (MQ=255)                                                                    aCCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTgg                            >  1:416289/1‑129 (MQ=255)                                                                    aCCAGACTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGa                 <  2:476759/140‑1 (MQ=255)                                                                         aCTGGAGCACTGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGata            >  1:442806/1‑140 (MQ=255)                                                                                   tGCGCCCGCCAGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTaa  >  2:557152/1‑140 (MQ=255)                                                                                                  |                                                                                                                             TCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATCGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAA  >  NC_000913/747043‑747264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑