Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,255,245	G→T	intergenic (‑59/‑110)	rihB ← / → yeiL	pyrimidine‑specific ribonucleoside hydrolase RihB/putative DNA‑binding transcriptional regulator YeiL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,255,245	0	G	T	100.0%	162.6 / NA	47	intergenic (‑59/‑110)	rihB/yeiL	pyrimidine‑specific ribonucleoside hydrolase RihB/putative DNA‑binding transcriptional regulator YeiL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/30);  total (17/30)

GATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTAAGCAGCAATTGATGAGTGAATCCGCGTTT  >  NC_000913/2255108‑2255372                                                                                                                                          |                                                                                                                                gATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCtgg                                                                                                                               >  2:95948/1‑140 (MQ=255)      ttCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCgat                                                                                                                          >  2:476530/1‑140 (MQ=255)            gccATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGa                                                                                                                    <  2:191026/140‑1 (MQ=255)            gccATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGa                                                                                                                    <  1:698567/140‑1 (MQ=255)            gccATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGa                                                                                                                    <  1:643337/140‑1 (MQ=255)                 catTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCtt                                                                                                               <  1:643154/140‑1 (MQ=255)                      tagcaatagcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTaacaa                                                                                                          <  2:36842/140‑1 (MQ=255)                      tagcaatagcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTaacaa                                                                                                          <  1:673324/140‑1 (MQ=255)                           atagcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGcg                                                                                                     >  2:98462/1‑140 (MQ=255)                            tagcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGcgt                                                                                                    >  2:431045/1‑140 (MQ=255)                            tagcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGcgt                                                                                                    >  2:596315/1‑140 (MQ=255)                                   atcatGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATg                                                                                             >  1:542802/1‑140 (MQ=255)                                    tcatGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGcgtacg                                                                                                 <  1:451743/135‑1 (MQ=255)                                    tcatGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGcgtacg                                                                                                 >  2:451743/1‑135 (MQ=255)                                      atGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTa                                                                                          <  1:476530/140‑1 (MQ=255)                                              gATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATgag                                                                                  >  1:38654/1‑140 (MQ=255)                                              gATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATgag                                                                                  <  2:480992/140‑1 (MQ=255)                                              gATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATgag                                                                                  <  2:542802/140‑1 (MQ=255)                                                   caATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAAtc                                                                             >  1:536969/1‑140 (MQ=255)                                                         aGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAg                                                                       >  1:376461/1‑140 (MQ=255)                                                           aataatTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCg                                                                     >  1:318159/1‑140 (MQ=255)                                                           aataatTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCg                                                                     >  2:217900/1‑140 (MQ=255)                                                                tttttCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATaa                                                                >  1:29872/1‑140 (MQ=255)                                                                              aTTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTgaaagaa                                                  <  2:470803/140‑1 (MQ=255)                                                                              aTTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTgaaagaa                                                  <  1:27696/140‑1 (MQ=255)                                                                               ttGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTgaaagaaa                                                 >  2:661645/1‑140 (MQ=255)                                                                                 gTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGCTAAACATCGTGAAAGAAATc                                               <  2:376461/140‑1 (MQ=255)                                                                                  tttATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATcc                                              >  1:329141/1‑140 (MQ=255)                                                                                   ttATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCca                                             <  1:660887/140‑1 (MQ=255)                                                                                   ttATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCAACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCca                                             <  1:596315/140‑1 (MQ=255)                                                                                         ccTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACaataa                                       <  1:95948/140‑1 (MQ=255)                                                                                         ccTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACaataa                                       <  2:329141/140‑1 (MQ=255)                                                                                         ccTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACaataa                                       <  1:187439/140‑1 (MQ=255)                                                                                         ccTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACaataa                                       <  1:431131/140‑1 (MQ=255)                                                                                            ctGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACaata                                        >  1:435705/1‑136 (MQ=255)                                                                                            ctGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACaata                                        <  2:435705/136‑1 (MQ=255)                                                                                                 tCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTa                               <  2:135953/140‑1 (MQ=255)                                                                                                 tCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTa                               >  2:233255/1‑140 (MQ=255)                                                                                                    aGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTAagc                            <  1:661645/140‑1 (MQ=255)                                                                                                    aGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTAagc                            <  1:233255/140‑1 (MQ=255)                                                                                                            tATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTAAGCAGCAATtg                    <  2:38654/140‑1 (MQ=255)                                                                                                            tATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTAAGCAGCAATtg                    <  2:29872/140‑1 (MQ=255)                                                                                                                   tCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTAAGCAGCAATtg                    >  2:415437/1‑133 (MQ=255)                                                                                                                   tCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTAAGCAGCAATtg                    <  1:415437/133‑1 (MQ=255)                                                                                                                      aCAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTAAGCAGCAATTGATGAGTGAAt          <  2:318159/140‑1 (MQ=255)                                                                                                                       cAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTAAGCAGCAATTGATGAGTGAATc         <  2:536969/140‑1 (MQ=255)                                                                                                                        aGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTAAGCAGCAATTGATGAGTGAATcc        <  2:250797/140‑1 (MQ=255)                                                                                                                              gAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTAAGCAGCAATTGATGAGTGAATCCGCGttt  <  1:431045/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                GATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTAAGCAGCAATTGATGAGTGAATCCGCGTTT  >  NC_000913/2255108‑2255372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑