Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	106,759	A→G	N68S (AAC→AGC)	lpxC →	UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	106,759	0	A	G	100.0%	155.7 / NA	42	N68S (AAC→AGC)	lpxC	UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/26);  total (16/26)

AGTCACCCTGACGTTACGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATA  >  NC_000913/106625‑106894                                                                                                                                       |                                                                                                                                        aGTCACCCTGACGTTACGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGc                                                                                                                                    <  1:386053/140‑1 (MQ=255)  gTCACCCTGACGTTACGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCa                                                                                                                                   <  1:647541/140‑1 (MQ=255)  gTCACCCTGACGTTACGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCa                                                                                                                                   <  1:479628/140‑1 (MQ=255)              ttACGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGAt                                                                                                                       <  1:337628/140‑1 (MQ=255)                aCGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGAttt                                                                                                                     <  1:721635/140‑1 (MQ=255)                 cGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTc                                                                                                                    <  2:785906/140‑1 (MQ=255)                      tGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCg                                                                                                               <  2:788361/140‑1 (MQ=255)                       gcgcCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGt                                                                                                              >  2:25132/1‑140 (MQ=255)                        cgcCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTa                                                                                                             >  2:183938/1‑140 (MQ=255)                        cgcCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTa                                                                                                             <  1:657594/140‑1 (MQ=255)                           cGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTagag                                                                                                          <  1:466718/140‑1 (MQ=255)                           cGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTagag                                                                                                          <  2:395999/140‑1 (MQ=255)                           cGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTagag                                                                                                          <  1:250915/140‑1 (MQ=255)                                aaCACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCt                                                                                                     <  2:417807/140‑1 (MQ=255)                                aaCACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCt                                                                                                     <  1:559140/140‑1 (MQ=255)                                  cacCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCa                                                                                                   >  1:761980/1‑140 (MQ=255)                                  cacCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCa                                                                                                   >  1:213758/1‑140 (MQ=255)                                       gggTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAAtgct                                                                                              >  1:436998/1‑140 (MQ=255)                                              cTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTcg                                                                                       <  1:25132/140‑1 (MQ=255)                                                 tcgtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCgg                                                                                    >  1:134015/1‑140 (MQ=255)                                                    tcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCt                                                                                 >  1:313026/1‑140 (MQ=255)                                                    tcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCt                                                                                 <  2:134015/140‑1 (MQ=255)                                                       cACCGACTTGAATCCACCGGTAGATTTCCCGGTCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTgg                                                                              <  2:513799/140‑1 (MQ=255)                                                         ccGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGc                                                                            <  2:313026/140‑1 (MQ=255)                                                           gACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCAt                                                                          <  1:183938/140‑1 (MQ=255)                                                            aCTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATc                                                                         <  1:190583/140‑1 (MQ=255)                                                                      aCCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTg                                                               <  2:213758/140‑1 (MQ=255)                                                                      aCCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTg                                                               <  1:244743/140‑1 (MQ=255)                                                                                          aTGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCg                                           <  2:196216/140‑1 (MQ=255)                                                                                           tGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCgg                                          <  2:761980/140‑1 (MQ=255)                                                                                           tGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCgg                                          <  2:436998/140‑1 (MQ=255)                                                                                                       gCGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCa                              >  1:477748/1‑140 (MQ=255)                                                                                                        cGTGATACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCAt                             >  2:37223/1‑140 (MQ=255)                                                                                                            aTACCATGCTCTGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGAc                         >  2:143123/1‑140 (MQ=255)                                                                                                                       tGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAATGCGCCGGAAATCCCGATCATGGACGGCAGcgccg               <  2:397926/139‑1 (MQ=255)                                                                                                                       tGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAATGCGCCGGAAATCCCGATCATGGACGGCAGcgccg               >  1:397926/1‑139 (MQ=255)                                                                                                                       tGTACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGcgccgc              >  2:634958/1‑140 (MQ=255)                                                                                                                         tACGTGTCTTGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTc            <  2:250707/140‑1 (MQ=255)                                                                                                                         tACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTc            <  2:477748/140‑1 (MQ=255)                                                                                                                         tACGTGTCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTc            >  1:84410/1‑140 (MQ=255)                                                                                                                              gtCTGGTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGttt       >  2:369101/1‑140 (MQ=255)                                                                                                                                   gTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGtata  >  1:702881/1‑140 (MQ=255)                                                                                                                                   gTCAGCGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGtata  >  2:196384/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        AGTCACCCTGACGTTACGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATA  >  NC_000913/106625‑106894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑