Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,893,898	C→A	G414G (GGC→GGA)	ygcN →	putative oxidoreductase with FAD/NAD(P)‑binding domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,893,898	0	C	A	100.0%	150.1 / NA	44	G414G (GGC→GGA)	ygcN	putative oxidoreductase with FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/22);  total (22/22)

GATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCT  >  NC_000913/2893762‑2894031                                                                                                                                         |                                                                                                                                      gATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGAt                                                                                                                                    <  2:480022/140‑1 (MQ=255)       aCCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGAt                                                                                                                                    >  2:63710/1‑134 (MQ=255)       aCCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGAt                                                                                                                                    <  1:63710/134‑1 (MQ=255)           tACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGa                                                                                                                          >  1:849740/1‑140 (MQ=255)           tACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGa                                                                                                                          >  1:110034/1‑140 (MQ=255)           tACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGa                                                                                                                          >  2:816549/1‑140 (MQ=255)           tACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGa                                                                                                                          >  2:549655/1‑140 (MQ=255)                ggCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTg                                                                                                                     >  2:278498/1‑140 (MQ=255)                ggCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTg                                                                                                                     >  2:302646/1‑140 (MQ=255)                ggCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTg                                                                                                                     >  1:758068/1‑140 (MQ=255)                 gCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGc                                                                                                                    <  1:575063/140‑1 (MQ=255)                    tGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGAt                                                                                                                 <  1:816549/140‑1 (MQ=255)                      cGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGt                                                                                                               >  2:222170/1‑140 (MQ=255)                      cGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGt                                                                                                               >  1:650749/1‑140 (MQ=255)                          aaTGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGt                                                                                                           >  2:616846/1‑140 (MQ=255)                            tGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGc                                                                                                                    >  2:806957/1‑129 (MQ=255)                            tGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGc                                                                                                                    <  1:806957/129‑1 (MQ=255)                            tGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAg                                                                                                         <  2:756715/140‑1 (MQ=255)                                ggATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCg                                                                                                     >  1:312253/1‑140 (MQ=255)                                   tatTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTaa                                                                                                  >  1:566300/1‑140 (MQ=255)                                          cgcgATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTgg                                                                                           <  2:849740/140‑1 (MQ=255)                                           gcgATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGgc                                                                                          >  2:473854/1‑140 (MQ=255)                                            cgATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGgcg                                                                                         <  2:432469/140‑1 (MQ=255)                                                    gggATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTg                                                                                 <  2:566300/140‑1 (MQ=255)                                                    gggATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTg                                                                                 <  2:110034/140‑1 (MQ=255)                                                         cAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATgcg                                                                            <  1:616846/140‑1 (MQ=255)                                                         cAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCATTGCTGATgcg                                                                            <  1:473854/140‑1 (MQ=255)                                                                aTAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGcgcc                                                                          <  2:262074/135‑1 (MQ=255)                                                                aTAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGcgcc                                                                          >  1:262074/1‑135 (MQ=255)                                                                aTAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGcaca                                                                     >  2:758353/1‑140 (MQ=255)                                                                aTAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGcaca                                                                     <  1:278498/140‑1 (MQ=255)                                                                     ccTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGtt                                                                <  2:147556/140‑1 (MQ=255)                                                                        gTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCg                                                             >  1:780244/1‑140 (MQ=255)                                                                                            aTTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCa                                         <  2:312253/140‑1 (MQ=255)                                                                                            aTTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCa                                         <  1:549655/140‑1 (MQ=255)                                                                                             ttCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAg                                        <  1:758353/140‑1 (MQ=255)                                                                                                       atTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAAc                              >  2:331076/1‑140 (MQ=255)                                                                                                         ttACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAACGCCAGACGGCAGAACgg                            <  2:841726/140‑1 (MQ=255)                                                                                                          tACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGt                           <  2:758068/140‑1 (MQ=255)                                                                                                          tACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGt                           <  1:302646/140‑1 (MQ=255)                                                                                                                  cACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAAc                   >  1:852006/1‑140 (MQ=255)                                                                                                                           tgGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCg          >  1:125761/1‑140 (MQ=255)                                                                                                                                 cTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGt    <  2:780244/140‑1 (MQ=255)                                                                                                                                 cTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGt    >  1:387921/1‑140 (MQ=255)                                                                                                                                 cTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTCGATTAACGCCTGTCCGGCAggc    >  2:473427/1‑139 (MQ=255)                                                                                                                                   ggcgggagATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGCCt  >  1:533108/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      GATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCT  >  NC_000913/2893762‑2894031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑