Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,261,710	C→A	S14R (AGC→AGA)	dusA →	tRNA‑dihydrouridine synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,261,710	0	C	A	100.0%	259.5 / NA	73	S14R (AGC→AGA)	dusA	tRNA‑dihydrouridine synthase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (43/30);  total (43/30)

TATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGT  >  NC_000913/4261575‑4261844                                                                                                                                        |                                                                                                                                       tATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCaa                                                                                                                                    >  2:109180/1‑140 (MQ=255)  aTGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAAt                                                                                                                                   >  1:348504/1‑140 (MQ=255)        taGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGa                                                                                                                             >  1:795906/1‑140 (MQ=255)          gTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaa                                                                                                                           >  1:652641/1‑140 (MQ=255)               ccGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACt                                                                                                                      >  1:491345/1‑140 (MQ=255)                cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaaaaa                                                                                                                        >  2:490404/1‑137 (MQ=255)                cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaaaaa                                                                                                                        <  1:490404/137‑1 (MQ=255)                cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                     >  2:9905/1‑140 (MQ=255)                cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                     >  1:703425/1‑140 (MQ=255)                cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                     >  2:250441/1‑140 (MQ=255)                cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                     >  1:351976/1‑140 (MQ=255)                  cTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAGCCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                   <  2:340678/140‑1 (MQ=255)                   tATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACg                                                                                                                  <  1:9905/140‑1 (MQ=255)                     ttGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGtt                                                                                                                >  2:678967/1‑140 (MQ=255)                     ttGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGtt                                                                                                                >  1:185348/1‑140 (MQ=255)                       gAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCa                                                                                                              <  2:302712/140‑1 (MQ=255)                       gAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCa                                                                                                              <  2:703425/140‑1 (MQ=255)                          cctcctGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTg                                                                                                           >  2:447844/1‑140 (MQ=255)                             cctGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAg                                                                                                        >  2:568922/1‑140 (MQ=255)                             cctGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAg                                                                                                        >  2:285909/1‑140 (MQ=255)                             cctGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAg                                                                                                        >  1:244211/1‑140 (MQ=255)                               tGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTg                                                                                                      >  1:487382/1‑140 (MQ=255)                                 aaTAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGt                                                                                                    <  2:351976/140‑1 (MQ=255)                                 aaTAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGt                                                                                                    <  2:487382/140‑1 (MQ=255)                                 aaTAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGt                                                                                                    <  1:14905/140‑1 (MQ=255)                                      tGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGtt                                                                                               <  2:546654/140‑1 (MQ=255)                                        aTGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTa                                                                                             >  2:341436/1‑140 (MQ=255)                                        aTGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTa                                                                                             >  2:239976/1‑140 (MQ=255)                                         tGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAg                                                                                            <  1:447844/140‑1 (MQ=255)                                           cTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCg                                                                                          >  2:460584/1‑140 (MQ=255)                                                      aacCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATg                                                                               <  1:678967/140‑1 (MQ=255)                                                        ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCt                                                                             >  1:529204/1‑140 (MQ=255)                                                        ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGATAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCt                                                                             >  2:559886/1‑140 (MQ=255)                                                          ccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCg                                                                           >  2:273500/1‑140 (MQ=255)                                                             tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACt                                                                        >  2:110386/1‑140 (MQ=255)                                                             tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACt                                                                        >  2:159861/1‑140 (MQ=255)                                                             tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACt                                                                        >  1:566561/1‑140 (MQ=255)                                                              gATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGCGGTCGGTTTAGCGTTGCACCAATGCTCGACTg                                                                       >  1:687972/1‑140 (MQ=255)                                                               atatatCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTgg                                                                      <  2:491345/140‑1 (MQ=255)                                                                   atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                  >  1:135047/1‑140 (MQ=255)                                                                   atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                  >  1:293444/1‑140 (MQ=255)                                                                   atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                  >  1:760923/1‑140 (MQ=255)                                                                   atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                  >  2:174883/1‑140 (MQ=255)                                                                       aTAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacag                                                              >  2:495462/1‑140 (MQ=255)                                                                       aTAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacag                                                              >  1:594816/1‑140 (MQ=255)                                                                                    tACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTAt                                                 >  2:224576/1‑140 (MQ=255)                                                                                    tACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTAt                                                 >  1:770090/1‑140 (MQ=255)                                                                                     aCATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTAtt                                                >  1:281211/1‑140 (MQ=255)                                                                                       aTTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTc                                              <  1:495462/140‑1 (MQ=255)                                                                                         ttGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCtt                                            >  2:62185/1‑140 (MQ=255)                                                                                          tGAAAATGCACGGTAATTCTGAAATGTAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTg                                           >  2:254605/1‑140 (MQ=255)                                                                                           gAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGc                                          >  1:803501/1‑140 (MQ=255)                                                                                           gAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGc                                          <  1:772313/140‑1 (MQ=255)                                                                                              aaTGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCGGCTATTTCTTGCgcc                                       <  2:687972/140‑3 (MQ=255)                                                                                                  cACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCt                                   <  2:739772/140‑1 (MQ=255)                                                                                                   aCGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCtt                                  <  2:760923/140‑1 (MQ=255)                                                                                                          ttCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCa                           <  2:410416/140‑1 (MQ=255)                                                                                                          ttCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCa                           <  1:568922/140‑1 (MQ=255)                                                                                                             tGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATa                        <  2:803501/140‑1 (MQ=255)                                                                                                             tGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATa                        <  1:159861/140‑1 (MQ=255)                                                                                                             tGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATa                        <  2:281211/140‑1 (MQ=255)                                                                                                               aaaTGTAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      >  2:819943/1‑140 (MQ=255)                                                                                                               aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      <  1:224576/140‑1 (MQ=255)                                                                                                               aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      <  1:285909/140‑1 (MQ=255)                                                                                                                 aTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGtt                    >  2:467428/1‑140 (MQ=255)                                                                                                                  tgtaaaaaaTCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                   <  1:254605/137‑1 (MQ=255)                                                                                                                  tGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                   <  2:652641/140‑1 (MQ=255)                                                                                                                    cAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCt                 <  2:529204/140‑1 (MQ=255)                                                                                                                    cAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCt                 >  1:675909/1‑140 (MQ=255)                                                                                                                       aaaaTCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGta              <  2:675909/140‑1 (MQ=255)                                                                                                                              accaaaccaGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGaa       >  2:502269/1‑140 (MQ=255)                                                                                                                               ccaaaccaGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGaaa      <  1:460584/140‑1 (MQ=255)                                                                                                                                   accaGACCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGt  <  2:630085/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       TATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGT  >  NC_000913/4261575‑4261844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑