Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,405,258	C→A	T191K (ACG→AAG)	purA →	adenylosuccinate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,405,258	0	C	A	100.0%	179.6 / NA	51	T191K (ACG→AAG)	purA	adenylosuccinate synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (26/25);  total (26/25)

TTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTAT  >  NC_000913/4405132‑4405394                                                                                                                               |                                                                                                                                         ttGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGcc                                                                                                                             <  2:22768/140‑1 (MQ=255)       aCCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGcc                                                                                                                             >  1:67716/1‑134 (MQ=255)       aCCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGcc                                                                                                                             <  2:67716/134‑1 (MQ=255)         cTTTTCGACAAAGAATCCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACCTCTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGAGAAGATGGCTGTTGCCGACATcgt                                                                                                                     <  2:745156/140‑3 (MQ=255)           tttCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGa                                                                                                                   >  2:161436/1‑140 (MQ=255)                           ttCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGa                                                                                                   <  1:161436/140‑1 (MQ=255)                           ttCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGa                                                                                                   <  2:439544/140‑1 (MQ=255)                              gCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGt                                                                                                >  2:611709/1‑140 (MQ=255)                              gCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGt                                                                                                >  1:144245/1‑140 (MQ=255)                                    aaaCTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGa                                                                                          >  1:643290/1‑140 (MQ=255)                                           aagaagTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTc                                                                                   <  2:553337/140‑1 (MQ=255)                                                       aaTATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGt                                                                       <  1:611709/140‑1 (MQ=255)                                                           tCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGc                                                                   >  1:194329/1‑140 (MQ=255)                                                             acaACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGt                                                                 >  1:735005/1‑140 (MQ=255)                                                                aCTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGc                                                              >  2:411000/1‑140 (MQ=255)                                                                 cTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCg                                                             >  1:554362/1‑140 (MQ=255)                                                                       gTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCg                                                       >  1:290254/1‑140 (MQ=255)                                                                         tGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTc                                                     <  1:241185/140‑1 (MQ=255)                                                                           gTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTgg                                                               >  1:9059/1‑128 (MQ=255)                                                                           gTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTgg                                                               <  2:9059/128‑1 (MQ=255)                                                                            ttAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGAt                                                           >  1:508270/1‑131 (MQ=255)                                                                            ttAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGAt                                                           <  2:508270/131‑1 (MQ=255)                                                                              aaCTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGtt                                                <  2:290254/140‑1 (MQ=255)                                                                                   ctacAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAg                                           <  2:643290/140‑1 (MQ=255)                                                                                     acAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGt                                         >  1:246879/1‑140 (MQ=255)                                                                                      cAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTg                                        >  1:239695/1‑140 (MQ=255)                                                                                       aaaGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTgc                                       <  2:246879/140‑1 (MQ=255)                                                                                         aGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTgcgc                                     >  2:555083/1‑140 (MQ=255)                                                                                         aGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTgcgc                                     >  1:834918/1‑140 (MQ=255)                                                                                         aGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTgcgc                                     >  2:527745/1‑140 (MQ=255)                                                                                         aGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTgcgc                                     >  1:384153/1‑140 (MQ=255)                                                                                             gAAGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAggg                                 <  2:144245/140‑1 (MQ=255)                                                                                              aaGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTgc                                       >  1:339769/1‑133 (MQ=255)                                                                                              aaGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTgc                                       <  2:339769/133‑1 (MQ=255)                                                                                               aGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTa                               <  1:527745/140‑1 (MQ=255)                                                                                               aGCGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTa                               <  1:96966/140‑1 (MQ=255)                                                                                                 cGGTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACg                             <  1:284125/140‑1 (MQ=255)                                                                                                   gTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTa                               <  2:168392/136‑1 (MQ=255)                                                                                                   gTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTa                               >  1:168392/1‑136 (MQ=255)                                                                                                   gTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACgct                           >  2:393518/1‑140 (MQ=255)                                                                                                   gTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACgct                           >  1:440248/1‑140 (MQ=255)                                                                                                   gTTGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTAT‑GTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACgctg                          >  2:174629/1‑140 (MQ=255)                                                                                                     tGATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACgctgc                         >  1:521122/1‑140 (MQ=255)                                                                                                      gATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACgctgct                        <  1:408777/140‑1 (MQ=255)                                                                                                      gATTACCAGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACgctgct                        >  1:437164/1‑140 (MQ=255)                                                                                                             aGAAAGTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATc                 <  1:411000/140‑1 (MQ=255)                                                                                                                  gTTCTGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCa            <  2:735005/140‑1 (MQ=255)                                                                                                                      tGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGt        >  2:805002/1‑140 (MQ=255)                                                                                                                      tGGATGATAAGATGGCTGTTGCCGACATCCTGACTTCTAT‑GTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTa       <  1:174629/140‑1 (MQ=255)                                                                                                                            atAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTAt  <  1:828134/140‑1 (MQ=255)                                                                                                                            atAAGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTAt  <  1:608021/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                         TTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTAT  >  NC_000913/4405132‑4405394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑