Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	414,763	G→T	R331S (CGC→AGC)	sbcC ←	ATP dependent, structure specific DNA nuclease ‑ SbcC subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	414,763	0	G	T	100.0%	162.9 / NA	46	R331S (CGC→AGC)	sbcC	ATP dependent, structure specific DNA nuclease ‑ SbcC subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (26/20);  total (26/20)

GGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGT  >  NC_000913/414635‑414893                                                                                                                                 |                                                                                                                                   ggAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTcgcgc                                                                                                                         >  1:229512/1‑140 (MQ=255)   agaACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGa                                                                                                                       >  1:554848/1‑140 (MQ=255)       cgccccACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAgcg                                                                                                                   <  2:229512/135‑1 (MQ=255)        tGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAgcgc                                                                                                                  >  2:831582/1‑140 (MQ=255)          cgcACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCa                                                                                                                >  1:631541/1‑140 (MQ=255)          cgcACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCa                                                                                                                <  2:554848/140‑1 (MQ=255)               gCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATtgtg                                                                                                           <  1:831582/140‑1 (MQ=255)                 cAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATtgtg                                                                                                           <  1:215679/138‑1 (MQ=255)                 cAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATtgtg                                                                                                           >  2:215679/1‑138 (MQ=255)                  aaCCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGctc                                                                                                        >  1:59884/1‑140 (MQ=255)                        ccgGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAg                                                                                                  <  2:59884/140‑1 (MQ=255)                         cgGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGc                                                                                                 >  1:240088/1‑140 (MQ=255)                              cGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTa                                                                                            <  2:240088/140‑1 (MQ=255)                                     ccACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTActt                                                                                     >  2:15951/1‑140 (MQ=255)                                        cTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTActtctt                                                                                  >  2:330195/1‑140 (MQ=255)                                           aCGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCaa                                                                               >  2:445455/1‑140 (MQ=255)                                                          ttCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGc                                                                      >  2:773287/1‑134 (MQ=255)                                                          ttCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGc                                                                      <  1:773287/134‑1 (MQ=255)                                                                    cAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAgcgc                                                      >  2:11887/1‑140 (MQ=255)                                                                       gTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGcgccgc                                                   >  1:298673/1‑140 (MQ=255)                                                                        tATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCcgcg                                                  <  1:609971/140‑1 (MQ=255)                                                                          ttCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCt                                                <  1:684998/140‑1 (MQ=255)                                                                          ttCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCt                                                <  1:601894/140‑1 (MQ=255)                                                                          ttCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCt                                                <  1:445455/140‑1 (MQ=255)                                                                          ttCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCt                                                <  2:631541/140‑1 (MQ=255)                                                                            cAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCtgt                                              >  2:419554/1‑140 (MQ=255)                                                                            cAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCtgt                                              >  2:261638/1‑140 (MQ=255)                                                                            cAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCtgt                                              >  1:303857/1‑140 (MQ=255)                                                                                   tgttgCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGc                                       <  1:11887/140‑1 (MQ=255)                                                                                     ttgCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGa                                     >  1:426524/1‑140 (MQ=255)                                                                                     ttgCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGa                                     <  2:303857/140‑1 (MQ=255)                                                                                     ttgCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGa                                     <  1:800362/140‑1 (MQ=255)                                                                                       gctgctgctGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATg                                   <  1:261638/140‑1 (MQ=255)                                                                                       gctgctgctGTAATTCTGCTGACTGCGTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTACGATg                                   <  2:207371/140‑1 (MQ=255)                                                                                           ctgctgTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGtt                               <  1:419554/140‑1 (MQ=255)                                                                                                gTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAg                          <  1:330195/140‑1 (MQ=255)                                                                                                        gctgACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACg                  >  1:561397/1‑140 (MQ=255)                                                                                                         ctgACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGa                 >  2:594544/1‑140 (MQ=255)                                                                                                           gACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTcc                             <  2:90941/126‑1 (MQ=255)                                                                                                           gACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTcc                             >  1:90941/1‑126 (MQ=255)                                                                                                           gACTGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAg               >  1:343262/1‑140 (MQ=255)                                                                                                              tGCTTCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGAtt            >  1:614651/1‑140 (MQ=255)                                                                                                                cTTCGCCGCGTGGTGGCTAATGCTCACGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTc          >  1:313646/1‑140 (MQ=255)                                                                                                                 ttCGCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCg         >  2:210866/1‑140 (MQ=255)                                                                                                                      cgcgTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCg    >  1:353710/1‑140 (MQ=255)                                                                                                                       gcgTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCgg   <  1:210866/140‑1 (MQ=255)                                                                                                                        cgTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGt  >  2:628228/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                   GGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGT  >  NC_000913/414635‑414893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑