Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,085,013	C→A	E171D (GAG→GAT)	yeeE ←	inner membrane protein YeeE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,085,013	0	C	A	100.0%	340.9 / NA	96	E171D (GAG→GAT)	yeeE	inner membrane protein YeeE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (47/49);  total (47/49)

GGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATG  >  NC_000913/2084879‑2085147                                                                                                                                       |                                                                                                                                       ggCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCttt                                                                                                                                   >  1:1164701/1‑140 (MQ=255)     aGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTtcatc                                                                                                                               >  1:649139/1‑140 (MQ=255)      ggCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTtcatca                                                                                                                              <  2:1746516/140‑1 (MQ=255)      ggCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTtcatca                                                                                                                              >  1:1468136/1‑140 (MQ=255)      ggCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTtcatca                                                                                                                              >  2:1267568/1‑140 (MQ=255)      ggCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTtcatca                                                                                                                              >  1:1370046/1‑140 (MQ=255)       gCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATcac                                                                                                                             <  2:458853/140‑1 (MQ=255)       gCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATcac                                                                                                                             <  2:1164701/140‑1 (MQ=255)          aaaaGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATcaccac                                                                                                                          >  2:1256541/1‑140 (MQ=255)            aaGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCAccc                                                                                                                        >  1:541099/1‑140 (MQ=255)            aaGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCAccc                                                                                                                        <  2:719779/140‑1 (MQ=255)            aaGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCAccc                                                                                                                        >  2:1523138/1‑140 (MQ=255)              gcgcAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCag                                                                                                                      <  1:1267568/140‑1 (MQ=255)              gcgcAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCag                                                                                                                      <  1:377198/140‑1 (MQ=255)                 cAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTtcatca                                                                                                                              <  2:1735220/129‑1 (MQ=255)                 cAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTtcatca                                                                                                                              >  1:1735220/1‑129 (MQ=255)                 cAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGc                                                                                                                   >  2:773127/1‑140 (MQ=255)                 cAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGc                                                                                                                   >  1:186225/1‑140 (MQ=255)                 cAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGc                                                                                                                   >  1:506770/1‑140 (MQ=255)                        aCCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCa                                                                                                            >  1:1172446/1‑140 (MQ=255)                                 tACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCaaaa                                                                                                   >  1:636290/1‑140 (MQ=255)                                 tACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCaaaa                                                                                                   >  1:1043581/1‑140 (MQ=255)                                         tgACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGcaacc                                                                                           >  2:17272/1‑140 (MQ=255)                                         tgACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGcaacc                                                                                           <  2:186225/140‑1 (MQ=255)                                           aCAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGcaaccaa                                                                                         >  2:395875/1‑140 (MQ=255)                                           aCAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGcaaccaa                                                                                         >  1:1318078/1‑140 (MQ=255)                                           aCAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGGCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGcaaccaa                                                                                         >  2:853121/1‑140 (MQ=255)                                             aaaGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACg                                                                                       <  1:1139991/140‑1 (MQ=255)                                                gggATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGcc                                                                                    >  2:1612255/1‑140 (MQ=255)                                                    tGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCcacac                                                                                >  2:1171026/1‑140 (MQ=255)                                                     gCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCcacaca                                                                               <  1:62399/140‑1 (MQ=255)                                                     gCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCcacaca                                                                               >  1:1044047/1‑140 (MQ=255)                                                      ccAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAg                                                                              >  2:594370/1‑140 (MQ=255)                                                       cAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGa                                                                             <  2:1044047/140‑1 (MQ=255)                                                       cAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGa                                                                             <  1:594370/140‑1 (MQ=255)                                                           gTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCcaca                                                                                 <  2:1111719/132‑1 (MQ=255)                                                           gTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCcaca                                                                                 >  1:1111719/1‑132 (MQ=255)                                                           gTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACaaa                                                                         >  1:1450005/1‑140 (MQ=255)                                                               ttCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTaa                                                                     <  2:1091330/140‑1 (MQ=255)                                                               ttCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTaa                                                                     >  1:700198/1‑140 (MQ=255)                                                                 cAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTaaaa                                                                   <  1:17272/140‑1 (MQ=255)                                                                   aaCAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGt                                                                 <  1:773127/140‑1 (MQ=255)                                                                      aGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGtctc                                                              <  1:940627/140‑1 (MQ=255)                                                                      aGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGtctc                                                              <  2:700198/140‑1 (MQ=255)                                                                       gAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCa                                                             >  2:1691996/1‑140 (MQ=255)                                                                        aaTATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAg                                                            >  2:1556420/1‑140 (MQ=255)                                                                        aaTATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAg                                                            <  2:1341102/140‑1 (MQ=255)                                                                        aaTATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAg                                                            >  2:941039/1‑140 (MQ=255)                                                                           atGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCaa                                                         <  1:396337/140‑1 (MQ=255)                                                                              aGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATag                                                      >  2:497396/1‑140 (MQ=255)                                                                                cGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATagag                                                    <  2:1172446/140‑1 (MQ=255)                                                                                cGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATagag                                                    >  1:109922/1‑140 (MQ=255)                                                                                  aTCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGtt                                                  <  2:986/140‑1 (MQ=255)                                                                                  aTCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGtt                                                  <  2:1517107/140‑1 (MQ=255)                                                                                  aTCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGtt                                                  >  1:864526/1‑140 (MQ=255)                                                                                  aTCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGtt                                                  >  1:1517107/1‑140 (MQ=255)                                                                                  aTCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGtt                                                  <  1:1724878/140‑1 (MQ=255)                                                                                  aTCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGtt                                                  >  1:52175/1‑140 (MQ=255)                                                                                  aTCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGtt                                                  >  1:253722/1‑140 (MQ=255)                                                                                  aTCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGtt                                                  >  2:1724878/1‑140 (MQ=255)                                                                                    cccGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTAt                                                <  1:963176/140‑1 (MQ=255)                                                                                    cccGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTAt                                                >  2:537253/1‑140 (MQ=255)                                                                                    cccGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTAt                                                >  1:515386/1‑140 (MQ=255)                                                                                       ggTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGtt                                             <  1:902319/140‑1 (MQ=255)                                                                                       ggTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGtt                                             <  2:1318078/140‑1 (MQ=255)                                                                                       ggTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGtt                                             <  1:1171026/140‑1 (MQ=255)                                                                                       ggTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGtt                                             <  2:52175/140‑1 (MQ=255)                                                                                       ggTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGtt                                             <  1:756878/140‑1 (MQ=255)                                                                                       ggTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGtt                                             <  1:614252/140‑1 (MQ=255)                                                                                       ggTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGtt                                             <  1:1523138/140‑1 (MQ=255)                                                                                       ggTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGtt                                             <  1:395875/140‑1 (MQ=255)                                                                                       ggTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCAAACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGtt                                             <  2:1182570/140‑1 (MQ=255)                                                                                        gTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTc                                            >  1:282336/1‑140 (MQ=255)                                                                                        gTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTc                                            <  1:293473/140‑1 (MQ=255)                                                                                         ttCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCa                                           <  2:649139/140‑1 (MQ=255)                                                                                          tCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAg                                          <  1:348548/140‑1 (MQ=255)                                                                                            ggcgcggcgGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCATCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTa                                        <  2:1566927/140‑1 (MQ=255)                                                                                              cgcggcgGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATa                                                       >  1:990226/1‑123 (MQ=255)                                                                                              cgcggcgGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATa                                                       <  2:990226/123‑1 (MQ=255)                                                                                               gcggcgGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTg                                     <  1:853121/140‑1 (MQ=255)                                                                                                  gcggTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAg                                  <  1:497396/140‑1 (MQ=255)                                                                                                         ggTCGCGACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCa                           <  2:1450005/140‑1 (MQ=255)                                                                                                             gcgACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGt                       >  1:846745/1‑140 (MQ=255)                                                                                                               gACTTTGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGttt                     >  1:573363/1‑140 (MQ=255)                                                                                                                   ttGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGAtt                 <  2:506770/140‑1 (MQ=255)                                                                                                                    tGAGTTTTGGCTTCTTCAGATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGAttt                >  1:1024189/1‑140 (MQ=255)                                                                                                                    tGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGAttt                >  1:772623/1‑140 (MQ=255)                                                                                                                    tGAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGAttt                >  1:647118/1‑140 (MQ=255)                                                                                                                     gAGTTTTGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTa               <  2:647118/140‑1 (MQ=255)                                                                                                                          ttGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCa          <  1:1009419/140‑1 (MQ=255)                                                                                                                          ttGGCTTCTTCAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCa          <  1:537253/140‑1 (MQ=255)                                                                                                                               ttcttcAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGc     <  2:109922/140‑1 (MQ=255)                                                                                                                               ttcttcAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGc     <  1:1256541/140‑1 (MQ=255)                                                                                                                               ttcttcAAATCTTTCATCACCACCCAGAACGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGc     <  2:282336/140‑1 (MQ=255)                                                                                                                                tcttcAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCa    >  2:1215709/1‑140 (MQ=255)                                                                                                                                  ttcAAATCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCAtg  >  1:1025172/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       GGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATG  >  NC_000913/2084879‑2085147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑