Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,811,850	G→T	D142Y (GAC→TAC)	emrA →	multidrug efflux pump membrane fusion protein EmrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,811,850	0	G	T	100.0%	229.4 / NA	67	D142Y (GAC→TAC)	emrA	multidrug efflux pump membrane fusion protein EmrA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (34/33);  total (34/33)

GCTCGCCAGGCGTTTGAAAAAGCCAAAACTGCACTGGCTTCCAGCGTTCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCGACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCAGGCG  >  NC_000913/2811715‑2811984                                                                                                                                        |                                                                                                                                       gCTCGCCAGGCGTTTGAAAAAGCCAAAACTGCACTGGCTTCCAGCGTTCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGctacta                                                                                                                                    <  1:760911/140‑1 (MQ=255)     gCCAGGCGTTTGAAAAAGCCAAAACTGCACTGGCTTCCAGCGTTCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAAc                                                                                                                                <  1:650409/140‑1 (MQ=255)         ggCGTTTGAAAAAGCCAAAACTGCACTGGCTTCCAGCGTTCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAACACAAAGCTACTACAAccgcc                                                                                                                            <  1:1396206/140‑1 (MQ=255)           cGTTTGAAAAAGCCAAAACTGCACTGGCTTCCAGCGTTCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCgt                                                                                                                          >  2:1525121/1‑140 (MQ=255)            gTTTGAAAAAGCCAAAACTGCACTGGCTTCCAGCGTTCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCgtg                                                                                                                         <  2:552592/140‑1 (MQ=255)               tGAAAAAGCCAAAACTGCACTGGCTTCCAGCGTTCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGc                                                                                                                      >  1:627662/1‑140 (MQ=255)                 aaaaaGCCAAAACTGCACTGGCTTCCAGCGTTCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGtgtg                                                                                                                       >  1:31991/1‑137 (MQ=255)                 aaaaaGCCAAAACTGCACTGGCTTCCAGCGTTCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGtgtg                                                                                                                       >  1:339433/1‑137 (MQ=255)                 aaaaaGCCAAAACTGCACTGGCTTCCAGCGTTCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGtgtg                                                                                                                       <  2:31991/137‑1 (MQ=255)                 aaaaaGCCAAAACTGCACTGGCTTCCAGCGTTCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGtgtg                                                                                                                       <  2:339433/137‑1 (MQ=255)                  aaaaGCCAAAACTGCACTGGCTTCCAGCGTTCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGc                                                                                                                   <  1:1568844/140‑1 (MQ=255)                    aaGCCAAAACTGCAATGGCTTCCAGCGTTCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTg                                                                                                                 >  1:639164/1‑140 (MQ=255)                     aGCCAAAACTGCACTGGCTTCCAGCGTTCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTgg                                                                                                                >  1:1268994/1‑140 (MQ=255)                      gCCAAAACTGCACTGGCTTCCAGCGTTCGCCAACCCCCCCAGTTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTggg                                                                                                               <  1:38812/140‑1 (MQ=255)                      gCCAAAACTGCACTGGCTTCCAGCGTTCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTggg                                                                                                               <  2:1268994/140‑1 (MQ=255)                        cAAAACTGCATTGGCTTCCAGCTTTCGCAAAGCCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGGGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCa                                                                                                             <  2:188255/140‑1 (MQ=255)                                cACTGGCTTCCAGCGTTCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAAc                                                                                                     >  2:1734511/1‑140 (MQ=255)                                cACTGGCTTCCAGCGTTCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAAc                                                                                                     >  1:121927/1‑140 (MQ=255)                                cACTGGCTTCCAGCGTTCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAAc                                                                                                     >  1:171838/1‑140 (MQ=255)                                        tCCAGCGTTCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTgg                                                                                             >  1:399496/1‑140 (MQ=255)                                        tCCAGCGTTCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTgg                                                                                             <  1:1734511/140‑1 (MQ=255)                                               ttCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGaa                                                                                      >  1:44716/1‑140 (MQ=255)                                               ttCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGaa                                                                                      >  2:1153183/1‑140 (MQ=255)                                               ttCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGaa                                                                                      >  1:264463/1‑140 (MQ=255)                                                tCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAag                                                                                     <  2:627662/140‑1 (MQ=255)                                                tCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAag                                                                                     >  1:1416950/1‑140 (MQ=255)                                                   ccAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGc                                                                                  <  1:302287/140‑1 (MQ=255)                                                                  gatgatTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCGACCTGATTGGTCGCGAAGAGCTGCAACACGCCcgcg                                                                   <  1:1633847/140‑1 (MQ=255)                                                                   atgatTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGa                                                                  >  1:889344/1‑140 (MQ=255)                                                                   atgatTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGa                                                                  >  1:1648908/1‑140 (MQ=255)                                                                   atgatTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGa                                                                  >  2:1736143/1‑140 (MQ=255)                                                                    tgatTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGAc                                                                 <  2:639164/140‑1 (MQ=255)                                                                    tgatTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGAc                                                                 <  1:1153183/140‑1 (MQ=255)                                                                    tgatTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGAc                                                                 <  2:171838/140‑1 (MQ=255)                                                                    tgatTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGAc                                                                 <  2:1416950/140‑1 (MQ=255)                                                                    tgatTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAAACGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGAc                                                                 <  1:952071/140‑1 (MQ=255)                                                                     gatTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACg                                                                >  1:1484954/1‑140 (MQ=255)                                                                       ttAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGcc                                                              <  1:1525121/140‑1 (MQ=255)                                                                         aaCAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGt                                                            <  2:61187/140‑1 (MQ=255)                                                                            agcaagcaGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCAc                                                         <  2:121927/140‑1 (MQ=255)                                                                              caagcaGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCa                                                       <  1:142984/140‑1 (MQ=255)                                                                                agcaGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGt                                                     >  2:1105827/1‑140 (MQ=255)                                                                                agcaGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGt                                                     >  2:1229860/1‑140 (MQ=255)                                                                                agcaGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGt                                                     >  1:534828/1‑140 (MQ=255)                                                                                    gTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGccc                                                 <  2:1648908/140‑1 (MQ=255)                                                                                     ttGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCa                                                >  2:1233394/1‑140 (MQ=255)                                                                                       gCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAgg                                              <  2:399496/140‑1 (MQ=255)                                                                                             gAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAAc                                        >  1:14704/1‑140 (MQ=255)                                                                                              aaTATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAACt                                       <  1:1397882/140‑1 (MQ=255)                                                                                               atatTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAACTg                                      >  2:1722083/1‑140 (MQ=255)                                                                                                     aGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAACTGGACGTc                                >  1:945840/1‑140 (MQ=255)                                                                                                     aGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAACTGGACGTc                                >  1:1517924/1‑140 (MQ=255)                                                                                                      ggTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAACTGGACGTcg                               <  1:1736143/140‑1 (MQ=255)                                                                                                       gTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAgg                                              <  2:1071547/124‑1 (MQ=255)                                                                                                       gTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAgg                                              >  1:1071547/1‑124 (MQ=255)                                                                                                         gCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAACTGGACGTCGCGa                            <  2:14704/140‑1 (MQ=255)                                                                                                         gCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAACTGGACGTCGCGa                            <  1:1309166/140‑1 (MQ=255)                                                                                                         gCAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAACTGGACGTCGCGa                            <  2:44716/140‑1 (MQ=255)                                                                                                          cAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAACTGGACGTCGCGa                            <  1:429686/139‑1 (MQ=255)                                                                                                          cAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAACTGGACGTCGCGa                            >  2:429686/1‑139 (MQ=255)                                                                                                          cAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAACTGGACGTCGCGAt                           >  1:1134379/1‑140 (MQ=255)                                                                                                          cAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAACTGGACGTCGCGAt                           >  1:712783/1‑140 (MQ=255)                                                                                                          cAGAAAATCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAACTGGACGTCGCGAt                           >  2:589882/1‑140 (MQ=255)                                                                                                              aaaTCGCCCTCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAACTGGACGTCGCGATTcaa                       <  1:589882/140‑1 (MQ=255)                                                                                                                       tCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAACTGGACGTCGCGATTCAacaatacaat              >  2:816404/1‑140 (MQ=255)                                                                                                                       tCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAACTGGACGTCGCGATTCAacaatacaat              <  1:1105827/140‑1 (MQ=255)                                                                                                                       tCGCGAAAGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAACTGGACGTCGCGATTCAacaatacaat              >  2:1594039/1‑140 (MQ=255)                                                                                                                              aGCACAAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATc       >  1:611278/1‑140 (MQ=255)                                                                                                                               ccacaAAGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCa      <  2:1392952/139‑1 (MQ=255)                                                                                                                                   aaaGCTACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCAGGCg  <  2:945840/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       GCTCGCCAGGCGTTTGAAAAAGCCAAAACTGCACTGGCTTCCAGCGTTCGCCAAACCCACCAGCTGATGATTAACAGCAAGCAGTTGCAGGCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCGACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCAGGCG  >  NC_000913/2811715‑2811984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑