Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	595,933	G→T	G112C (GGT→TGT)	cusC →	copper/silver export system outer membrane channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	595,933	0	G	T	100.0%	233.8 / NA	69	G112C (GGT→TGT)	cusC	copper/silver export system outer membrane channel
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (39/30);  total (39/30)

TGATTAGCGAGGCGCTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGACAAAATTATTTAGCCACTGAGGAAGC  >  NC_000913/595796‑596069                                                                                                                                          |                                                                                                                                         tGATTAGCGAGGCGCTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGATCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGt                                                                                                                                        >  2:745453/1‑140 (MQ=255) tGATTAGCGAGGCGCTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGt                                                                                                                                        >  1:637057/1‑140 (MQ=255)  gATTAGCGAGGCGCTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTa                                                                                                                                       <  2:967032/140‑1 (MQ=255)  gATTAGCGAGGCGCTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTa                                                                                                                                       >  1:220542/1‑140 (MQ=255)            gcgcTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAgg                                                                                                                             >  1:244605/1‑140 (MQ=255)             cgcTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAggg                                                                                                                            >  1:581634/1‑140 (MQ=255)             cgcTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAggg                                                                                                                            >  1:245240/1‑140 (MQ=255)             cgcTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAggg                                                                                                                            <  2:581634/140‑1 (MQ=255)             cgcTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAggg                                                                                                                            >  2:469743/1‑140 (MQ=255)             cgcTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAggg                                                                                                                            >  1:606557/1‑140 (MQ=255)               cTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGaa                                                                                                                          >  1:334144/1‑140 (MQ=255)                tGGTGAATAATCGGGTTTGGCGCAGGGCGGCGCTGAAAGTGCAGGAGGCCCGGGCGCAATACCGTCTGCCCGATGCCGCCCGCTACCCACAGCTCAATGGGGAGGGCAGAGGCAGCTGGAGCTGTAATCTAAAAGGGAAt                                                                                                                         <  2:1295623/140‑1 (MQ=255)                tGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAAt                                                                                                                         <  2:606557/140‑1 (MQ=255)                 ggTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATa                                                                                                                        <  2:998299/140‑1 (MQ=255)                       taatCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCa                                                                                                                  <  2:637057/140‑1 (MQ=255)                          tCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGa                                                                                                               <  1:1741409/140‑1 (MQ=255)                            gggATTTGCGCATGGCGCCGCTAAAAGTGCGGGAGGCCCGGGCGAAATATCGTCTAACCGATGCCGCCGGCTACCCGCCGCTCAATGGCAAGGGCAGCGGCAGGTGGAGCTGTAATCTTAAGGGGAATACCGCCACGaca                                                                                                             <  2:71877/140‑1 (MQ=255)                            gggATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGaca                                                                                                             >  1:427679/1‑140 (MQ=255)                            gggATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGaca                                                                                                             >  1:1280249/1‑140 (MQ=255)                            gggATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGaca                                                                                                             >  1:1665198/1‑140 (MQ=255)                            gggATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCAAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGaca                                                                                                             >  1:71877/1‑140 (MQ=255)                               aTTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACgg                                                                                                          >  2:1337110/1‑140 (MQ=255)                                  tGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAg                                                                                                       >  2:792297/1‑140 (MQ=255)                                        tGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGCGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCg                                                                                                 >  2:1150494/1‑140 (MQ=255)                                         ggCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGATCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGa                                                                                                <  1:745453/140‑1 (MQ=255)                                          gCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGAGAGTTTTCGAc                                                                                               <  2:1077140/140‑1 (MQ=255)                                           cGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGAcc                                                                                              >  1:1290422/1‑140 (MQ=255)                                           cGACGCTGAAAGTGCAGGAGGCACGGGCGAAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGAcc                                                                                              <  2:427679/140‑1 (MQ=255)                                            gACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCg                                                                                             <  1:1279170/140‑1 (MQ=255)                                            gACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCg                                                                                             <  2:245240/140‑1 (MQ=255)                                            gACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCg                                                                                             <  2:334144/140‑1 (MQ=255)                                                   aaaGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGATACGGGAGTTTTCGACCGGCCTTaa                                                                                      >  1:364341/1‑140 (MQ=255)                                                             aggCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCttt                                                                            >  1:913461/1‑140 (MQ=255)                                                                      cgcAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGAt                                                                   >  1:1543567/1‑140 (MQ=255)                                                                             tCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCg                                                            <  1:792297/140‑1 (MQ=255)                                                                              cGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCgg                                                           <  2:1727254/140‑1 (MQ=255)                                                                              cGTCTCACCGATGCCGACCGCTACCCACGGCTCAATGGGGGGGGCGGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCgg                                                           <  2:1280249/140‑1 (MQ=255)                                                                               gTCTGCCCGATGCCGACCGCTCCCCACAGCTCAATGGCGAGGGCAGGGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGt                                                          <  2:1290422/140‑1 (MQ=255)                                                                               gTCTGACCGATGCCGCCCGCTACCCACAGCTCAATGGCGGGGGCAGCGGCAGCTGGAGCTGTAATTTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGt                                                          <  2:36308/140‑1 (MQ=255)                                                                               gTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGt                                                          <  2:1543567/140‑1 (MQ=255)                                                                                tCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTc                                                         >  2:960584/1‑140 (MQ=255)                                                                                 cTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGATACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCg                                                        <  2:364341/140‑1 (MQ=255)                                                                                   gACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCt                                                      <  2:1284032/140‑1 (MQ=255)                                                                                   gACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCt                                                      >  1:1441392/1‑140 (MQ=255)                                                                                    aCCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACGCGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTc                                                         <  1:643860/136‑1 (MQ=255)                                                                                    aCCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACGCGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTc                                                         >  2:643860/1‑136 (MQ=255)                                                                                       gtggCCGACCGCTCCCCACGGCT‑AAAGGGGGGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAg                                                 <  2:1605813/137‑1 (MQ=255)                                                                                        aTGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAg                                                 <  1:1030822/140‑1 (MQ=255)                                                                                         tGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGa                                                >  2:939550/1‑140 (MQ=255)                                                                                         tGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGa                                                >  2:603160/1‑140 (MQ=255)                                                                                              aCCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGt                                                          >  1:942408/1‑125 (MQ=255)                                                                                              aCCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGt                                                          <  2:942408/125‑1 (MQ=255)                                                                                                   tACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGa                                      >  2:801730/1‑140 (MQ=255)                                                                                                            cTCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCg                             >  2:1111887/1‑140 (MQ=255)                                                                                                             tCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGa                            <  2:1448299/140‑1 (MQ=255)                                                                                                             tCAATGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGa                            <  1:603160/140‑1 (MQ=255)                                                                                                               aaTGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGACa                          >  1:1217545/1‑140 (MQ=255)                                                                                                               aaTGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGACa                          >  2:949435/1‑140 (MQ=255)                                                                                                               aaTGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGACa                          >  1:799124/1‑140 (MQ=255)                                                                                                               aaTGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGACa                          >  1:45620/1‑140 (MQ=255)                                                                                                                aTGGCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGACaa                         >  2:125155/1‑140 (MQ=255)                                                                                                                   gCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGACAAAat                      <  2:913461/140‑1 (MQ=255)                                                                                                                   gCGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAACGACAAAat                      <  2:222222/140‑1 (MQ=255)                                                                                                                    cGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGACAAAatt                     >  2:1417161/1‑140 (MQ=255)                                                                                                                    cGAGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGACAAAatt                     >  1:1139551/1‑140 (MQ=255)                                                                                                                      aGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGa                            >  2:601889/1‑131 (MQ=255)                                                                                                                      aGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGa                            <  1:601889/131‑1 (MQ=255)                                                                                                                      aGGGCAGCGGCAGCTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGACAAAattat                   >  2:1164422/1‑140 (MQ=255)                                                                                                                            gcggcagcTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGACAAAATTATTTAGcc             <  1:949435/140‑1 (MQ=255)                                                                                                                            gcggcagcTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGACAAAATTATTTAGcc             <  1:801730/140‑1 (MQ=255)                                                                                                                             gggaggcTGGAGCTGTATTCTTAACGGAAATGCCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGACAAAATTATTTAGCCa            <  2:45620/135‑1 (MQ=255)                                                                                                                             cggcagcTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGACAAAATTATTTAGCCa            >  2:1188563/1‑140 (MQ=255)                                                                                                                             cggcagcTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGACAAAATTATTTAGCCa            >  1:266459/1‑140 (MQ=255)                                                                                                                             cggcagcTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGACAAAATTATTTAGCCa            <  1:1188563/140‑1 (MQ=255)                                                                                                                             cggcagcTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGCTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGACAAAATTATTTAGCCa            >  1:837160/1‑140 (MQ=255)                                                                                                                              ggcagcTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGACAAAATTATTTAGCCAc           <  1:960584/140‑1 (MQ=255)                                                                                                                              ggcagcTGGAGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGACAAAATTATTTAGCCAc           <  1:1111887/140‑1 (MQ=255)                                                                                                                                       aGCTGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGACAAAATTATTTAGCCACTGAGGAAGc  <  2:1217545/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         TGATTAGCGAGGCGCTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGAAGCCGAGCGACAAAATTATTTAGCCACTGAGGAAGC  >  NC_000913/595796‑596069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑