Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	936,953	G→T	D1244Y (GAT→TAT)	ftsK →	cell division DNA translocase FtsK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	936,953	0	G	T	100.0%	286.2 / NA	80	D1244Y (GAT→TAT)	ftsK	cell division DNA translocase FtsK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (46/34);  total (46/34)

GCTGGGTATGGGGGATATGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCAAAGCGTCAATTTCTGGCGTACAGC  >  NC_000913/936817‑937088                                                                                                                                         |                                                                                                                                        gctgGGTATGGGGGATATGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATg                                                                                                                                      <  2:145943/140‑1 (MQ=255)    gggTATGGGGGATATGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCa                                                                                                                                   >  2:167295/1‑140 (MQ=255)    gggTATGGGGGATATGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCa                                                                                                                                   <  2:874986/140‑1 (MQ=255)    gggTATGGGGGATATGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCa                                                                                                                                   <  1:164100/140‑1 (MQ=255)      gTATGGGGGATATGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATc                                                                                                                                 <  1:1377060/140‑1 (MQ=255)          gggggATATGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCAt                                                                                                                                  <  2:1338219/135‑1 (MQ=255)          gggggATATGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCAt                                                                                                                                  >  1:1338219/1‑135 (MQ=255)            gggATATGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTcc                                                                                                                           >  1:603179/1‑140 (MQ=255)               atatGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGAc                                                                                                                        >  1:1643416/1‑140 (MQ=255)               atatGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGAc                                                                                                                        >  2:548902/1‑140 (MQ=255)                 atGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACag                                                                                                                      >  1:1430189/1‑140 (MQ=255)                 atGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACag                                                                                                                      >  1:378567/1‑140 (MQ=255)                 atGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACag                                                                                                                      >  1:261917/1‑140 (MQ=255)                  tGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTTCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACagc                                                                                                                     <  2:1430189/140‑1 (MQ=255)                   gCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACagcg                                                                                                                    <  2:886824/140‑1 (MQ=255)                       tACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACagcgaaag                                                                                                                <  2:866292/140‑1 (MQ=255)                         ctctGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACagcgaaagcg                                                                                                              >  2:1016240/1‑140 (MQ=255)                            tGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAg                                                                                                           >  1:1348993/1‑140 (MQ=255)                            tGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAg                                                                                                           <  1:1016240/140‑1 (MQ=255)                              ggCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAggt                                                                                                         >  2:1262466/1‑140 (MQ=255)                              ggCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAggt                                                                                                         >  2:360129/1‑140 (MQ=255)                              ggCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAggg                                                                                                         >  2:105589/1‑139 (MQ=255)                              ggCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAggg                                                                                                         >  2:848948/1‑139 (MQ=255)                               gCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAggtg                                                                                                        >  2:516899/1‑140 (MQ=255)                                  gAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGgtggtg                                                                                                     <  2:1348993/140‑1 (MQ=255)                                   aaCTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGc                                                                                                    >  1:1235399/1‑140 (MQ=255)                                   aaCTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGc                                                                                                    >  2:838918/1‑140 (MQ=255)                                      tCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCggg                                                                                                 <  2:1235399/140‑1 (MQ=255)                                        cACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCggg                                                                                                 <  2:1019419/138‑1 (MQ=255)                                        cACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCggg                                                                                                 >  1:1019419/1‑138 (MQ=255)                                               ccGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGa                                                                                        <  1:548902/140‑1 (MQ=255)                                                       tgtCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGGGGGTGGTTTCGATGGCGCTg                                                                                >  2:1476889/1‑140 (MQ=255)                                                         tCCATGGTGCTTTTGTTCGCGATCAGGAAGTACATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGgcg                                                                                   >  1:1356783/1‑135 (MQ=255)                                                         tCCATGGTGCTTTTGTTCGCGATCAGGAAGTACATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGgcg                                                                                   <  2:1356783/135‑1 (MQ=255)                                                            aTGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATgg                                                                                     <  1:603487/130‑1 (MQ=255)                                                            aTGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATgg                                                                                     >  2:603487/1‑130 (MQ=255)                                                            aTGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTgaagaa                                                                           <  1:426117/140‑1 (MQ=255)                                                                 gCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGCGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGa                                                                      >  2:1401933/1‑140 (MQ=255)                                                                  cTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGAt                                                                     >  1:715458/1‑140 (MQ=255)                                                                    tttGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATcc                                                                   <  1:714011/140‑1 (MQ=255)                                                                    tttGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATcc                                                                   <  1:360129/140‑1 (MQ=255)                                                                     ttGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCg                                                                  <  2:1611521/140‑1 (MQ=255)                                                                       gTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCgtt                                                                >  1:803684/1‑140 (MQ=255)                                                                        ttCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCgttg                                                               >  2:1737515/1‑140 (MQ=255)                                                                        ttCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCgttg                                                               >  2:1133839/1‑140 (MQ=255)                                                                         tCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCgttgt                                                              >  2:1345393/1‑140 (MQ=255)                                                                          cgcgATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCgttgtt                                                             >  2:723755/1‑140 (MQ=255)                                                                          cgcgATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCgttgtt                                                             >  2:105449/1‑140 (MQ=255)                                                                          cgcgATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCgttgtt                                                             <  1:105449/140‑1 (MQ=255)                                                                          cgcgATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCgttgtt                                                             <  1:516899/140‑1 (MQ=255)                                                                                 aGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAg                                                      <  1:354632/140‑1 (MQ=255)                                                                                 aGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAg                                                      <  1:551971/140‑1 (MQ=255)                                                                                  ggAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAgg                                                     <  2:1128553/140‑1 (MQ=255)                                                                                   gAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGc                                                    <  2:1283559/140‑1 (MQ=255)                                                                                   gAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGc                                                    <  1:1722732/140‑1 (MQ=255)                                                                                         cATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCa                                              >  2:1118618/1‑140 (MQ=255)                                                                                           tGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGt                                            >  2:898429/1‑140 (MQ=255)                                                                                           tGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGt                                            >  2:1056569/1‑140 (MQ=255)                                                                                                 ggtgCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCa                                      >  1:72979/1‑140 (MQ=255)                                                                                                 ggtgCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCa                                      >  2:1702691/1‑140 (MQ=255)                                                                                                          cTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAAc                             >  2:1219263/1‑140 (MQ=255)                                                                                                          cTGGAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAAc                             >  1:1678163/1‑140 (MQ=255)                                                                                                             gAAAGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCa                          >  2:1555215/1‑140 (MQ=255)                                                                                                               aaGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCaaa                        <  1:724285/140‑1 (MQ=255)                                                                                                               aaGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCaaa                        <  2:662953/140‑1 (MQ=255)                                                                                                                aGCGCGTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCAAAg                       <  2:1485063/140‑1 (MQ=255)                                                                                                                  cgcgTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGGGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCAAAGCg                     <  1:1476889/140‑1 (MQ=255)                                                                                                                  cgcgTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCAAAGCg                     >  1:1019747/1‑140 (MQ=255)                                                                                                                  cgcgTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCAAAGCg                     >  1:1561199/1‑140 (MQ=255)                                                                                                                  cgcgTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCAAAGCg                     >  2:723908/1‑140 (MQ=255)                                                                                                                    cgTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCAAAGCGTc                   <  1:1737515/140‑1 (MQ=255)                                                                                                                    cgTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCAAAGCGTc                   <  1:1133839/140‑1 (MQ=255)                                                                                                                     gTGGTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCAAAGCGTCa                  >  1:751386/1‑140 (MQ=255)                                                                                                                        gTCGCCCACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCAAAGCGTCAAtt               >  1:492386/1‑140 (MQ=255)                                                                                                                            cccACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCAAAGCGTCAATTTCTg           <  1:1219263/140‑1 (MQ=255)                                                                                                                            cccACAGTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCAAAGCGTCAATTTCTg           >  2:1381243/1‑140 (MQ=255)                                                                                                                                  gTATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCAAAGCGTCAATTTCTGGCGTAc     <  1:838918/140‑1 (MQ=255)                                                                                                                                   tATGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGAATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCAAAGCGTCAATTTCTGGCGTACa    >  1:1365208/1‑140 (MQ=255)                                                                                                                                     tGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCAAAGCGTCAATTTCTGGCGTACAGc  >  2:860693/1‑140 (MQ=255)                                                                                                                                     tGTTTATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCAAAGCGTCAATTTCTGGCGTACAGc  >  2:623620/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        GCTGGGTATGGGGGATATGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCAAAGCGTCAATTTCTGGCGTACAGC  >  NC_000913/936817‑937088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑