Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	646,747	0	T	G	32.6%	63.0 / 28.0	43	T255P (ACC→CCC)	citG	triphosphoribosyl‑dephospho‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base T (2/27);  new base G (14/0);  total (16/27)
Fisher's exact test for biased strand distribution p-value = 1.53e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGC  >  NC_000913/646608‑646879                                                                                                                                            |                                                                                                                                      aaaTTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGt                                                                                                                                       <  2:158352/140‑1 (MQ=255)              gCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCt                                                                                                                         >  1:1345279/1‑140 (MQ=255)               cTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GGATGcccccct                                                                                                                        >  1:1384168/1‑138 (MQ=255)                  aaaTAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GGATTCCCCCctttt                                                                                                                     >  1:605338/1‑140 (MQ=255)                       atTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GGATGCCCCCcttttttcat                                                                                                                >  2:1202792/1‑135 (MQ=255)                         tAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCCTTTTGCaataa                                                                                                              >  1:1270329/1‑140 (MQ=255)                              cTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGttt                                                                                                         <  1:418396/140‑1 (MQ=255)                                gtgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GGATGCCCCCCTTTTTCTATAAAATTTgt                                                                                                       >  2:382005/1‑139 (MQ=255)                                 tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTT‑GGATGCCCCCCTTTTTCCATAAAGTTTtcg                                                                                                      >  2:439253/1‑140 (MQ=255)                                 tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GAATGGCCCCCTTTTTTAATAAAGTTTtcg                                                                                                      >  1:1035763/1‑140 (MQ=255)                                     aaaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTc                                                                                                  <  1:559340/140‑1 (MQ=255)                                        aaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTcgcg                                                                                               <  1:921151/140‑1 (MQ=255)                                                     tCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GGATGCCCCCCTTTTTTTATAATTTTTTCGCCCCCCGCTGTAGCCCgcgc                                                                                  >  2:905544/1‑140 (MQ=255)                                                       aGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTT‑GAATGCCCCCCTTTTTTAATAATGTTTTCGCCCCGCGCCGTGGCCAgcgccg                                                                                >  2:540686/1‑138 (MQ=255)                                                       aGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GGATGCCCCCCTTTTTCAATAATATTTTCGCCCCGCGCCGTAGCCAgcgccg                                                                                >  1:1057523/1‑138 (MQ=255)                                                       aGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GGAAGGCCCCCTTTTGTAATAAAATTTTCGCCCCCCGCTGTAGCCGgcgccg                                                                                >  2:1081515/1‑138 (MQ=255)                                                          aGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGcc                                                                             <  1:439253/140‑1 (MQ=255)                                                          aGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGcc                                                                             <  1:546666/140‑1 (MQ=255)                                                          aGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGcc                                                                             <  2:1345279/140‑1 (MQ=255)                                                             tCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGGC‑GGAGGCCCCCCTTTTTTAATAAATTTTTTGCCCCCCCCTCCAGCCCGCGCCCGccccc                                                                          >  2:1110483/1‑140 (MQ=255)                                                              cAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GAATGCCCCCCTTTTTCAATAATGTTTTCGCCTCCCGCTGTAGCCAGCGCAGGcccccc                                                                         >  1:1144304/1‑140 (MQ=255)                                                                gCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTT‑GGATGCCCCCCTTTTTCAATAATATTTTTGCCTCCCGCTGTGGCCCGCGCAGGcccccccc                                                                       >  2:1150670/1‑138 (MQ=255)                                                                gCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GGAAGCCCCCCTTTTTCAATAATGTTTTCGCCCCCCGCTCTAGCCCACGCAGGcccccccc                                                                       >  2:612041/1‑138 (MQ=255)                                                                 cACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTT‑GGATTCCCCCCTTTTTCTATAATGTTTTTGCCCCCCCGTGTAGCCCGGGCAGGccccccccc                                                                      >  2:1393415/1‑137 (MQ=255)                                                                  aCTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGc                                                                     <  1:905544/140‑1 (MQ=255)                                                                   cTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GAATGCCCCCCTTTTTTAAAAATATTTTTGCCTCCCCCCGTAGCCCGCGCCGGccccccccccc                                                                    >  2:1234255/1‑135 (MQ=255)                                                                         ccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgcg                                                              <  1:100176/140‑1 (MQ=255)                                                                         ccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgcg                                                              <  2:1270329/140‑1 (MQ=255)                                                                             gACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGAt                                                          <  1:1393415/140‑1 (MQ=255)                                                                              aCTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATg                                                         <  1:382697/140‑1 (MQ=255)                                                                              aCTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATg                                                         <  1:266401/140‑1 (MQ=255)                                                                               cTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGc                                                        <  1:1284980/140‑1 (MQ=255)                                                                               cTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGc                                                        <  1:819695/140‑1 (MQ=255)                                                                                tGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCa                                                       <  2:448058/140‑1 (MQ=255)                                                                                       tcgttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GAATGCCCCCCTTTTTCAATAAATTTTTCGCCCCCCCCCGTAGCCCCCGCAGGCCCCCCCCCCCCCCCCCCGGTGCAACGCTgg                                                >  2:428758/1‑140 (MQ=255)                                                                                          ttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTT‑GAATGCCCCCCTTTTTCAATAATATTTTCGCCTCCCGCCGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCCCGATGCAACGGTGGGAt                                             >  2:1230975/1‑140 (MQ=255)                                                                                            cgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCg                                           <  1:413041/140‑1 (MQ=255)                                                                                                   cTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGa                                    <  1:1150670/140‑1 (MQ=255)                                                                                                   cTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGa                                    <  1:1268145/140‑1 (MQ=255)                                                                                                     cccTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCTAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATc                                  <  1:333292/140‑1 (MQ=255)                                                                                                      ccTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCg                                 <  2:469999/140‑1 (MQ=255)                                                                                                       cTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GGATGCCCCCCTTTTTTAATAAAGTTTTCGCCCCCCCCCGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCCCGCTGCCACGGTGGGATTGCCGGTGGTCGc                                >  2:719354/1‑140 (MQ=255)                                                                                                        tGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGcc                               <  1:981097/140‑1 (MQ=255)                                                                                                         gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCa                              <  2:1057523/140‑1 (MQ=255)                                                                                                             aaCTGCCGGAGATAATCGAGATCGGCGGGGGGTT‑GAATGGCCCCCTTTTTTAATAAATTTTTCGCCCCCCGCCGTTGCCAGCGCCGGGCCCCCCCCCCCCCCCCCGCTGCAACGTTGGTATTGCCGGTTTTCGCCCTCac                          >  2:298123/1‑139 (MQ=255)                                                                                                              aCTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGt                         <  1:719354/140‑1 (MQ=255)                                                                                                                tGCCGGAGATAATCGAGATCGGCGGGGGGTCGGAAGGCCCCCTTTTT‑CCATAAATTTTTCGTCTCGCCCTGGTGACAAGGCACGTCCCCCTCGCCACCGCGCCCTGCCACATTGGGAACGCCCTTTATCCCCCTCaccag                       >  2:562746/1‑136 (MQ=255)                                                                                                                    ggAGATAATCGAGATCGGCGGGGGGTC‑GAATGCCCCCCTTTTTCAATAATTTTTTCGCCCCCCGCTGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCCCGGTGCAAAGTTTGTATCGCCCTTGGTCGCCCTCAGgaggagg                   >  2:1191522/1‑139 (MQ=255)                                                                                                                     gagaTAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCa                  <  1:540686/140‑1 (MQ=255)                                                                                                                       gaTAATCGAGATCGGCGGGGGGTC‑GGATGCCCCCCTTTTTTAATAAAGTTTTTGCCCCGCCCCGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCCCGGTGCAACCGTGGGATCCCCCCTGATCGCCCTCCGGAGGAGGAAg                >  2:825159/1‑140 (MQ=255)                                                                                                                           aTCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTAt            <  2:1384168/140‑1 (MQ=255)                                                                                                                                gaTCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGc       <  2:493567/140‑1 (MQ=255)                                                                                                                                     gcggGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGc  <  2:1144304/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                      AAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGC  >  NC_000913/646608‑646879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑